Patents Assigned to GRAPE KING BIO LTD.
  • Patent number: 11766465
    Abstract: The present disclosure relates to as external composition for wound healing containing a Lactobacillus fermentation product, which comprises a Lactobacillus fermentation product as an effective component. The Lactobacillus fermentation product is a bacteria-free concentrated filtrate from fermentation of Lactobacillus plantarum and the effective component is loaded onto a pharmaceutically acceptable absorbent carrier or carrying agent. The external composition for wound healing has anti-inflammatory and healing promoting effects on a skin wound after applied directly onto the skin wound.
    Type: Grant
    Filed: October 29, 2021
    Date of Patent: September 26, 2023
    Assignee: GRAPE KING BIO LTD
    Inventors: Hsing-Chun Kuo, Chin-Chu Chen, Yen-Lien Chen, Shih-Wei Lin, Yen-Po Chen, Ci-Sian Wang, Yu-Hsin Hou, Yang-Tzu Shih, Ching-Wen Lin, Ya-Jyun Chen, Jia-Lin Jiang, You-Shan Tsai, Zi-He Wu
  • Patent number: 11752177
    Abstract: The present invention is related to a method of reducing tobacco addiction in a smoking subject with Lactobacillus fermentum GKF3, including daily administering an oral composition containing the Lactobacillus fermentum GKF3 to the smoking subject, in which the oral composition reduces the total amount of the nicotine metabolites in urine.
    Type: Grant
    Filed: December 17, 2021
    Date of Patent: September 12, 2023
    Assignee: GRAPE KING BIO LTD
    Inventors: Chin-Chu Chen, Yen-Lien Chen, Shih-Wei Lin, Yen-Po Chen, Ci-Sian Wang, Yu-Hsin Hou, Yang-Tzu Shih, Ching-Wen Lin, Ya-Jyun Chen, Jia-Lin Jiang, You-Shan Tsai, Zi-He Wu
  • Patent number: 11730783
    Abstract: The present invention provides an active substance of Morchella, its use and a composition thereof for improving disturbance of reproductive function, especially, for manufacturing a pharmaceutical composition to improve disturbance of reproductive function induced by obesity or metabolic syndrome. The composition with the active substance of Morchella can effectively improve the structural integrity of testicular tissues and sperm, increase testosterone levels in the blood, and reduce the oxidation stress in sperms.
    Type: Grant
    Filed: June 16, 2021
    Date of Patent: August 22, 2023
    Assignee: GRAPE KING BIO LTD.
    Inventors: Chin-Chu Chen, Szu-Yin Wu, Hsiao-Ling Chang, Yen-Po Chen
  • Patent number: 11648233
    Abstract: The present invention is related to an active substance of Hericium erinaceus having a pain-relieving effect, and a pharmaceutical composition including the active substance. The active substance is prepared using the following steps: (a) inoculating a mycelium of H. erinaceus on an agar plate and incubating at 15-32° C. for 8-16 days; (b) inoculating the incubated H. erinaceus mycelia from step (a) into a medium in a flask and incubating at 20-30° C. and pH 4.5-6.5 for 3-5 days; (c) inoculating the incubated H. erinaceus mycelia from step (b) into a medium in a fermentation tank and incubating at 24-32° C. and pH 4.5-5.5 for 8-16 days to obtain a fermented medium of the H. erinaceus mycelia; and (d) desiccating the fermented medium of the H. erinaceus mycelia from step (c) to obtain the powder of the H. erinaceus mycelia, which is further purified and isolated to obtain a novel compound of H. erinaceus.
    Type: Grant
    Filed: November 5, 2019
    Date of Patent: May 16, 2023
    Assignee: GRAPE KING BIO LTD.
    Inventors: Pei-Shan Liu, Chien-Chih Chen, Chin-Chu Chen, Li-Ya Lee, Wan-Ping Chen, Ting-Wei Lin, Jui-Hsia Hsu, Wei-Ching Chu
  • Patent number: 11590180
    Abstract: The present invention provides a Bifidobacterium lactis GKK2, a composition comprising thereof and its use for improving asthma, especially for lowering airway resistance, reducing levels of non-specific and specific IgE in serum, alleviating bronchoconstriction and increasing levels of GSH reductase in liver tissue interstitial fluid to improve allergic asthma.
    Type: Grant
    Filed: August 15, 2019
    Date of Patent: February 28, 2023
    Assignee: GRAPE KING BIO LTD.
    Inventors: Chin-Chu Chen, Yen-Lien Chen, Shin-Wei Lin, Yen-Po Chen, Ci-Sian Wang, Yu-Hsin Hou, Yang-Tzu Shih, Ching-Wen Lin, Ya-Jyun Chen, Jia-Lin Jiang
  • Publication number: 20220143114
    Abstract: The present invention provides an active substance of Lactic Acid Bacteria, a composition comprising thereof and its use for promoting longevity, especially for increasing Cisd2 gene expression, reducing mitochondrial damage and delaying aging conditions such as nerve degeneration and sarcopenia.
