Patents Assigned to Guest Elchrom Scientific
  • Patent number: 6017436
    Abstract: A gel for electrophoresis in a horizontal mode which contains at least one sample well with an enlarged loading area that is formed in an elevated gel segment. At least a front wall of the sample well consists of a substantially vertical part and a non-vertical part, where the non-vertical part is declined at an angle sufficiently steep that the sample loaded on the non-vertical part slides down into the sample well. Any sample molecules that during electrophoresis enter the gel through the non vertical part of the front wall migrate only through the elevated gel segment. They also exit this segment, so that substantially no double bands, which could complicate the interpretation of experimental results, are detected.
    Type: Grant
    Filed: June 16, 1998
    Date of Patent: January 25, 2000
    Assignee: Guest Elchrom Scientific AG
    Inventor: Branko Kozulic
  • Patent number: 5840877
    Abstract: Electrophoresis gels of enhanced selectivity are produced by adding a preformed polymer to a polymerization solution containing at least one monomer and at least one cross-linker. The polymer must be of such a chemical composition and molecular weight that its presence during free-radical polymerization results in a new topology of gel polymers, so that under the influence of an electric field, such as during electrophoresis, the passage of long DNA molecules through the gel is retarded proportionally more than the passage of the short ones. The polymer needs to be added at specific ratio to the monomer and the cross-linker, and the enhanced selectivity is achievable only within a certain range of ratios of polymer(s), monomer(s) and cross linker(s). That range is dependent on all three essential gel components, the polymer, the monomer and the cross-linker.
    Type: Grant
    Filed: September 5, 1996
    Date of Patent: November 24, 1998
    Assignee: Guest Elchrom Scientific
    Inventor: Branko Kozulic
  • Patent number: 5800691
    Abstract: A gel for electrophoresis in a horizontal mode which contains at least one sample well with an enlarged loading area that is formed in an elevated gel segment. At least a front wall of the sample well consists of a substantially vertical part and a non-vertical part, where the non-vertical part is declined at an angle sufficiently steep that the sample loaded on the non-vertical part slides down into the sample well. Any sample molecules that during electrophoresis enter the gel through the non-vertical part of the front wall migrate only through the elevated gel segment. They also exit this segment, so that substantially no double bands, which could complicate the interpretation of experimental results, are detected.
    Type: Grant
    Filed: December 23, 1996
    Date of Patent: September 1, 1998
    Assignee: Guest Elchrom Scientific AG
    Inventor: Branko Kozulic
  • Patent number: 5541255
    Abstract: A bed of water insoluble, transparent, cross-linked gel, suitable for use in electrophoresis, is formed by dissolving a polysaccharide, including at least one linear polysaccharide such as agarose or hydroxyethyl cellulose, in a suitable solvent, such as water; adding a cross-linking agent, which is not charged nor becomes charged upon contact with water in a pH range of 2 to 11, to the solution; and incubating this mixture in a quiescent state to substantially simultaneously react the polysaccharide and the cross-linking agent and to gel the reaction product into a bed. The polysaccharide is at least one linear polysaccharides, but that linear polysaccharide may also be admixed with other linear polysaccharides and/or at least one non-linear polysaccharide. Synthetic organic polymers may also be admixed in the cross-linking reaction mixture.
    Type: Grant
    Filed: June 30, 1994
    Date of Patent: July 30, 1996
    Assignee: Guest Elchrom Scientific, Ltd.
    Inventor: Branko Kozulic
  • Patent number: 5371208
    Abstract: A bed of transparent, water insoluble, charge-free crosslinked gel which is suitable for use in electrophoresis is prepared. The gel is formed by dissolving a linear polysaccharide, such as agarose, in a suitable solvent, such as water. This dissolution may be accomplished with agitation. A suitable cross linking agent, which is not itself charged, nor does it become charged upon contact with water, at a pH of 2 to 12, is then added to the solution, possibly with agitation in order to get thorough mixing of the linear polysaccharide and the cross linking agent. This mixture is then allowed to incubate and react in a quiescent state to substantially simultaneously gel and crosslink to form the desired cross linked gel bed. Preferred cross-linking agents are bis-epoxides, halo-epoxides, bis-haloalkanes, bis-halo-alcohols, alkanediol-bis-alkyl sulfonates, alkanediol-bis-aryl sulfonates or divinylsulfone. The liner polysaccharide maybe mixed with a synthetic polymer such as polyvinyl alcohol.
    Type: Grant
    Filed: December 30, 1992
    Date of Patent: December 6, 1994
    Assignee: Guest Elchrom Scientific Ltd.
    Inventor: Branko Kozulic