Abstract: The carbohydrate processing enzyme PNGase F was catalytically inactivated through mutation. Additional mutations yielded a catalytically inactive carbohydrate-binding protein with lectin-like properties including high affinity and specificity N-linked glycans, O-linked glycans, or both.
Type:
Application
Filed:
April 17, 2015
Publication date:
July 6, 2017
Applicants:
UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC., GLYCOSENSORS AND DIAGNOSTICS, LLC
Inventors:
Kausar N. Samli, Robert J. Woods, Loretta Yang
Abstract: The present invention is includes compositions and methods directed to the multiplexed analysis of carbohydrates and carbohydrate containing compounds. The compositions and methods utilize suspension array technology (SAT) and an array of different carbohydrate binding molecules, each carbohydrate binding molecules with a known carbohydrate binding specificity, to obtain a glycoprofile of the carbohydrate structure(s) in a sample. Each carbohydrate binding molecule of a given specificity is linked to the external surface of a population of individually addressable particles.
Type:
Application
Filed:
March 1, 2012
Publication date:
January 2, 2014
Applicants:
GLYCOSENSORS AND DIAGNOSTICS, LLC., UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC.