Abstract: The present invention relates to an eco-friendly probiotic composition, and more particularly, to a probiotic composition for livestock production, comprising a lactic acid bacteria culture medium and an organic acid, wherein the organic acid is contained in an amount of 0.002 to 0.1 parts by weight relative to 1 parts by weight of the lactic acid bacteria culture medium. The present invention also relates to a method for preparing the composition. The eco-friendly probiotic composition of the present invention does not contain antibiotics and may exhibit complex effect of sterilization and virucidal effectiveness.