Patents Assigned to Gesellschaft fur Biotechnologische Forschung mbH
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Patent number: 5338681Abstract: The invention relates to a method for isolating a polysaccharide extracellularly producing bacteria from a mixed culture of non-polysaccharide and polysaccharide producing bacteria, The method uses a streaking technique on a nutrient medium containing unsubstituted gellan as a gellant. The polysaccharide extracellularly producing bacteria are isolated by the act of non-polysaccharide extracellularly producing bacteria sinking into the nutrient medium. However, the polysaccharide extracellularly producing bacteria do not sink into the nutrient medium. The isolated cells are capable of producing an exopolysaccharide such as P4. Further the isolated cells are capable of producing gellan. The specific polysaccharide producing bacteria are of the genera Auromonas, Pseudomonas and Sphingomonas.Type: GrantFiled: March 26, 1992Date of Patent: August 16, 1994Assignee: Gesellschaft fur Biotechnologische Forschung mbH GBFInventors: Wolf-Dieter Deckwer, Detlef Lobas, Adrian Schumpe
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Patent number: 5223224Abstract: A sensor arrangement for flow injection analysis, includes an enzyme reactor and a chemical/physical sensor. The sensor arrangement is designed as a sealed module and a flow channel of great length is accommodated in the module. By this sensor arrangement, gases generated or consumed in the enzyme reactor are detected without interfering gas exchange with the environment.Type: GrantFiled: December 11, 1990Date of Patent: June 29, 1993Assignee: Gesellschaft fur Biotechnologische Forschung mbH (GBF)Inventors: Bernd A. Dremel, Detlef H. Hanisch
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Patent number: 5173165Abstract: The invention relates to an enzyme electrode having a bienzyme system containing fungal peroxidase or horseradish peroxidase and one or more oxidases, and to the use of the electrode.Type: GrantFiled: October 11, 1990Date of Patent: December 22, 1992Assignee: Gesellschaft fur Biotechnologische Forschung mbH (GBF)Inventors: Rolf Schmid, Juozas Kulys
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Patent number: 5154931Abstract: The invention relates to a therapeutic composition (especially for bone lesions with cavity formation and parodontitis) containing a growth-stimulating material and a solid carrier, to such a material and to a process for the preparation thereof.Type: GrantFiled: August 12, 1988Date of Patent: October 13, 1992Assignee: Gesellschaft fur Biotechnologische Forschung mbH (GBF)Inventors: Wolfgang Kruger, Hubert Mayer, Karl G. Kukoschke, Klaus D. Schluter, Gunter Delling
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Patent number: 5096922Abstract: The invention relates to cyclic antibiotics of formula ##STR1## wherein the prefix R represents that the substituents on the adjacent carbon atom are in the R configuration; the prefix S represents that the substituents on the adjacent carbon atom are in the S configuration;R.sub.1 is hydrogen or hydroxy and R.sub.2 is hydroxy and salts thereof, especially pharmaceutically acceptable salts.The compounds of formula I are prepared by fermentation using the strain So ce 12 (NCIB 12134) of the species Sorangium cellulosum and are effective antibiotics.Type: GrantFiled: April 23, 1990Date of Patent: March 17, 1992Assignee: Gesellschaft fur Biotechnologische Forschung mbHInventors: Hans Reichenbach, Gerhard Hofle, Hermann Augustiniak, Norbert Bedorf, Klaus Gerth, Herbert Irschik, Rolf Jansen, Brigitte Kunze, Dietmar Schomburg, Heinrich Steinmetz, Wolfram Trowitzsch-Kienast, Victor Wray
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Patent number: 5093358Abstract: Compounds of the formula I ##STR1## in which either R is methyl and there is a double bond in the 9,10-position, or in which R is hydrogen and there is a single bond in the 9,10-position, are highly active against Acarina which damage plants.Type: GrantFiled: August 7, 1990Date of Patent: March 3, 1992Assignees: Ciba-Geigy Corp., Gesellschaft fur Biotechnologische Forschung mbHInventors: Marius Sutter, Johannes P. Pachlatko, Gerhard Hofle, Hans Reichenbach
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Patent number: 5087425Abstract: The invention relates to a device for flow-injection analysis having a reaction loop, one or more pumps, an injection valve, one or more detectors and an evaluating device.Type: GrantFiled: August 28, 1989Date of Patent: February 11, 1992Assignee: Gesellschaft fur Biotechnologische Forschung mbH (GBF)Inventors: Josef Flossdorf, Neophytos Papamichael, Detlef Hanisch, Henning Schillig
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Patent number: 5079167Abstract: The invention relates to a method for resolving carbamate racemates of amino acids or norephedrine by enzmatic resolution.Type: GrantFiled: October 8, 1987Date of Patent: January 7, 1992Assignees: Gesellschaft fur Biotechnologische Forschung mbH (GBF), Degussa AG, Zweigniederlassung WolfgangInventors: Clemens Sambale, Maria-Regina Kula, Werner Hummel, Karlheinz Drauz
