Patents Assigned to GSF-Forschungszentrum fur Umwelt und Gesundheit
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Patent number: 6251672Abstract: Mammalian cells which require contact with cell surface proteins for activation, differentiation and/or proliferation are cultured in contact with an immobilized mammalian cell, with an immobilized membrane vesicle from a mammalian cell or with immobilized membrane vesicles from at least two mammalian cell populations. The mammalian cell or membrane vesicle can be modified with a first partner of a biological binding pair, and immobilized on a solid carrier via a second partner of the biological binding pair. Culturing can be with a first immobilized membrane vesicle and subsequently with a second immobilized membrane vesicle. The membrane vesicle contains part of the natural surface of a mammalian cell that includes a signal triggering surface protein. The membrane vesicle can be immobilized via a binding partner of a cell surface molecule.Type: GrantFiled: November 23, 1998Date of Patent: June 26, 2001Assignee: GSF-Forschungszentrum fur Umwelt und GesundheitInventors: Manfred Kubbies, Peter Dörmer, Petra Meissner
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Patent number: 6210668Abstract: The present invention discloses a procedure for the destruction of contaminating tumor cells in stem cell transplants ex vivo using intact bispecific antibodies.Type: GrantFiled: October 21, 1999Date of Patent: April 3, 2001Assignee: GSF Forschungszentrum fur Umwelt und Gesundheit GmbHInventors: Horst Lindhofer, Helge Menzel, Hans-Jochem Kolb, Stefan Thierfelder
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Patent number: 6210959Abstract: For avoidance of cell aggregation and damage to the cells as well as for a reduction of the risk of contamination, an apparatus for the cultivation and concentration of non-adherent cells as well as for the co-culture of two different cell species consists of an elongate hollow vessel comprising a resting surface extending in parallel with the longitudinal axis of said vessel for positioning the hollow vessel for cell culture operation, a standing surface provided orthogonally on the longitudinal axis for positioning the hollow vessel for cell-harvesting operation or for the co-culture of two cell species, at least one closable charging and discharging opening formed on the hollow vessel on the section of the latter which is opposite to said standing surface, and a partitioning wall extending inside the hollow vessel from the standing surface thereof, which partly subdivides the interior space of the vessel, with this partitioning wall being formed at least in parts with a liquid-permeable, however cell-imperType: GrantFiled: June 2, 1999Date of Patent: April 3, 2001Assignee: GSF-Forschungszentrum für Umwelt und Gesundheit GmbHInventors: Antal Lodri, Jean-Pierre Kremer, Gilbert Reisbach, Guoquing Liu
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Patent number: 6194205Abstract: T cells having a desired antigen specificity are stimulated by (a) introducing immortalizing genes into antigen-presenting cells in a manner permitting regulation of the expression and/or function of at least one of these genes to achieve conditionally immortalized antigen-presenting cells; (b) introducing a gene encoding the desired antigen into the immortalized cells in a manner permitting the antigen to be expressed after the expression and/or abolishment of the function of at least one of the immortalizing genes stops; (c) expanding the immortalized antigen-presenting cells by expression and/or functional activation of the immortalizing genes; (d) completing the proliferation of the immortalized antigen-presenting cells by stopping the expression and/or abolishing the function of at least one of the controllable immortalizing genes; (e) continuing the expression of the antigen; (f) adding leucocytic cells including T cells and cultivating the cell mixture to stimulate the T cells directed against the desiType: GrantFiled: September 14, 1998Date of Patent: February 27, 2001Assignee: GSF-Forschungszentrum fur Umwelt und Gesundheit GmbHInventors: Martin Staege, Bettina Kempkes, Georg W. Bornkamm, Wolfgang Hammerschmidt, Ursula Zimber-Strobl, Axel Polack
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Patent number: 6133198Abstract: A method of treating plants to raise their resistance to viroids and to protect them from the effects of viroid infections by administering bioassimilable boron compounds to the plants.Type: GrantFiled: December 19, 1998Date of Patent: October 17, 2000Assignee: GSF Forschungszentrum fur Umwelt und Gesundheit GmbHInventors: Eberhard Bengsch, Antonius Kettrup, Jurgen Polster
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Patent number: 6097033Abstract: In a dosimeter probe comprising a housing having two straps and including a cylindrical recess with a central extension a disc-like enclosure with an annular projection forming a circular cavity is disposed and a thermoluminescence detector is received in the cavity. At the open end of the cylindrical recess, the housing has an inwardly projecting annular flange structure which retains the enclosure in the recess and the enclosure is chamfered at the circumference of its disc portion for centering the enclosure with respect to the housing and facilitating insertion into the housing with a fit which provides for light-tight engagement between the enclosure and the housing walls.Type: GrantFiled: January 25, 1999Date of Patent: August 1, 2000Assignee: GSF-Forschungszentrum fur Umwelt und Gesundheit GmbHInventors: Hans-Norbert Brand, Gunter Krehbiel
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Patent number: 6007807Abstract: A hybrid fusion cell comprising:a) a malignant B cell which expresses an idiotypic antibody; andb) a hybridoma which expresses an antibody which is capable of being internalized on a professional antigen presenting cell.Type: GrantFiled: August 25, 1997Date of Patent: December 28, 1999Assignee: GSF Forschungszentrum fur Umwelt und Gesundheit GmbHInventors: Ralf Mocikat, Horst Lindhofer, Stefan Thierfelder
