Abstract: The present invention mainly relates to a process for isolation and purification of yolk antibodies from egg yolk of an anseriform bird by an adsorption chromatographic procedure using a water insoluble non-charged absorbent to accomplish a desired separation of yolk antibodies, and by a salting-out procedure that differentially precipitates the IgY antibodies. The present invention also relates to the yolk antibodies produced thereby and various uses of such yolk antibodies.
Abstract: This invention relates to a method for selectively isolating IgY(&Dgr;Fc) avian antibodies from IgY avian antibodies. For example, the method includes: (a) providing an aqueous fraction from yolk of an egg of an anseriform bird; (b) precipitation IgY antibodies using a first precipitant salt; and (c) precipitating the IgY(&Dgr;Fc) antibodies from a supernatant using a second precipitant salt to provide an isolated preparation enriched for IgY(&Dgr;Fc) antibodies.
Abstract: The present invention relates to a method for the preparation and purification of IgY(&Dgr;Fc) antibody from avian yolk, generally comprising the steps of immunization of a fowl hen with an antigen, a partial purification of the whole antibodies from the eggs laid by the hen, and an immunoaffinity purification of the antibodies raised against the antigen, in which the binding of the antibodies with the antigen in the immunoaffinity purification step is conducted at pH within a range of 4-7 and under an ionic strength of lower than 50 mM. The present invention also relates to the IgY(&Dgr;Fc) antibody produced thereby and various uses of the novel IgY(&Dgr;Fc) antibody.