Patents Assigned to Great Basin Scientific
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Patent number: 9896723Abstract: Methods for rapidly detecting clinically relevant mutations in the infectious genome of an agent are disclosed. The methods include use of a novel target and temperature dependent RNase H mediated cleavage of blocked DNA primers to initiate isothermal helicase-dependent amplification of a target sequence such as a sequence in the the rpoB gene.Type: GrantFiled: December 10, 2014Date of Patent: February 20, 2018Assignee: Great Basin Scientific, Inc.Inventors: Brian Hicke, Robert D. Jenison, Madeline West
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Patent number: 9657353Abstract: Methods, materials, and kits for distinguishing a population of cells or organisms truly present in a clinical specimen from contaminating cells or organisms is disclosed. The methods and kits use a suppressor to avoid false positive detection of contaminants in nucleic acid amplification reactions.Type: GrantFiled: June 26, 2015Date of Patent: May 23, 2017Assignee: Great Basin Scientific, Inc.Inventors: Wanyuan Ao, Robert D. Jenison, Jamie Purcell
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Publication number: 20170114393Abstract: Methods, materials, and kits for distinguishing a population of cells or organisms truly present in a clinical specimen from contaminating cells or organisms is disclosed. The methods and kits use a suppressor to avoid false positive detection of contaminants in nucleic acid amplification reactions.Type: ApplicationFiled: June 24, 2016Publication date: April 27, 2017Applicant: GREAT BASIN SCIENTIFIC, INC.Inventors: Wanyuan AO, Robert D. JENISON, Jamie PURCELL
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Patent number: 9434999Abstract: Methods, materials, and kits for distinguishing a population of cells or organisms truly present in a clinical specimen from contaminating cells or organisms is disclosed. The methods and kits use a suppressor to avoid false positive detection of contaminants in nucleic acid amplification reactions.Type: GrantFiled: October 15, 2015Date of Patent: September 6, 2016Assignee: Great Basin Scientific, Inc.Inventors: Wanyuan Ao, Robert D. Jenison, Jamie Purcell
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Patent number: 9359637Abstract: Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex.Type: GrantFiled: November 23, 2015Date of Patent: June 7, 2016Assignee: Great Basin Scientific, Inc.Inventors: Robert D. Jenison, Joshua Klonoski, Anthony R. Torres
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Patent number: 9200312Abstract: Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex.Type: GrantFiled: October 3, 2013Date of Patent: December 1, 2015Assignee: Great Basin Scientific CorporationInventors: Robert D. Jenison, Joshua Klonoski, Anthony R. Torres
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Patent number: 8936921Abstract: Methods for rapidly detecting clinically relevant mutations in the infectious genome of an agent are disclosed. The methods include use of a novel target and temperature dependent RNase H mediated cleavage of blocked DNA primers to initiate isothermal helicase-dependent amplification of a target sequence such as a sequence in the rpoB gene.Type: GrantFiled: June 3, 2013Date of Patent: January 20, 2015Assignee: Great Basin Scientific CorporationInventors: Brian Hicke, Robert D. Jenison, Madeline West
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Publication number: 20140134619Abstract: Composition and methods for amplifying and detecting solution-state polynucleotide targets in a single device are described. In one aspect, a method for a coupled isothermal amplification and detection process utilizes a coated solid support, including a solid substrate, a cationic layer, and a plurality of target-specific probes attached to the coated solid support. Polynucleotide targets in the sample are amplified by an isothermal amplification process involving in situ hybridization onto the coated solid support. The entire process can be carried out with a high degree of specificity under low salt conditions in less than one hour. Further aspects of the present invention include methods for coupled hybridization/detection of polynucleotide targets, coated silicon biosensors optimized for use with the coupled detection systems to provide visual detection of polynucleotide targets under visible light conditions, and kits for practicing in the above described methods.Type: ApplicationFiled: December 17, 2013Publication date: May 15, 2014Applicant: Great Basin ScientificInventor: Robert D. Jenison
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Publication number: 20140038200Abstract: Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex.Type: ApplicationFiled: October 3, 2013Publication date: February 6, 2014Applicant: Great Basin ScientificInventors: Robert D. Jenison, Joshua Klonoski, Anthony R. Torres
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Patent number: 8637250Abstract: Composition and methods for amplifying and detecting solution-state polynucleotide targets in a single device are described. In one aspect, a method for a coupled isothermal amplification and detection process utilizes a coated solid support, including a solid substrate, a cationic layer, and a plurality of target-specific probes attached to the coated solid support. Polynucleotide targets in the sample are amplified by an isothermal amplification process involving in situ hybridization onto the coated solid support. The entire process can be carried out with a high degree of specificity under low salt conditions in less than one hour. Further aspects of the present invention include methods for coupled hybridization/detection of polynucleotide targets, coated silicon biosensors optimized for use with the coupled detection systems to provide visual detection of polynucleotide targets under visible light conditions, and kits for practicing in the above described methods.Type: GrantFiled: May 13, 2010Date of Patent: January 28, 2014Assignee: Great Basin ScientificInventor: Robert Delmar Jenison
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Publication number: 20140017730Abstract: Methods for rapidly detecting clinically relevant mutations in the infectious genome of an agent are disclosed. The methods include use of a novel target and temperature dependent RNase H mediated cleavage of blocked DNA primers to initiate isothermal helicase-dependent amplification of a target sequence such as a sequence in the rpoB gene.Type: ApplicationFiled: June 3, 2013Publication date: January 16, 2014Applicant: GREAT BASIN SCIENTIFICInventors: Brian Hicke, Robert D. Jenison, Madeline West
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Patent number: 8574833Abstract: Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex.Type: GrantFiled: November 26, 2008Date of Patent: November 5, 2013Assignee: Great Basin ScientificInventors: Robert D. Jenison, Joshua Klonoski, Anthony R. Torres
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Publication number: 20110039260Abstract: Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex.Type: ApplicationFiled: November 26, 2008Publication date: February 17, 2011Applicant: Great Basin ScientificInventors: Robert D. Jenison, Joshua Klonoski, Anthony R. Torres
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Publication number: 20090221096Abstract: Thin-film biosensor chips for detecting a target analyte in a biological sample are disclosed. The chips include a solid substrate, an antireflective optical layer, an attachment layer using a non-polymeric silane, and an Fc-specific binding molecule coupled to the non-polymeric silane. Kits containing the chips and methods of using and making the chips are also disclosed.Type: ApplicationFiled: December 13, 2006Publication date: September 3, 2009Applicant: Great Basin ScientificInventor: Anthony R. Torres