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Patent number: 5985276Abstract: The present invention discloses a procedure for the destruction of contaminating tumor cells in stem cell transplants ex vivo using intact bispecific antibodies.Type: GrantFiled: September 3, 1997Date of Patent: November 16, 1999Assignee: GSF Forschungszentrum fur Umwelt und Gesundheit GmbHInventors: Horst Lindhofer, Helge Menzel, Hans-Jochem Kolb, Stefan Thierfelder
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Patent number: 5901662Abstract: In order to fulfill the requirements in terms of biological compatibility and mechanical stability, a transponder-holding case is provided as implant in animal breeding and keeping, which case consists of a first case section and a second case section made of a biologically compatible synthetic material, with the two case sections being designed for being detachably fastened at one another, preferably through a self-sealing and self-locking threaded joint, so as to form a hermetically sealed holding space for a transponder to be received therein. For an improvement of the position-retaining function in the animal body at least one of the case sections presents at least one formed-in portion on ist outside, and at least on the outside of one case section a receiving area is provided for a preferably laser-engraved identifier mark.Type: GrantFiled: July 18, 1997Date of Patent: May 11, 1999Assignee: GSF-Forschungszentrum fur Umwelt und Gesundheit GmbHInventor: Georg Menache
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Patent number: 5849567Abstract: In a method for reducing the content of heavy metals of solid materials, particularly of soils, a 0.1-10% aqueous solution of chemically modified biomasses of waste and residue materials is applied to the solid material whereby the heavy metals are released and extracted from the solid material. The biomass solution residues remaining in the solid material after the heavy metal extraction procedure is rapidly biodegradable so that no objectionable traces remain in the solid materials or soils.Type: GrantFiled: April 28, 1997Date of Patent: December 15, 1998Assignee: GSF-Forschungszentrum fur Umwelt und Gesundheit GmbHInventors: Klaus Fischer, Hans-Peter Bipp, Peter Riemschneider, Antonius Kettrup, Dieter Bieniek
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Patent number: 5830473Abstract: A pharmaceutical kit and process useful for achieving prolonged immunosuppression and tumor cell elimination, wherein the kit comprises a first antibody having binding specificity for T cells and is capable of eliminating T cells in vivo; and a second antibody, having binding specificity for T cells and is capable of eliminating T cells in vivo, capable of modulating the antigen effect of T cells or both, wherein said first antibody differs from said second antibody in the constant region of its heavy chains and thus belongs to a different animal species, wherein said first antibody and said second antibody are maintained separately in said kit, and wherein in said process said first antibody is first applied once or several times and said second antibody is applied at a different time from said first antibody.Type: GrantFiled: May 7, 1997Date of Patent: November 3, 1998Assignee: GSF - Forschungszentrum Fur Umwelt Und Gesundheit GmbHInventor: Stefan Thierfelder
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Patent number: 5798230Abstract: The present invention provides a process for the preparation of human monoclonal antibodies from human B cells. According to this process, human antibody-producing B cells are immortalized by infection with an Epstein-Barr virus, or derivative thereof, that expresses Epstein-Barr virus nuclear antigen 2 (EBNA2). EBNA2 function, which is required for immortalization, is subsequently shut off, which results in an increase in antibody production. The invention provides a variety of systems that allow expression of EBNA2 function to achieve immortalization, followed by reduction of EBNA2 expression or function to facilitate antibody production.Type: GrantFiled: April 3, 1996Date of Patent: August 25, 1998Assignee: GSF-Forschungszentrum fur Umwelt und Gesundheit GmbHInventors: Georg Wilhelm Bornkamm, Dirk Eick, Bettina Kempkes, Nicola Maria Jochner, Lothar J. Strobl
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Patent number: 5770688Abstract: A method and composition are described for the treatment of mammalian HIV infections including administering an effective subtoxic dosage of melittin (Seq. ID NO:1) to the mammal whereby the growth of HIV infected cells or the replication of the virus in the infected cells of the mammal is inhibited.Type: GrantFiled: June 6, 1995Date of Patent: June 23, 1998Assignee: GSF-Forschungszentrum fur Umwelt und Gesundheit GmbHInventors: Torben Saermark, Volker Erfle
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Patent number: 5658761Abstract: A human bone marrow stromal cell line, which is characterized in that the cells of the cell line, after irradiation which results in the growth being arrested, remain adherent, is suitable for use as a feeder layer for supporting the proliferation of blood cells.Type: GrantFiled: January 11, 1996Date of Patent: August 19, 1997Assignee: Gsf-Forschungszentrum fur Umwelt und Gesundheit GmbHInventors: Karin Thalmeier, Peter Dormer
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Patent number: 5580297Abstract: In a method of extracting royal jelly from comb cells of queens, workers, and drones of Apis mellifera, the royal jelly is extracted under an inert gas cover such that it does not come into contact with air in the process, whereby the properties of the royal jelly are consistently retained.Type: GrantFiled: March 1, 1995Date of Patent: December 3, 1996Assignee: GSF-Forschungszentrum fur Umwelt und Gesundheit GmbHInventor: Eberhard Bengsch