Abstract: A fluidic device has a culture chamber configured to house a 3D culture matrix comprising a culture of microorganisms. A concentration gradient of a test substance is established over the 3D culture matrix by providing respective fluid flows at different end portions of the culture chamber and comprising different concentrations of the test substance. The response of the microorganisms to the test substance is determined based on the position of a border zone in the 3D culture matrix.

Abstract: A fluidic device (1) has a culture chamber (10) configured to house a 3D culture matrix (2) comprising a culture of microorganisms (6). A concentration gradient of a test substance is established over the 3D culture matrix (2) by providing respective fluid flows at different end portions (12, 14) of the culture chamber (10) and comprising different concentrations of the test substance. The response of the microorganisms (6) to the test substance is determined based on the position of a border zone (5) in the 3D culture matrix (2).

Abstract: A microfluidic capsule (1) comprises a top lid (100), a middle piece (200) and a bottom piece (300) to be assembled to enclose a microfluidic substrate (400) for analysis of cells and biochemical reactions. The middle piece (200) comprises support structures in the form of support pillars (250) and walls (240) around a central light window (220) to provide mechanical support and prevent tension-induced structural deformations. When fully assembled, light windows (120, 220, 230) in the top lid (100), middle piece (200) and bottom piece (300) allows inspection of biological and/or biochemical samples positioned in the enclosed microfluidic substrate (400).

Abstract: A microfluidic capsule (1) comprises a top lid (100), a middle piece (200) and a bottom piece (300) to be assembled to enclose a microfluidic substrate (400) for analysis of cells and biochemical reactions. The middle piece (200) comprises support structures in the form of support pillars (250) and walls (240) around a central light window (220) to provide mechanical support and prevent tension-induced structural deformations. When fully assembled, light windows (120, 220, 230) in the top lid (100), middle piece (200) and bottom piece (300) allows inspection of biological and/or biochemical samples positioned in the enclosed microfluidic substrate (400).