Abstract: Transcription factor responsive elements, target genes, and co-factors of a transcription factor, either individually or in combination, are identified by integrating into the genome of a eukaryotic cell a gene trap vector that includes a reporter gene, a polyadenylation site, and a selectable marker gene, selecting the cells in which the gene trap vector is successfully integrated and contacting those cells with the transcription factor, then identifying and cultivating those cells having a gene into which the gene trap vector has been integrated and in which the reporter gene activity has been altered by interaction between the transcription factor and a transcription factor responsive element of that gene, and identifying the transcription factor responsive element, target gene, or cofactor that interacts with the transcription factor.

Abstract: In a gas inlet structure for an ion source, including a capillary for the admission of a sample gas, which capillary is disposed in a guide tube for discharging a sample gas into the guide tube, the guide tube has an open end disposed in the ion source. The guide tube includes a valve for the pulsed admission of a carrier gas to the guide tube. The guide tube, the valve and the capillary are supported in a sealed support housing from which the guide tube with the capillary disposed therein projects into the ion source for supplying thereto the sample gas in a pulsed manner.

Abstract: A device for administration of a medicated aerosol via the lungs consists of an inhalation mouthpiece 11, with an associated adjustable vaporizer 12, and of a compressed-air control valve 14 through which a pre-settable (13) volumetric flow of compressed air can be discharged to the vaporizer 12 containing the liquid medicament throughout a settable period of time. For operation of the device an electronic controller is provided on which the vaporizing period of the vaporizer and a pause interval can be set, with provisions being made for triggering the beginning of the vaporizing operation by means of a pressure sensor responsive to a suction pressure in the mouthpiece.

Abstract: Multiple myeloma is treated by deletion mutants of the gp130 protein of the IL-3 receptor. These mutants inhibit the binding of Hck tyrosine kinase and the growth of tumor cells, particularly myeloma cells.

Abstract: According to the invention, a method is provided wherein intact bispecific or trispecific antibodies which at the same time bind to the T cell receptor complex of a T cell, to tumor-associated antigens on a tumor cell, and, via the Fc portion of the bispecific antibody, to Fc receptor-positive cells are used for the induction of an anti-tumour immunity in humans and animals.

Abstract: In a device for separating the particle size spectrum of a polydisperse aerosol comprising a housing including an inlet part, a guide part, an outlet part, a nozzle insert and a separation insert, all parts are axially symmetrical and joined by centering fits so that they are easy to manufacture and can easily be assembled and also replaced. The parts are so fitted together that they are automatically centered during assembly while providing for the properly sized aerosol and air supply and particle fraction extraction channels.

Abstract: The present invention is directed to monoclonal antibodies capable of specifically binding to and recognizing an antigenic determinant (epitope) of the protein kaposin or a derivative thereof, hybridoma cell lines producing said monoclonal antibodies, diagnostic systems for the detection of the presence of a kaposin protein or a derivative thereof as well as antibodies directed against the kaposin protein or a derivative thereof, methods for detection of the expression of kaposin protein or a derivative thereof in a biological sample, methods for the detection of antibodies directed against kaposin protein or a derivative thereof, uses of the monoclonal antibodies provided according to the invention and uses of the kaposin protein or a derivative thereof, each in diagnostics and therapy.

Abstract: The present invention describes monoclonal antibodies which are useful for the specific detection and therapy of diffuse gastric carcinoma. Further embodiments describe therapeutic and diagnostic means for the detection and for the therapy of diffuse gastric carcinomas as well as methods for the detection and therapy of diffuse gastric carcinomas.

Abstract: The present invention relates to recombinant vaccinia viruses derived from the modified vaccinia virus Ankara (MVA) and containing and capable of expressing foreign genes which are inserted at the site of a naturally occurring deletion in the MVA genome, and the use of such recombinant MVA viruses for the production of polypeptides, e.g. antigens or therapeutic agents, or viral vectors for gene therapy, and the use of such recombinant MVA viruses encoding antigens as vaccines.

Abstract: Compositions of matter and methods for expressing a polypeptide are provided. In one aspect, a gene construct is provided that comprises an enhancer comprising particular elements or combinations of elements from the immunoglobulin &kgr; locus, the immunoglobulin heavy chain &mgr; locus, and the immunoglobulin &lgr; locus, and a promoter and polyadenylation site linked to a nucleotide sequence encoding a polypeptide from a select group. Cells comprising the gene construct, processes for producing such cells, and processes for producing the polypeptide encoded by the gene construct from such cells are also provided.

