Abstract: The mutant chorismate-pyruvate lyase (A) or (B) as described below is capable of producing 4-hydroxybenzoic acid or a salt thereof with sufficient practical efficiency. (A) A mutant chorismate-pyruvate lyase obtained by replacing the valine at position 80 in a chorismate-pyruvate lyase (ubiC) from Pantoea ananatis consisting of the amino acid sequence of SEQ ID NO: 1 with one or more other amino acids. (B) A mutant chorismate-pyruvate lyase obtained by replacing an amino acid in another chorismate-pyruvate lyase, the amino acid being at a position enzymologically homologous with that of the above valine, with one or mere other amino acids.
Abstract: The mutant chorismate-pyruvate lyase (A) or (B) as described below is capable of producing 4-hydroxybenzoic acid or a salt thereof with sufficient practical efficiency. (A) A mutant chorismate-pyruvate lyase obtained by replacing the valine at position 80 in a chorismate-pyruvate lyase (ubiC) from Pantoea ananatis consisting of the amino acid sequence of SEQ ID NO: 1 with one or more other amino acids. (B) A mutant chorismate-pyruvate lyase obtained by replacing an amino acid in another chorismate-pyruvate lyase, the amino acid being at a position enzymologically homologous with that of the above valine, with one or mere other amino acids.
Abstract: The mutant chorismate-pyruvate lyase (A) or (B) as described below is capable of producing 4-hydroxybenzoic acid or a salt thereof with sufficient practical efficiency. (A) A mutant chorismate-pyruvate lyase obtained by replacing the valine at position 80 in a chorismate-pyruvate lyase (ubiC) from Pantoea ananatis consisting of the amino acid sequence of SEQ ID NO: 1 with one or more other amino acids. (B) A mutant chorismate-pyruvate lyase obtained by replacing an amino acid in another chorismate-pyruvate lyase, the amino acid being at a position enzymologically homologous with that of the above valine, with one or more other amino acids.
Abstract: A transformant constructed by introducing a gene which encodes an enzyme having chorismate-pyruvate lyase activity into a coryneform bacterium as a host is capable of efficiently producing 4-hydroxybenzoic acid or a salt thereof from a sugar. When the transformant is cultured under aerobic conditions where the transformant does not grow, 4-hydroxybenzoic acid or a salt thereof can be produced in a particularly efficient manner.
Abstract: An aspect of the present disclosure relates to a functional defoaming agent composition, and more particularly to an environmentally-friendly defoaming agent composition containing loess. In an aspect, a loess-based, environmentally-friendly functional defoaming agent includes: 1-20 wt % loess; 10-30 wt % silicone resin having a kinematic viscosity of 10-50,000 cst; 1-5 wt % thickening agent; 1-10 wt % natural surfactant; 1-10 wt % water-purifying agent; and 60-80 wt % water. In another aspect, a loess-based, environmentally friendly functional defoaming agent includes: 1-20 wt % inorganic mixture of loess with at least one selected from among elvan, tourmaline and germanium; 10-30 wt % silicone resin having a kinematic viscosity of 10-50,000 cst; 1-5 wt % thickening agent; 1-10 wt % natural surfactant; 1-10 wt % water-purifying agent; and 60-80 wt % water.
Abstract: An aspect of the present disclosure relates to a functional defoaming agent composition, and more particularly to an environmentally-friendly defoaming agent composition containing loess. In an aspect, a loess-based, environmentally-friendly functional defoaming agent includes: 1-20 wt % loess; 10-30 wt % silicone resin having a kinematic viscosity of 10-50,000 cst; 1-5 wt % thickening agent; 1-10 wt % natural surfactant; 1-10 wt % water-purifying agent; and 60-80 wt % water. In another aspect, a loess-based, environmentally friendly functional defoaming agent includes: 1-20 wt % inorganic mixture of loess with at least one selected from among elvan, tourmaline and germanium; 10-30 wt % silicone resin having a kinematic viscosity of 10-50,000 cst; 1-5 wt % thickening agent; 1-10 wt % natural surfactant; 1-10 wt % water-purifying agent; and 60-80 wt % water.