Abstract: Spectrophotometric apparatus and methodology suitable for continuous and long-term use. The apparatus includes a monochromator providing pre-dispersed monochromatic light to the optical inputs of a pair of fiber optic cables and a translator for alternatively positioning the fiber optic cables at the same location with respect to the monochromator output. One of the cables conducts light to a sample under study while the other cable provides a reference for light intensity measurements. The methodology includes the steps of performing two scans through the monochromator output for each measurement on the sample. The fiber optic cables are moved between scans so that the cable employed in the latter scan occupies the former position of the cable employed in prior scan.