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Patent number: 5032524Abstract: The invention relates to an apparatus and a process for the bubble-free gassing of liquids, especially of culture media for propagating tissue cultures by means of a porous membrane filament system, whereby liquid media and gas are introduced into the culture. The porous membrane filaments include both hydrophobic and hydrophobic filaments.Type: GrantFiled: May 18, 1987Date of Patent: July 16, 1991Assignee: Gesellschaft fur biotechnologische Forschung mbH (GBF)Inventors: Heino Buntemeyer, Jurgen Lehmann
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Patent number: 5023184Abstract: The invention relates to novel polycyclic alkaloids of formula ##STR1## in which R.sup.1 represents hydrogen or hydroxy, R.sup.2 represents hydrogen or acyl and A and B, independently of one another, each represents C.dbd.O or C--OH, and wherein the dashed line represents a C.dbd.C double bond at the position of the middle bond when A or B represents C.dbd.O, or at the position of the two outer bonds when A or B represents C--OH, a process for the manufacture of these compounds by fermentation of a novel microorganism of the species Myxococcus xanthus, the novel microorganism itself, pharmacetical preparations that contain the novel compounds, and the use of the novel compounds as antibiotics and as tumour-inhibiting agents and for the manufacture of pharmaceutical preparations.Type: GrantFiled: August 13, 1987Date of Patent: June 11, 1991Assignees: Ciba-Geigy Corporation, Gesellschaft fur Biotechnologische Forschung mbHInventors: Hans Reichenbach, Klaus Gerth, Herbert Irschik, Brigitte Kunze, Gerhard Hofle, Hermann Augustiniak, Norbert Bedorf, Rolf Jansen, Wolfram Trowitzsch-Kienast, Heinrich Steinmetz
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Patent number: 4997927Abstract: A process for the purfication of synthetic oligonucleotides is described which drastically simplifies the hitherto very time consuming purification process. The deprotection solution is adsorbed without being concentrated on a carrier having reversed phase properties and optionally ion exchanger properties, the oligonuleotides without lipophilic protecting group are removed, their protecting group is cleaved off from the adsorbed oligonucleotides, the adsorbed oligonucleotides are washed and eluted from the carrier and then purified in a manner known per se.Type: GrantFiled: August 27, 1985Date of Patent: March 5, 1991Assignee: Gesellschaft fur Biotechnologische Forschung mbH (GBF)Inventors: Helmut Blocker, Ronald Frank, Gudrun Heisterberg-Moutsis, Gisela Kurth, Andreas Meyerhans
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Patent number: 4997820Abstract: Novel macrocyclic compounds of the formual I ##STR1## where the dotted line in the 9,10-position is a saturated bond or a double bond, alternatively, and R is hydrogen, methyl or certain acyl radicals, and R.sub.o is hydrogen, halogen, azido, thiol, hydroxyl or --OY, where Y is, among other radicals, an ether radical, a silyl group, a sulfonyl group, a sugar radical or a (thiol)acyl radical, and R being methyl if there is a double bond in the 9,10-position, have microbicidal properties for the control of phytopathogenic microorganisms. They can be employed in the customary formulation for the control of plant disease.Type: GrantFiled: September 8, 1989Date of Patent: March 5, 1991Assignees: Gesellschaft fur Biotechnologische Forschung mbH, Ciba-Geigy CorporationInventors: Marius Sutter, Norbert Bedorf, Gerhard Hofle, Dietmar Schummer, Bettina Bohlendorf
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Patent number: 4987149Abstract: Derived macrocyclic compounds of the formula I ##STR1## where R is hydrogen, methyl or certain acyl groups, the dotted line in the 9,10-position is a saturated bond or, in the event that R is methyl, is also a double bond, and X is a keto group, or a substituted or unsubstituted oxime, hydrazone or semicarbazone, represent effective microbicides for controlling plant diseases. They can be employed in the customary formulation as agrochemical agents.Type: GrantFiled: February 23, 1990Date of Patent: January 22, 1991Assignees: Gesellschaft fur Biotechnologische Forschung mbH, Braunschweig, Ciba-Geigy CorporationInventors: Bettina Bohlendorf, Norbert Bedorf, Gerhard Hofle, Dietmar Schummer, Marius Sutter
