Abstract: Biometric authentication of a subject utilizing bone and/or blood vessel (vein or artery) structures is provided. A vertice profile is generated from a non-invasive, multi-dimensional image scan of a subject or a portion thereof. The vertice profile is generated by identifying relative locations of divots and peaks on a surface of a bone structure or by identifying branch intersections and traversals of blood vessels. Additional verification techniques can be used for proof of life (verification of human subject). If authenticated, access to physical or digital/virtual assets is allowed.