Abstract: A method for producing a lactase-containing composition which is purified by selectively removing protease contaminating the lactase using simple and easy means; a lactase-containing composition; and a dairy product containing the lactase-containing composition. A method for producing a lactase-containing composition having a reduced protease content includes: dissolving a composition containing lactase and protease in an aqueous salt solution having an electric conductivity of from 2 to 45 mS/cm; bringing the resultant solution into contact with an ion exchange resin; and collecting a fraction which is not adsorbed onto the ion exchange resin.

Abstract: Provided is a means for accurately analyzing a protease by electrophoresis. Disclosed is an electrophoretic analysis method for analyzing a protease-containing sample, is characterized by exposing a sample containing a protease to be analyzed, to pH conditions under which the protease is rapidly deactivated, and then subjecting the sample to electrophoresis.

Abstract: Provided is a method for determining activity of arylsulfatase in an aqueous system, which comprises a step in which arylsulfatase is subjected to reaction with a substrate, from which fluorophore or chromophore is liberated by suffering an action of the arylsulfatase, in an aqueous reaction system having high ionic strength. Also, provided are a lactase preparation having a lactase activity of 4,000 NLU/g or more according to the FCC IV method and having an arylsulfatase activity of 0.1% or less of the lactase activity as the basis, in which the arylsulfatase activity has been determined by the method for determining activity of arylsulfatase in an aqueous system according to the fluorescence method of the present invention; a method for producing this preparation; and a dairy product which comprises using this preparation.

Abstract: Provided is a means for accurately analyzing a protease by electrophoresis. Disclosed is an electrophoretic analysis method for analyzing a protease-containing sample, is characterized by exposing a sample containing a protease to be analyzed, to pH conditions under which the protease is rapidly deactivated, and then subjecting the sample to electrophoresis.

Abstract: The present invention relates to a prophylactic/therapeutic drug for urolithiasis containing as an active ingredient L-aldonic acid, a salt thereof, or a lactone form thereof.

Abstract: A prophylactic and remedial preparation for a disease attendant on hyperglycemia, a preparation for depressing the rise in blood sugar, and a wholesome food separately include, as an active ingredient, at least one component selected from the group consisting of L-arabinose, L-fucose, 2-deoxy-D-galactose, D-xylose, L-xylose, D-ribose, D-tagatose, D-ribulose, D-lyxose and D-xylulose.

Abstract: A method for the cooking-free saccharification of starch using an amylase produced by Corticium rolfsii AHU 9627 or its variants. According to the method, even a high viscous suspension of 10% (w/v) or more raw-corn starch is almost completely hydrolyzed within 8 hours. The saccharification is proceeded at a higher temperature and a lower pH compared with those in known methods utilizing other amylases which are able to hydrolyze uncooked starch, so that the propagation of the infectious bacteria which would affect the saccharifying efficiency can be avoided.

Abstract: A physiologically active novel substance designated as Mutastein is disclosed, which has the following specific physiological properties:(1) it has a molecular weight of at least 200,000, and when subjected to gel-filtration with Sephadex G-100 and Sephallose 6B, it will be eluted in the void volume;(2) its ultraviolet spectrum: FIG. 1;(3) its infrared spectrum: FIG. 2;(4) it has a proteinous nature and contains about 10% of saccharide;(5) it is soluble in water and a saline solution, but it will be salted out from a saturated solution containing about 30% of ammonium salfate; and it is insoluble in acetone, ethanol, ethylacetate and benzene,(6) it dissolves in a buffer solution having a pH 7 or over, but it precipitates at a pH of from 3 to 3.5,(7) it is stable when heat-treated at pH 9, at 100.degree. C. for 10 minutes,(8) its elementary analysis: C: about 44%, H: about 7%, and N: about 12%,(9) its color reactions: Phenol-surfuric acid color reaction (orange), and Folin's color reaction (blue).