    Type: Application
    Filed: January 25, 2022
    Publication date: May 12, 2022
    Applicant: GRAPE KING BIO LTD.
    Inventors: Chin-Chu CHEN, Yen-Lien CHEN, Shin-Wei LIN, Yen-Po CHEN, Ci-Sian WANG, Yu-Hsin HOU, Yang-Tzu SHIH, Ching-Wen LIN, Ya-Jyun CHEN, Jia-Lin JIANG
  • Publication number: 20220118036
    Abstract: The present invention provides a novel use of Morchella active substance. The Morchella active substance is for manufacturing a composition for ameliorating Sarcopenia. The preparation method of the Morchella active substance comprises the following steps: (a) culturing Morchella mycelium in a plate media between 15° C. and 30° C. for 1 to 2 weeks; (b) inoculating the Morchella mycelium cultured in step (a) to a flask and culturing the Morchella mycelium between 15° C. and 30° C. with a pH of 2 to 6 for 4 to 7 days; and (c) inoculating the Morchella mycelium cultured in step (b) to a fermentation tank and culturing the Morchella mycelium between 15° C. and 30° C. with a pH of 2 to 6 for 6 to 10 days, so as to obtain a Morchella mycelium fermentation broth containing the Morchella active substance.
    Type: Application
    Filed: March 11, 2021
    Publication date: April 21, 2022
    Applicant: GRAPE KING BIO LTD.
    Inventors: Chin-Chu CHEN, I-Chen LI, Tsung-Ju LI, Ting-Yu LU, Lynn-Huey CHIANG, Yen-Po CHEN
  • Patent number: 11253556
    Abstract: The present invention provides a Lactobacillus fermentum GKF3, a composition comprising the strain and their use, in which the aforementioned Lactobacillus fermentum GKF3, deposited with accession numbers of BCRC 910824 and CGMCC 15203, can increase the levels of dopamine or/and serotonin in brain tissues, thereby improving the symptoms of the psychataxia such as decreased focus.
    Type: Grant
    Filed: November 6, 2019
    Date of Patent: February 22, 2022
    Assignee: GRAPE KING BIO LTD
    Inventors: Chin-Chu Chen, Yen-Lien Chen, Shih-Wei Lin, Szu-Yin Wu, Yen-Po Chen, Yu-Hsin Hou, Ci-Sian Wang, Yang-Tzu Shih, Jia-Lin Jiang
  • Patent number: 11197901
    Abstract: The present invention provides an active substance of Lactic Acid Bacteria, a composition comprising thereof and its use for promoting longevity, especially for increasing Cisd2 gene expression, reducing mitochondrial damage and delaying aging conditions such as nerve degeneration and sarcopenia.
    Type: Grant
    Filed: August 15, 2019
    Date of Patent: December 14, 2021
    Assignee: GRAPE KING BIO LTD.
    Inventors: Chin-Chu Chen, Yen-Lien Chen, Shin-Wei Lin, Yen-Po Chen, Ci-Sian Wang, Yu-Hsin Hou, Yang-Tzu Shih, Ching-Wen Lin, Ya-Jyun Chen, Jia-Lin Jiang
  • Patent number: 11185563
    Abstract: The present invention discloses uses of treating, preventing or improving bone diseases by Lactobacillus or a composition including the Lactobacillus. The Lactobacillus and compositions can increase the blood calcium concentration, the trabecular bone volume density, the trabecular thickness, the trabecular number and the bone mineral density of a subject, and reduce trabecular spacing of the subject. Further, the present invention discloses a method for treating a subject diagnosed with a bone disease, through identifying the subject having the bone disease and administering to the subject an effective amount of a composition including at least one of Lactobacillus plantarum GKM3 and Lactobacillus paracasei GKS6, wherein the Lactobacillus plantarum GKM3 is deposited in China General Microbiological Culture Collection Center (CGMCC) with a deposition number of CGMCC 14565 on Aug. 25, 2017, and the Lactobacillus paracasei GKS6 is deposited in CGMCC with a deposition number of CGMCC 14566 on Aug. 25, 2017.
    Type: Grant
    Filed: April 23, 2019
    Date of Patent: November 30, 2021
    Assignee: GRAPE KING BIO Ltd.
