Abstract: Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex.
Type:
Grant
Filed:
October 3, 2013
Date of Patent:
December 1, 2015
Assignee:
Great Basin Scientific Corporation
Inventors:
Robert D. Jenison, Joshua Klonoski, Anthony R. Torres
Abstract: Methods for rapidly detecting clinically relevant mutations in the infectious genome of an agent are disclosed. The methods include use of a novel target and temperature dependent RNase H mediated cleavage of blocked DNA primers to initiate isothermal helicase-dependent amplification of a target sequence such as a sequence in the rpoB gene.
Type:
Grant
Filed:
June 3, 2013
Date of Patent:
January 20, 2015
Assignee:
Great Basin Scientific Corporation
Inventors:
Brian Hicke, Robert D. Jenison, Madeline West