Patents Assigned to GINKGO BIOWORKS
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Patent number: 11236373Abstract: Disclosed herein are cells, nucleic acids, and proteins that can be used to produce branched (methyl)lipids, such as 10-methylstearic acids, and compositions that include such lipids. Cells disclosed herein comprise methyltransferase and/or reductase genes from bacteria of the class Gammaproteobacteria, which encode enzymes capable of catalyzing the production of branched (methyl)lipids from unbranched, unsaturated lipids. Saturated branched (methyl)lipids produced using embodiments of the present invention have favorable low-temperature fluidity and favorable oxidative stability, which are desirable properties for lubricants and specialty fluids.Type: GrantFiled: September 20, 2018Date of Patent: February 1, 2022Assignee: Ginkgo Bioworks, Inc.Inventors: Arthur J. Shaw, Hannah Blitzblau, Donald V. Crabtree
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Publication number: 20210403921Abstract: Described in this application are enzymes (e.g., cucurbitadienol synthases (CDS), UDP-glycosyltransferases (UGT), C11 hydroxylases, epoxide hydrolases (EPH), squalene epoxidases, and/or cytochrome P450 reductases), host cells expressing the enzymes, and methods of producing mogrol precursors, mogrol, and/or mogrosides using such host cells.Type: ApplicationFiled: November 9, 2019Publication date: December 30, 2021Applicant: Ginkgo Bioworks, Inc.Inventors: Kim Cecelia Anderson, Jeffrey Ian Boucher, Nicholas Flores, Jaide Jensen, Scott Marr, Matthew McMahon, Dayal Saran, Jue Wang, Jie Zhu
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Patent number: 11041162Abstract: Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical nitrogen sources, such as melamine and cyanamide. Fermentation methods using the genetically engineered organisms are also described. The methods of the invention are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, and pharmaceuticals.Type: GrantFiled: April 23, 2019Date of Patent: June 22, 2021Assignee: Ginkgo Bioworks, Inc.Inventors: Colin R. South, Arthur J. Shaw, IV
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Publication number: 20210147880Abstract: Described herein are chimeric terpene synthases, methods for making chimeric terpene synthases, and methods for making terpenes using the same.Type: ApplicationFiled: February 14, 2019Publication date: May 20, 2021Applicant: Ginkgo Bioworks, Inc.Inventors: Christian Ridley, Jue Wang, Scott Marr
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Patent number: 10975398Abstract: Nucleic acids and cells comprising a methyltransferase gene and/or a reductase gene are disclosed. These nucleic acids and cells may be used to produce branched (methyl)lipids, such as 10-methylstearate.Type: GrantFiled: October 25, 2019Date of Patent: April 13, 2021Assignee: Ginkgo Bioworks, Inc.Inventors: Arthur J. Shaw, Hannah Blitzblau, Donald V. Crabtree
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Publication number: 20210071209Abstract: Aspects of the disclosure relate to biosynthesis of cannabinoids and cannabinoid precursors in recombinant cells and in vitro.Type: ApplicationFiled: October 23, 2020Publication date: March 11, 2021Applicant: Ginkgo Bioworks, Inc.Inventors: Kim Cecelia Anderson, Elena Brevnova, Dylan Alexander Carlin, Brian Carvalho, Nicholas Flores, Katrina Forrest, Matthew McMahon, Massimo Merighi, Gabriel Rodriguez, Emily E. Wrenbeck
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Patent number: 10907188Abstract: The present disclosure provides nucleic acids encoding a Large ATP-binding regulator of the LuxR family (LAL) of transcription factors, vectors and host cells including such nucleic acids, and methods for producing compounds (e.g., polyketides or ?-lactam compounds) with such nucleic acids, vectors, and/or host cells.Type: GrantFiled: April 12, 2017Date of Patent: February 2, 2021Assignee: Ginkgo Bioworks, Inc.Inventors: Brian R. Bowman, Joshua A. V. Blodgett, Gregory L. Verdine, Daniel C. Gray, Jay P. Morgenstern, Lucy Foulston, Keith Robison
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Publication number: 20210010037Abstract: The present disclosure identifies pathways, mechanisms, systems and methods to confer chemoautotrophic production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in organisms such that these organisms efficiently convert inorganic carbon to organic carbon-based products of interest using inorganic energy, such as formate, and in particular the use of organisms for the commercial production of various carbon-based products of interest.Type: ApplicationFiled: July 30, 2020Publication date: January 14, 2021Applicant: Ginkgo Bioworks, Inc.Inventors: Curt R. Fischer, Austin J. Che, Reshma P. Shetty, Jason R. Kelly
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Patent number: 10801045Abstract: The present disclosure identifies pathways, mechanisms, systems and methods to confer chemoautotrophic production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in organisms such that these organisms efficiently convert inorganic carbon to organic carbon-based products of interest using inorganic energy, such as formate, and in particular the use of organisms for the commercial production of various carbon-based products of interest.Type: GrantFiled: January 10, 2018Date of Patent: October 13, 2020Assignee: Ginkgo Bioworks, Inc.Inventors: Curt R. Fischer, Austin J. Che, Reshma P. Shetty, Jason R. Kelly
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Publication number: 20200255809Abstract: The present invention relates to methods and compositions that include enzymes and/or binding polypeptides useful for protecting polymers from damage caused by fatty acids from secreted biological fluids such as sebum or sweat.Type: ApplicationFiled: September 12, 2018Publication date: August 13, 2020Applicant: Ginkgo Bioworks, Inc.Inventors: Patrick Magannig Boyle, Jeffrey Ian Boucher, Michael Gregory Napolitano