    Inventors: Chin-Chu Chen, Yen-Lien Chen, Shin-Wei Lin, Yen-Po Chen, Yang-Tzu Shih, Ching-Wen Lin
  • Publication number: 20210308199
    Abstract: The present invention provides an active substance of Morchella, its use and a composition thereof for improving disturbance of reproductive function, especially, for manufacturing a pharmaceutical composition to improve disturbance of reproductive function induced by obesity or metabolic syndrome. The composition with the active substance of Morchella can effectively improve the structural integrity of testicular tissues and sperm, increase testosterone levels in the blood, and reduce the oxidation stress in sperms.
    Type: Application
    Filed: June 16, 2021
    Publication date: October 7, 2021
    Applicant: GRAPE KING BIO LTD.
    Inventors: Chin-Chu CHEN, Szu-Yin WU, Hsiao-Ling CHANG, Yen-Po CHEN
  • Patent number: 11116807
    Abstract: The present invention provides an active substance of Morchella, its use and a composition thereof for improving disturbance of reproductive function, especially, for manufacturing a pharmaceutical composition to improve disturbance of reproductive function induced by obesity or metabolic syndrome. The composition with the active substance of Morchella can effectively improve the structural integrity of testicular tissues and sperm, increase testosterone levels in the blood, and reduce the oxidation stress in sperms.
    Type: Grant
    Filed: June 27, 2018
    Date of Patent: September 14, 2021
    Assignee: GRAPE KING BIO LTD.
    Inventors: Chin-Chu Chen, Szu-Yin Wu, Hsiao-Ling Chang, Yen-Po Chen
  • Patent number: 11058733
    Abstract: The present invention provides a Bifidobacterium lactis having active substances, a composition comprising the same, and a method of promoting longevity using the same by subjecting the composition to a subject, thereby increasing Cisd2 gene expression, reducing damage of mitochondria, delaying aging-related symptoms including nerve degeneration and sarcopenia, and so on.
    Type: Grant
    Filed: August 16, 2019
    Date of Patent: July 13, 2021
    Assignee: GRAPE KING BIO LTD
    Inventors: Chin-Chu Chen, Yen-Lien Chen, Shih-Wei Lin, Yen-Po Chen, Ci-Sian Wang, Yu-Hsin Hou, Yang-Tzu Shih, Ching-Wen Lin, Ya-Jyun Chen, Jia-Lin Jiang
  • Patent number: 10865377
    Abstract: A method of preparing Cordyceps cicadae mycelium active substances for preventing and/or treating xerophthalmia is provided. The method comprises: (a) culturing a Cordyceps cicadae mycelium in a plate media at 15 to 35° C. for 5 to 14 days; (b) inoculating the mycelium of step (a) to a flask containing liquid media and culturing it at 15 to 35° C. with a pH of 2 to 8 for 3 days; (c) inoculating the mycelium of step (b) to a fermenter tank and culturing it at 15 to 35° C. with a pH of 2 to 8 for 3 days, so as to obtain a Cordyceps cicadae mycelium fermentation liquid; (d) freeze-drying and grating the fermentation liquid, so as to obtain a Cordyceps cicadae mycelium powder; (e) extracting the powder with solvent, so as to obtain a Cordyceps cicadae mycelium extract; and (f) drying the extract, so as to obtained the Cordyceps cicadae mycelium active substances.
    Type: Grant
    Filed: February 14, 2018
    Date of Patent: December 15, 2020
    Assignee: GRAPE KING BIO LTD.
    Inventors: Pei-Cheng Lin, Han-Hsin Chang, Chin-Chu Chen, Shu-Hsing Yeh, Li-Ya Lee, Jui-Hsia Hsu
  • Patent number: 10835563
    Abstract: The present invention provides a method for preventing, postponing or treating ocular diseases, including changes in the anterior/posterior chamber volume, vitreous humour, and/or retinal detachment. The method comprises administering to a subject an effective amount of Cordyceps cicadae mycelium active substances. A method for preparing Cordyceps cicadae mycelium active substances comprises following steps: (a) culturing a Cordyceps cicadae mycelium on a plate medium between 15-35° C. for 5-14 days; (b) inoculating the Cordyceps cicadae mycelium of step (a) into a flask and culturing the mycelium between 15-35° C. and pH 2-8 for 3-7 days; and (c) inoculating the Cordyceps cicadae mycelium of step (b) into a fermenter tank and culturing the mycelium by stirring between 15-35° C. and pH 2-8 for 3-5 days, so as to obtain a Cordyceps cicadae mycelium fermentation liquid containing the Cordyceps cicadae mycelium active substances.
    Type: Grant
    Filed: December 20, 2018
    Date of Patent: November 17, 2020
    Assignee: GRAPE KING BIO LTD.
