Abstract: The present invention relates to the rapid and electricity-free, point-of-care, multiplexed detection and quantification of at least one or more nucleic acid sequences from nucleic acids corresponding to a plurality of pathogens or biomarkers using a micropatterned lateral flow device. Rapid and molecular-level sensitive differential diagnosis of a disease condition may be enabled without the need for a delayed laboratory test so that timely treatment can be administered.
Type:
Grant
Filed:
July 28, 2020
Date of Patent:
March 11, 2025
Assignees:
GODX, INC, THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH & HUMAN SERVICES
Inventors:
Chang Hee Kim, Wendy A. Henderson, Sarah K. Abey, Nicolaas H. Fourie, Eric G. Ferguson
Abstract: Disclosed herein are method for separating, amplifying, or detecting a nucleic acid from a sample may comprise contacting a sample lysate with a plurality of buoyant, inorganic, nucleic-acid-capture microspheres. The nucleic-acid-capture microspheres may comprise unicellular hollow microspheres having a diameter between 5 and 300 ?m and/or a true particle density between 0.05 and 0.60 grams/cm3. The microspheres may comprise hollow soda-lime-borosilicate microspheres. In some embodiments, the microspheres comprises hollow soda-lime-borosilicate microspheres surrounded by an amorphous silica shell. Also disclosed are kits for performing the methods.
Type:
Grant
Filed:
December 3, 2018
Date of Patent:
September 5, 2023
Assignees:
GoDx, Inc., The United States of America, as Represented by the Secretary, Dep. of Health and Human Services
Inventors:
Chang Hee Kim, Lichen Xiang, Wendy A. Henderson, Xiao Jiang
Abstract: Disclosed herein are method for separating, amplifying, or detecting a nucleic acid from a sample may comprise contacting a sample lysate with a plurality of buoyant, inorganic, nucleic-acid-capture microspheres. The nucleic-acid-capture microspheres may comprise unicellular hollow microspheres having a diameter between 5 and 300 ?m and/or a true particle density between 0.05 and 0.60 grams/cm3. The microspheres may comprise hollow soda-lime-borosilicate microspheres. In some embodiments, the microspheres comprises hollow soda-lime-borosilicate microspheres surrounded by an amorphous silica shell. Also disclosed are kits for performing the methods.
Type:
Application
Filed:
December 3, 2018
Publication date:
June 17, 2021
Applicants:
GoDx, Inc, The United States of America, as Represented by the Secretary, Department of Health and Human Servic
Inventors:
Chang Hee KIM, Lichen XIANG, Wendy A. HENDERSON, Xiao JIANG
Abstract: The present invention relates to the rapid and electricity-free, point-of-care, multiplexed detection and quantification of at least one or more nucleic acid sequences from nucleic acids corresponding to a plurality of pathogens or biomarkers using a micropatterned lateral flow device. Rapid and molecular-level sensitive differential diagnosis of a disease condition may be enabled without the need for a delayed laboratory test so that timely treatment can be administered.
Type:
Application
Filed:
July 28, 2020
Publication date:
November 12, 2020
Applicants:
GODX, INC, THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH & HUMAN SERVICES
Inventors:
Chang Hee Kim, Wendy A. Henderson, Sarah K. Abey, Nicolaas H. Fourie, Eric G. Ferguson
Abstract: The present invention relates to the rapid and electricity-free, point-of-care, multiplexed detection and quantification of at least one or more nucleic acid sequences from nucleic acids corresponding to a plurality of pathogens or biomarkers using a micropatterned lateral flow device. Rapid and molecular-level sensitive differential diagnosis of a disease condition may be enabled without the need for a delayed laboratory test so that timely treatment can be administered.
Type:
Application
Filed:
May 16, 2016
Publication date:
May 31, 2018
Applicants:
GODX, INC, THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH & HUMAN SERVIC
Inventors:
Chang Hee Kim, Wendy A. Henderson, Sarah K. Abey, Nicolaas H. Fourie, Eric G. Ferguson