Abstract: The present disclosure provides a Brillouin optical time domain reflectometer with an ultra-high spatial resolution based on bipolar differential phase encoding, including a narrow-linewidth laser, a polarization maintaining coupler, a differential encoder, a Mach-Zehnder modulator, an optical pulse amplifier, an optical circulator and a sensing fiber, an optical amplifier, a coherent optoelectronic receiver, a sideband demultiplexer, and a result measurement module. By implementing the present disclosure, a spatial resolution for measuring a Brillouin scattering spectrum can be effectively improved, and measurement precision can be effectively improved by increasing a quantity of times of superposition and averaging. In addition, measurement performance can be effectively improved by directly using a bipolar encoding sequence.