Abstract: The present invention relates to assays for detection of nicotine metabolites, in particular cotinine, in fluid samples and uses of these assays in quantification of smoking habits. The assays comprise contacting a body fluid sample with a cyanogen halide and a pyrazolone compound and detecting a change in light absorbance of the pyrazolone compound which is associated with the presence of nicotine metabolites. Also provided are assay kits including a cyanogen halide or cyanogen halide-precursor(s) and a pyrazolone compound.
Type:
Grant
Filed:
May 13, 2011
Date of Patent:
July 22, 2014
Assignee:
GFC Diagnostics Ltd.
Inventors:
Charles John Knill, John Frederick Kennedy