Abstract: The present invention provides a monoclonal or polyclonal antibody which specifically binds the Pax9 antigen as well as methods for using the same.

Abstract: The present invention is directed to monoclonal antibodies capable of specifically binding to and recognizing an antigenic determinant (epitope) of the protein kaposin or a derivative therof, hybridoma cell lines producing said monoclonal antibodies, diagnostic systems for the detection of the presence of a kaposin protein or a derivative thereof as well as antibodies directed against the kaposin protein or a derivative thereof, methods for detection of the expression of kaposin protein or a derivative thereof in a biological sample, methods for the detection of antibodies directed against kaposin protein or a derivative thereof, uses of the monoclonal antibodies provided according to the invention and uses of the kaposin protein or a derivative thereof, each in diagnostics and therapy.

Abstract: In a method of and a device for providing a predetermined constant medicament dose for an inhalational administration at a low inhalation flow rate, a closed container 11 is provided which is reducible in terms of volume and comprises a mouthpiece 12 for receiving a predetermined volume of a quantity of medicated aerosol, which is preferably inhalable in one breath, wherein the container 11 is compressed under air-flow control in a direction towards the mouthpiece 12 for preparation of the filling operation, subsequently a means for powder aerosol production is connected to the mouthpiece 12, and finally the container is filled at a high flow rate by air-flow controlled expansion of the container for the introduction of air through the powder aerosol generator.

Abstract: The present invention describes monoclonal antibodies which are useful for the specific detection of diffuse gastric carcinoma. Further embodiments describe therapeutic and diagnostic means for the detection and for the therapy of diffuse gastric carcinomas.

Abstract: The present invention relates to recombinant vaccinia viruses derived from the modified vaccinia virus Ankara (MVA) and containing and capable of expressing foreign genes which are inserted at the site of a naturally occurring deletion in the MVA genome, and the use of such recombinant MVA viruses for the production of polypeptides, e.g. antigens or therapeutic agents, or viral vectors for gene therapy, and the use of such recombinant MVA viruses encoding antigens as vaccines.

Abstract: The present invention relates to a method for helper virus-free packaging of gene vector DNA into the virus particles of a DNA virus as well as to eukaryotic helper cells for helper virus-free packaging of gene vector DNA into the virus particles of a DNA helper virus wherein a DNA virus having a genome ≧100 kbp is employed (FIG. 1).

Abstract: In a method and apparatus for determining neutron spectra using at least two neutron detectors which provide integral counting rates from which the spectrum of a neutron radiation can be approximated, and which consist each of a semiconductor diode, a converter layer, an inactive layer and an active layer. The various layers of each detector are different from those of the other neutron detector and so selected that the sensitivity functions of the two neutron detectors are different. An artificial neutral network is provided which is especially trained and to which the counting rates of the detectors are supplied to be processed for obtaining the neutron spectrum.

Abstract: The invention relates to a device for controlled inhalational administration of controlled-dosage drugs into the lungs, comprising a closed recipient adapted to be charged with a predeterminable aerosol volume and from which the aerosol may be withdrawn by means of a control means for controlling the inhalant flow.

Abstract: The present invention is directed to a method of prognosticating the illness development of patients with carcinoma of the breast and/or for diagnosing carcinoma of the breast, the method comprising a qualitative determination of T1 protein and/or T1-mRNA in a sample material obtained from the patient. The invention further pertains to kits for performing the methods according to the invention.

Abstract: In a method for producing a directed gas jet wherein a guided sample gas beam is generated and an auxiliary gas beam is generated and directed and guided in the same direction as, but separated from, the sample gas beam, a pulsed carrier gas stream is generated and combined with the sample gas beam such that the sample gas beam is separated into spaced pulses which are embedded between the axially spaced pulses of the carrier gas beam and the carrier gas beam with the sample gas beam embedded therein is combined with the auxiliary gas beam such that the carrier and sample gas beam is radially enveloped by the auxiliary gas beam to from the directed gas jet of a carrier and sample gas pulses enveloped in the auxiliary gas beam.