Abstract: A method of separating a saccharide from an aqueous product solution of the cellulose hydrolysis process is provided. The aqueous product solution comprises a saccharide and a cellulose swelling agent. The cellulose swelling agent is zinc chloride, magnesium chloride or a combination thereof. The method comprises the following steps in the given order: (a) adding a first tertiary amine and an optional first organic solvent to the aqueous product solution to provide a mixture; (b) performing a solid-liquid separation to obtain a solution from the mixture; and (c) performing a liquid-liquid extraction by adding a second tertiary amine and a second organic solvent to the solution, and then removing the organic phase and collecting the aqueous phase, wherein the first tertiary amine and the second tertiary amine are the same or different, and the first organic solvent and the second organic solvent are the same or different.

Abstract: A method of producing butyric acid, comprising: (a) providing a microorganism co-culture system comprising an air-tight container and the following (1) to (3) contained in the air-tight container: (1) a substrate, comprising a saccharide; (2) at least one of a first strain and a second strain, wherein the first strain is able to fix a carbon oxide and the second strain is able to fermentatively metabolize an amino acid, and wherein the first strain produces a first metabolite in the fermentation, the second strain produces a second metabolite in the fermentation, and each of the first metabolite and the second metabolite comprises acetic acid; and (3) a third strain, being able to metabolize the saccharide, the first metabolite and the second metabolite in the fermentation to produce butyric acid and a metabolic byproduct in fermentation, wherein the metabolic byproduct comprises carbon oxide and hydrogen, wherein, when the second strain is present in the co-culture system, the substrate further comprises

Abstract: An isolated Clostridium cadaveris ITRI04005 and its uses are provided. The isolated Clostridium cadaveris ITRI04005 was deposited at German Collection of Microorganisms and Cell Cultures (Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH, DSMZ) under the accession number DSM 32078.

Abstract: A microorganism capable of fixing a carbon oxide and performing fermentation and its preparation method and use are provided, wherein the preparation method comprises: (a) providing a first parental strain which is able to fix a carbon oxide; (b) providing a second parental strain which is able to perform fermentation; (c) preparing a first protoplast and a second protoplast from the first parental strain and the second parental strain, respectively; (d) subjecting the first protoplast and the second protoplast to fuse to provide a fusant; and (e) incubating and selecting the fusant to obtain a target strain. The target strain is useful in the production of an organic compound such as an organic acid and an alcohol, and one embodiment of the target strain is Clostridium tyrobutyricum ITRI02001, deposited at German Collection of Microorganisms and Cell Cultures (Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH, DSMZ) under the accession number DSM 32077.

Abstract: An isolated biologically pure culture of Clostridium tyrobutyricum ITRI04001 or an isolated biologically pure culture of Clostridium tyrobutyricum having the genotypic characteristics of ITRI04001 useful for syngas fermentation. Volatile free acids are produced by a method comprising culturing a microorganism having the genotypic characteristics of ITRI04001 in a medium; providing at least one substrate comprising at least one carbon source chosen from CO and CO2 to the microorganism; and recovering at least one free volatile free acid. Syngas can be the at least one substrate in these processes for producing volatile free acids.