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Patent number: 10119975Abstract: Systems, methods, libraries, kits, and computer software tools are provided for designing and producing engineered cells. Such engineered cells can be used for cell state quantification, such as genome, transcriptome and/or proteome quantification. In one aspect, an engineered cell having a plurality of artificially designed oligonucleotides introduced into the genome of the cell is provided. The oligonucleotides are each located in proximity of a gene of interest encoding a protein of interest, and are different from one another. The oligonucleotides can each encode a unique peptide tag for each protein of interest, wherein each peptide tag has a unique quantitatively measurable value such as mass-to-charge ratio which can be quantified by a mass spectrometer. The engineered cell is capable of expressing a plurality of proteins of interest each fused to its corresponding unique peptide tag, wherein each peptide tag is capable of being released therefrom.Type: GrantFiled: November 29, 2016Date of Patent: November 6, 2018Assignee: Ginkgo Bioworks, Inc.Inventors: Reshma Shetty, Thomas F. Knight, Jr., Randall D. Rettberg
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Patent number: 9902980Abstract: The present disclosure identifies pathways, mechanisms, systems and methods to confer chemoautotrophic production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in organisms such that these organisms efficiently convert inorganic carbon to organic carbon-based products of interest using inorganic energy, such as formate, and in particular the use of organisms for the commercial production of various carbon-based products of interest.Type: GrantFiled: October 30, 2012Date of Patent: February 27, 2018Assignee: Ginkgo BioWorks, Inc.Inventors: Curt R. Fischer, Austin J. Che, Reshma P. Shetty, Jason R. Kelly
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Patent number: 9752176Abstract: Methods and devices relate to the isolation of nucleic acids of interest from within a population of nucleic acids such as libraries of nucleic acid sequences.Type: GrantFiled: June 15, 2012Date of Patent: September 5, 2017Assignee: Ginkgo Bioworks, Inc.Inventors: Li-yun A. Kung, Daniel Schindler, Joseph Jacobson
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Patent number: 9506167Abstract: Systems, methods, libraries, kits, and computer software tools are provided for designing and producing engineered cells. Such engineered cells can be used for cell state quantification, such as genome, transcriptome and/or proteome quantification. In one aspect, an engineered cell having a plurality of artificially designed oligonucleotides introduced into the genome of the cell is provided. The oligonucleotides are each located in proximity of a gene of interest encoding a protein of interest, and are different from one another. The oligonucleotides can each encode a unique peptide tag for each protein of interest, wherein each peptide tag has a unique quantitatively measurable value such as mass-to-charge ratio which can be quantified by a mass spectrometer. The engineered cell is capable of expressing a plurality of proteins of interest each fused to its corresponding unique peptide tag, wherein each peptide tag is capable of being released therefrom.Type: GrantFiled: July 27, 2012Date of Patent: November 29, 2016Assignee: GINKGO BIOWORKS, INC.Inventors: Reshma Shetty, Thomas F. Knight, Jr., Randall D. Rettberg
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Publication number: 20130029879Abstract: Systems, methods, libraries, kits, and computer software tools are provided for designing and producing engineered cells. Such engineered cells can be used for cell state quantification, such as genome, transcriptome and/or proteome quantification. In one aspect, an engineered cell having a plurality of artificially designed oligonucleotides introduced into the genome of the cell is provided. The oligonucleotides are each located in proximity of a gene of interest encoding a protein of interest, and are different from one another. The oligonucleotides can each encode a unique peptide tag for each protein of interest, wherein each peptide tag has a unique quantitatively measurable value such as mass-to-charge ratio which can be quantified by a mass spectrometer. The engineered cell is capable of expressing a plurality of proteins of interest each fused to its corresponding unique peptide tag, wherein each peptide tag is capable of being released therefrom.Type: ApplicationFiled: July 27, 2012Publication date: January 31, 2013Applicant: Ginkgo BioWorksInventors: Reshma Shetty, Thomas F. Knight, JR., Randall D. Rettberg
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Patent number: 8349587Abstract: The present disclosure identifies pathways, mechanisms, systems and methods to confer chemoautotrophic production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in organisms such that these organisms efficiently convert inorganic carbon to organic carbon-based products of interest using inorganic energy, such as formate, and in particular the use of organisms for the commercial production of various carbon-based products of interest.Type: GrantFiled: October 31, 2011Date of Patent: January 8, 2013Assignee: Ginkgo BioWorks, Inc.Inventors: Curt R. Fischer, Austin J. Che, Reshma P. Shetty, Jason R. Kelly
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Publication number: 20120070870Abstract: The invention features methods and compositions relating to cells that have been engineered to reduce or eliminate proteins having enzymatic activity that interfere with the expression of a metabolic product.Type: ApplicationFiled: June 1, 2010Publication date: March 22, 2012Applicant: Ginkgo BioWorksInventors: Jeffrey C. Way, Joseph H. Davis
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Publication number: 20120064622Abstract: The present disclosure identifies pathways, mechanisms, systems and methods to confer chemoautotrophic production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in organisms such that these organisms efficiently convert inorganic carbon to organic carbon-based products of interest using inorganic energy, such as formate, and in particular the use of organisms for the commercial production of various carbon-based products of interest.Type: ApplicationFiled: October 31, 2011Publication date: March 15, 2012Applicant: GINKGO BIOWORKSInventors: CURT R. FISCHER, AUSTIN J. CHE, RESHMA P. SHETTY, JASON R. KELLY