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Patent number: 4954517Abstract: A macrocyclic compound of the formula I, ##STR1## in which the substituents have the following meaning: X is hydrogen or methyl, Y is hydrogen, --CHO, --COCH.sub.3, --COCH.sub.2 OCH.sub.3, --COCH.sub.2 OCH.sub.3, --COCH.sub.2 NH.sub.2, benzyl, methyl or a silyl group, and A--B--C is the following C.sub.3 -chain members: ##STR2## in which R.sub.1, R.sub.2 and R.sub.3, independently of one another, are hydrogen, methyl, --CHO, --COCH.sub.2 OCH.sub.3, --COCH.sub.2 OCH.sub.3, --COCH.sub.2 NH.sub.2 or a silyl group, and R.sub.4 and R.sub.5 are hydrogen, halogen or --COO(C.sub.1 --C.sub.3) alkyl, is suitable for controlling and preventing plant diseases. A compound of this type can be employed in a customary manner in the form of plant-protecting agents formulated with carrier substances and extenders.Type: GrantFiled: September 8, 1989Date of Patent: September 4, 1990Assignees: Gesellschaft fur Biotechnologische Forschung mbH, Ciba-Geigy CorporationInventors: Marius Sutter, Bettina Bohlendorf, Norbert Bedorf, Gerhard Hofle
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Patent number: 4940804Abstract: Derived macrocyclic compounds of the formula I ##STR1## where R is hydrogen, methyl or certain acyl groups, the dotted line in the 9,10-position is a saturated bond or, in the event that R is methyl, is also a double bond, and X is a keto group, or a substituted or unsubstituted oxime, hydrazone or semicarbazone, represent effective microbicides for controlling plant diseases. They can be employed in the customary formulation as agrochemical agents.Type: GrantFiled: September 8, 1989Date of Patent: July 10, 1990Assignees: Gesellschaft fur Biotechnologische Forschung mbH, Ciba-Geigy CorporationInventors: Bettina Bohlendorf, Norbert Bedorf, Gerhard Hofle, Dietmar Schummer, Marius Sutter
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Patent number: 4879234Abstract: The invention relates to a process for the purification and, if desired, recovery of formate-dehydrogenase (FDH) from Candida boidinii, in which FDH is subjected to a phase distribution in an aqueous 2-phase system that contains a triazine dyestuff that is bonded to an inert water-soluble polymer.Type: GrantFiled: July 30, 1986Date of Patent: November 7, 1989Assignee: Gesellschaft fur Biotechnologische Forschung mbH (GBF)Inventors: Arno Cordes, Maria-Regina Kula, Karl-Heinz Kroner, Wolfgang Stach
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Patent number: 4870018Abstract: In a process and an apparatus for the bubble-free gassing of liquids, especially of culture media for propagating tissue cultures, in which process and apparatus a gas exchange occurs across an immersed membrane sheet, to achieve simple, effective and as uniform as possible a distribution of the gas introduction and gas desorption without mechanical damage to the liquid constituents, a tubular membrane cage formed by hollow membrane filaments is suspended flexibly in the medium to be gassed and moved in a circular oscillating movement without true rotation. The gassing rate is controlled by altering the angular oscillating movement frequency and/or the radius of the circular path, or alternatively by altering the gas concentration and/or the pressure of the gas flowing into the membrane cage.Type: GrantFiled: March 17, 1986Date of Patent: September 26, 1989Assignee: Gesellschaft fur Biotechnologische Forschung mbH (GBF)Inventor: Jurgen Lehmann
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Patent number: 4863848Abstract: The invention concerns a double-stranded recombinant DNA cloning vector comprising one single insertion site for foreign DNA and one or two labelling sites adjacent or approximate to said insertion site. In the instance of two labelling sites, said sites flank the insertion site. The labelling site(s) can be individually labelled in a fashion that results in the labelling of the 3' end of either strand. The use of the invention in a process affords the ability to individually label only one strand at the 3' end in a simplified method for base sequencing analysis.Type: GrantFiled: April 7, 1986Date of Patent: September 5, 1989Assignee: Gesellschaft fur Biotechnologische Forschung mbH (GBF)Inventors: Helmut Blocker, Ronald Frank, Guido Volckaert
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Patent number: 4845222Abstract: Pyocyanine compounds of the formula: ##STR1## wherein R.sup.1 represents one of the formula --C.sub.1-10 -alkylene-CO.sub.2 -C.sub.1-5 -alkyl;--C.sub.1-10 -alkylene-CO.sub.2 H; and ##STR2## R.sup.2 represents hydrogen or C.sub.1-2 -alkyl and R.sup.1 is attached to the 7 or 8 aromatic ring position. The compounds are useful antibiotics.Type: GrantFiled: July 29, 1987Date of Patent: July 4, 1989Assignee: Gesellschaft fur Biotechnologische Forschung mbh (GBF)Inventors: Michael Morr, Christel Kakoschke, Hsin Tsai, Rita Getzlaff
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Patent number: 4826764Abstract: The invention concerns the intercistronic DNA sequence of the atp operon of Escherichia coli for the subunit c of the ATP synthase, a DNA structure and an expression vector which are characterized by the said intercistronic DNA sequence, and a process for the production of proteins using the same. The DNA sequence enhances the rate of synthesis of the proteins.Type: GrantFiled: July 17, 1985Date of Patent: May 2, 1989Assignee: Gesellschaft fur Biotechnologische Forschung mbHInventor: Walter Sebald