Abstract: A one piece, biphasic media culture container, as disclosed herein, includes a first compartment for a solid nutrient culture medium, a second compartment for a liquid nutrient culture medium and a common headspace enclosure above and extending between said first and second compartments to provide selective fluid communication therebetween. The first and second compartments are discrete from one another except for the common headspace enclosure and are spatially separated by an air space between their respective inner walls.