    Inventors: Chin-Chu Chen, Shu-Hsing Yeh, Li-Ya Lee, Jui-Hsia Hsu
  • Publication number: 20200068815
    Abstract: The present invention is related to an active substance of Hericium erinaceus having a pain-relieving effect, and a pharmaceutical composition including the active substance. The active substance is prepared using the following steps: (a) inoculating a mycelium of H. erinaceus on an agar plate and incubating at 15-32° C. for 8-16 days; (b) inoculating the incubated H. erinaceus mycelia from step (a) into a medium in a flask and incubating at 20-30° C. and pH 4.5-6.5 for 3-5 days; (c) inoculating the incubated H. erinaceus mycelia from step (b) into a medium in a fermentation tank and incubating at 24-32° C. and pH 4.5-5.5 for 8-16 days to obtain a fermented medium of the H. erinaceus mycelia; and (d) desiccating the fermented medium of the H. erinaceus mycelia from step (c) to obtain the powder of the H. erinaceus mycelia, which is further purified and isolated to obtain a novel compound of H. erinaceus.
    Type: Application
    Filed: November 5, 2019
    Publication date: March 5, 2020
    Applicant: GRAPE KING BIO LTD.
    Inventors: Pei-Shan Liu, Chien-Chih Chen, Chin-Chu Chen, Li-Ya Lee, Wan-Ping Chen, Ting-Wei Lin, Jui-Hsia Hsu, Wei-Ching Chu
  • Publication number: 20200061134
    Abstract: The present invention provides a method for preventing, postponing or treating ocular diseases, including changes in the anterior/posterior chamber volume, vitreous humour, and/or retinal detachment. The method comprises administering to a subject an effective amount of Cordyceps cicadae mycelium active substances. A method for preparing Cordyceps cicadae mycelium active substances comprises following steps: (a) culturing a Cordyceps cicadae mycelium on a plate medium between 15-35° C. for 5-14 days; (b) inoculating the Cordyceps cicadae mycelium of step (a) into a flask and culturing the mycelium between 15-35° C. and pH 2-8 for 3-7 days; and (c) inoculating the Cordyceps cicadae mycelium of step (b) into a fermenter tank and culturing the mycelium by stirring between 15-35° C. and pH 2-8 for 3-5 days, so as to obtain a Cordyceps cicadae mycelium fermentation liquid containing the Cordyceps cicadae mycelium active substances.
    Type: Application
    Filed: December 20, 2018
    Publication date: February 27, 2020
    Applicant: GRAPE KING BIO LTD.
    Inventors: Chin-Chu CHEN, Shu-Hsing YEH, Li-Ya LEE, Jui-Hsia HSU
  • Publication number: 20200054696
    Abstract: The present invention provides an active substance of Lactic Acid Bacteria, a composition comprising thereof and its use for promoting longevity, especially for increasing Cisd2 gene expression, reducing mitochondrial damage and delaying aging conditions such as nerve degeneration and sarcopenia.
    Type: Application
    Filed: August 15, 2019
    Publication date: February 20, 2020
    Applicant: GRAPE KING BIO LTD.
    Inventors: Chin-Chu CHEN, Yen-Lien CHEN, Shin-Wei LIN, Yen-Po CHEN, Ci-Sian WANG, Yu-Hsin HOU, Yang-Tzu SHIH, Ching-Wen LIN, Ya-Jyun CHEN, Jia-Lin JIANG
  • Publication number: 20200054695
    Abstract: The present invention provides an active substance of Lactic Acid Bacteria, a composition comprising thereof and its use for promoting longevity, especially for increasing Cisd2 gene expression, reducing mitochondrial damage and delaying aging conditions such as nerve degeneration and sarcopenia.
    Type: Application
    Filed: August 15, 2019
    Publication date: February 20, 2020
    Applicant: GRAPE KING BIO LTD.
    Inventors: Chin-Chu CHEN, Yen-Lien CHEN, Shin-Wei LIN, Yen-Po CHEN, Ci-Sian WANG, Yu-Hsin HOU, Yang-Tzu SHIH, Ching-Wen LIN, Ya-Jyun CHEN, Jia-Lin JIANG
  • Publication number: 20200054691
    Abstract: The present invention provides a Bifidobacterium lactis GKK2, a composition comprising thereof and its use for improving asthma, especially for lowering airway resistance, reducing levels of non-specific and specific IgE in serum, alleviating bronchoconstriction and increasing levels of GSH reductase in liver tissue interstitial fluid to improve allergic asthma.
    Type: Application
    Filed: August 15, 2019
    Publication date: February 20, 2020
    Applicant: GRAPE KING BIO LTD.
    Inventors: Chin-Chu CHEN, Yen-Lien CHEN, Shin-Wei LIN, Yen-Po CHEN, Ci-Sian WANG, Yu-Hsin HOU, Yang-Tzu SHIH, Ching-Wen LIN, Ya-Jyun CHEN, Jia-Lin JIANG