Abstract: This invention relates to a neutral protease useful for animal tissue cell culture and having the chemical and physical properties disclosed in the body of the specification, characterized in that said protease is produced by aerobically culturing a strain of Bacillus polymyxa deposited at the American Type Culture Collection, Rockville, Md. under accession number ATCC 21993 on a culture medium containing a suitable carbon source and nitrogen source.
Abstract: This invention relates to a neutral protease useful for animal tissue cell culture and having the chemical and physical properties disclosed in the body of the specification, characterized in that said protease is produced by aerobically culturing a strain of Bacillus polymyxa deposited at the American Type Culture Collection, Rockville, Maryland under accession number ATCC 21993 on a culture medium containing a suitable carbon source and nitrogen source.