Abstract: The present invention discloses a primer combination for detecting 9 NA subtype AIVs and an application thereof. The present invention protects a primer combination comprising 10 primer pairs (20 primers), and the 20 primers are set forth in SEQ ID NO: 1 to SEQ ID NO: 20 of the Sequence Listing in sequence. When used for identifying 9 NA subtypes, a GeXP detection method in combination with the primer combination provided by the present invention has the advantages of high throughput, fast, time-saving and labor-saving. The present invention provides an effective method for rapid identification of 9 different NA subtype AIVs and has a broad application prospect.
Type:
Grant
Filed:
April 24, 2019
Date of Patent:
March 22, 2022
Assignee:
GUANGXI VETERINARY RESEARCH INSTITUTE
Inventors:
Zhixun Xie, Sisi Luo, Jiaoling Huang, Zhiqin Xie, Liji Xie, Minxiu Zhang, Meng Li, Sheng Wang, Dan Li, Tingting Zeng, Yanfang Zhang, Qing Fan, Xianwen Deng
Abstract: Provided herein are a GeXP rapid detection primer set for simultaneously identifying gene HA of eight different human-infected subtypes of avian influenza virus, a kit, and use thereof. Disclosed are 9 pairs of specific primer and 1 pair of universal primer, for a GeXP rapid detection primer kit for simultaneously identifying gene HA of eight different human-infected subtypes of avian influenza virus. Eight different human-infected subtypes HA of avian influenza virus from the nine genes of M, H1, H2, H3, H5, H6, H7, H9 and H10 can be identified simultaneously with a sensitivity of 102 copies/?L.
Type:
Grant
Filed:
November 28, 2016
Date of Patent:
September 17, 2019
Assignee:
Guangxi Veterinary Research Institute
Inventors:
Zhixun Xie, Meng Li, Zhiqin Xie, Sisi Luo, Liji Xie, Li Huang, Xianwen Deng, Jiaoling Huang, Qing Fan, Yanfang Zhang, Tingting Zeng, Sheng Wang
Abstract: The present disclosure discloses a primer combination and GeXP detection method for simultaneously identifying eight kinds of bovine pathogens. The primer combination of the present disclosure consists of primer pair I, primer pair II, primer pair III, primer pair IV, primer pair, primer pair VI, primer pair VII and primer pair VIII. The present disclosure also discloses a GeXP detection method that can simultaneously identify bovine infectious diseases of foot-and-mouth disease virus, bluetongue virus, vesicular stomatitis virus, bovine viral diarrhea virus, bovine rotavirus, enterotoxigenic E. coli, infectious bovine rhinotracheitis virus and peste des petits ruminants virus. The GeXP detection method established can simultaneously identify the eight pathogens of bovine infectious diseases.
Type:
Application
Filed:
April 19, 2017
Publication date:
October 11, 2018
Applicant:
GUANGXI VETERINARY RESEARCH INSTITUTE
Inventors:
Zhixun XIE, Qing FAN, Zhiqin XIE, Xianwen DENG, Liji XIE, Li HUANG, Sisi LUO, Jiaoling HUANG, Yanfang ZHANG, Tingting ZENG, Sheng WANG, Jiabo LIU, Yaoshan PANG
Abstract: Provided herein is a GeXP detection kit for identification of 11 kinds of duck virus diseases. The detection kit includes a primer set for identifying or auxiliarily identifying pathogens of duck communicable diseases, including one or more of primer pair A, primer pair B primer pair C, primer pair D, primer pair E, primer pair F, primer pair G, primer pair H, primer pair I, primer pair J, primer pair K and primer pair L. The kit can detect simultaneously avian influenza virus, subtype H5, H7 and H9 of avian influenza virus, duck hepatitis virus, duck enteritis virus, duck Tembusu virus, Newcastle disease virus, egg drop syndrome virus, Muscovy duck reovirus, Muscovy duck parvovirus and duck circovirus with the primer set, PCR reagent or primer pairs provided in the present invention.
Type:
Grant
Filed:
March 21, 2014
Date of Patent:
May 2, 2017
Assignee:
Guangxi Veterinary Research Institute
Inventors:
Zhixun Xie, Yanfang Zhang, Liji Xie, Jiabo Liu, Qing Fan, Sisi Luo, Xianwen Deng, Zhiqin Xie, Yaoshan Pang