Patents Assigned to Glycosynth Limited
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Patent number: 10443084Abstract: Conjugates of 2,3-dihydroxynaphthalene and its derivatives with enzyme cleavable groups are chromogenic substrates that form colored compounds when complexed with metal ions, e.g. iron ions, on cleavage by enzymes, and are useful in microbial detection and identification. The cleavage products form purple or red-brown colored complexes, that can easily be observed by the naked eye. Microbes can be grown in the presence of the substrates and the metal salts that provide the metal ion for complexing with the 2,3-dihydroxynaphthalene product. Substituents in the naphthalene ring may affect the solubility of the substrates and also the diffusibility and color of the metal complexes. Some of the substrates yield soluble complexes on cleavage and are of particular value in liquid growth media. Other substrates produce less soluble complexes that are more suitable for use in solid agar media.Type: GrantFiled: February 27, 2018Date of Patent: October 15, 2019Assignee: GLYCOSYNTH LIMITEDInventors: Hayley Jane Turner, Michael Burton
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Publication number: 20180355403Abstract: Conjugates of 2,3-dihydroxynaphthalene and its derivatives with enzyme cleavable groups are chromogenic substrates that form coloured compounds when complexed with metal ions, e.g. iron ions, on cleavage by enzymes, and are useful in microbial detection and identification. The cleavage products form purple or red-brown coloured complexes, that can easily be observed by the naked eye. Microbes can be grown in the presence of the substrates and the metal salts that provide the metal ion for complexing with the 2,3-dihydroxynaphthalene product. Substituents in the naphthalene ring may affect the solubility of the substrates and also the diffusibility and colour of the metal complexes. Some of the substrates yield soluble complexes on cleavage and are of particular value in liquid growth media. Other substrates produce less soluble complexes that are more suitable for use in solid agar media.Type: ApplicationFiled: February 27, 2018Publication date: December 13, 2018Applicant: Glycosynth LimitedInventors: Hayley Jane Turner, Michael Burton
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Patent number: 9938562Abstract: Conjugates of 2,3-dihydroxynaphthalene and its derivatives with enzyme cleavable groups are chromogenic substrates that form colored compounds when complexed with metal ions, e.g. iron ions, on cleavage by enzymes, and are useful in microbial detection and identification. The cleavage products form purple or red-brown colored complexes, that can easily be observed by the naked eye. Microbes can be grown in the presence of the substrates and the metal salts that provide the metal ion for complexing with the 2,3-dihydroxynaphthalene product. Substituents in the naphthalene ring may affect the solubility of the substrates and also the diffusibility and color of the metal complexes. Some of the substrates yield soluble complexes on cleavage and are of particular value in liquid growth media. Other substrates produce less soluble complexes that are more suitable for use in solid agar media. Methods of synthesizing the substrates are described.Type: GrantFiled: November 3, 2014Date of Patent: April 10, 2018Assignee: GLYCOSYNTH LIMITEDInventors: Hayley Jane Turner, Michael Burton
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Patent number: 9879303Abstract: Chromogenic substrates for ?-D-glucuronidase activity comprising monoglucuronides of some 1,2-dihydroxyaromatic derivatives. When cleaved these form soluble colored conjugates with multivalent metal ions such as iron ions. The substrates may be used in conjunction with chromogenic substrates for other enzymes in microbial detection and identification especially involving liquid media. Microbes can be grown in the presence of the substrates and the compounds providing the metal ion. The substrates are particularly useful for detecting ?-D-glucuronidase-positive E. coli. Synthetic methods for making the compounds are described.Type: GrantFiled: November 3, 2014Date of Patent: January 30, 2018Assignee: GLYCOSYNTH LIMITEDInventors: Hayley Jane Turner, Michael Burton
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Publication number: 20160298167Abstract: Conjugates of 2,3-dihydroxynaphthalene and its derivatives with enzyme cleavable groups are chromogenic substrates that form coloured compounds when complexed with metal ions, e.g. iron ions, on cleavage by enzymes, and are useful in microbial detection and identification. The cleavage products form purple or red-brown coloured complexes, that can easily be observed by the naked eye. Microbes can be grown in the presence of the substrates and the metal salts that provide the metal ion for complexing with the 2,3-dihydroxynaphthalene product. Substituents in the naphthalene ring may affect the solubility of the substrates and also the diffusibility and colour of the metal complexes. Some of the substrates yield soluble complexes on cleavage and are of particular value in liquid growth media. Other substrates produce less soluble complexes that are more suitable for use in solid agar media. Methods of synthesising the substrates are described.Type: ApplicationFiled: November 3, 2014Publication date: October 13, 2016Applicant: Glycosynth LimitedInventors: Hayley Jane Turner, Michael Burton
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Publication number: 20160281127Abstract: Chromogenic substrates for ?-D-glucuronidase activity comprising monoglucuronides of some 1,2-dihydroxyaromatic derivatives. When cleaved these form soluble coloured conjugates with multivalent metal ions such as iron ions. The substrates may be used in conjunction with chromogenic substrates for other enzymes in microbial detection and identification especially involving liquid media. Microbes can be grown in the presence of the substrates and the compounds providing the metal ion. The substrates are particularly useful for detecting ?-D-glucuronidase-positive E. coli. Synthetic methods for making the compounds are described.Type: ApplicationFiled: November 3, 2014Publication date: September 29, 2016Applicant: Glycosynth LimitedInventors: Hayley Jane Turner, Michael Burton