Abstract: A microfluidic disc having one or more enclosed microchannel structures, and the microchannel structures are intended to be used for transport of transporting liquids. The device is characterized in that at least a part of the inner walls of each of one or more microchannel structures are treated with a gas plasma having one or more organic precursor compounds.

Abstract: A microfluidic device that comprises several microchannel structures in which there are an inlet port, an outlet port and there between a structural unit comprising a fluidic function. The structural unit can be selected amongst units enabling a) retaining of nl-aliquots comprising constituents which has been defined by mixing of aliquots within the microfluidic device (unit A), b) mixing of aliquots of liquids (unit B), c) partition of larger aliquots of liquids into smaller aliquots of liquids and distributing the latter individually and in parallel to different microchannel structure of the same microfluidic device (unit C), d) quick penetration into a microchannel structure of an aliquot of a liquid dispensed to an inlet port of a microchannel structure (unit D), and e) volume definition integrated within a microchannel structure (unit E).

Abstract: Disclosed is a method for performing the steps of nucleic acid template purification, a thermocycling reaction and purification of the products of the thermocycling reaction characterised in that the steps take place sequentially in a microfluidic disc. Also disclosed is a microstructure for fluids comprising at least one inlet opening connected to a first chamber incorporating a means for purifying template nucleic acid which, in turn, is connected to a second chamber incorporating a means for a thermocycling reaction which, in turn, is connected to a third chamber incorporating a means for purifying products of the thermocycling reaction, and a microfluidic disc comprising a plurality of such microstructures.

Abstract: The invention relates to a matrix suitable for use in the replication of a plastic element having a positive microstructure, comprising a first wear-resistant layer that is supported by a carrier element, wherein the matrix comprises a heating means for supplying electrical heat energy through the wear-resistant layer or carrier element. The invention further relates to a plastic element producing machine and a method for the manufacture of a plastic element having a surface with a positive microstructure.

Abstract: A method for dispensing droplets of a liquid to a microsystem in the form of a disc comprising a target area (TA0I) in its surface, said disc preferably being a microfluidic disc comprising a microchannel structure with an inlet port that is a target area (TA0I). The method is characterized by comprising the steps of: i) providing (1) said disc which has a triggering mark, and (2) a dispenser arrangement comprising: a) a spinner for rotating the disc around its axis, b) a drop dispenser permitting dispensation of droplets to inlet port I, c) a fixed trigger position outside the disc, and d) a controller which is capable of triggering the dispensation of a droplet into (TA0I) as a function of the triggering mark passing the trigger position, ii) placing the disc in the spinner and programming the controller with values for dispensing parameters that will give dispensation of the droplets to TA0I, iii) dispensing the droplets. The invention also comprises the dispenser arrangement as such.

Abstract: Disclosed is a method and apparatus for the isolation of DNA or cell nuclei or a mixture thereof from cell samples in a CD device. The method includes treating a suspension of whole cells with a lysis reagent so as to lyse the cytoplasmic membrane and at least some of the nuclear membranes, and introducing the lysate into micro-channels of a microfabricated apparatus in which each of the micro-channels is provided with a barrier disposed in the channel to impede the passage or flow of DNA and cell nuclei each while allowing the passage of liquid through the micro-channel so that a mesh comprising DNA is formed in the channel.

Abstract: The present invention relates to a method for presenting an analyte of a liquid sample as an MS-analyte to a mass spectrometer. More particularly, the method comprises the steps of applying a liquid sample containing the analyte to a sample inlet port of a microchannel structure of a microfluidic device, said structure also comprising an outlet port (MS-port) that is capable of being interfaced with a mass spectrometer, passing the analyte to the MS-port thereby transforming it to an MS-analyte, and presenting the MS-analyte to mass spectrometer via the MS-port.

Abstract: A microfluidic device in form of a disc comprising an MS-port for presentation of an MS-analyte to an EDI-MS apparatus, said MS-port is a part of a microchannel structure (I) comprising an inlet port for a sample, and comprises an EDI-area having a conductive layer (I) and an EDI-surface from which the MS-analyte is to be desorbed/ionised. The device is characterized in that layer (I) has a conductive connection and/or that there is a calibrator area in the proximity of each of said one, two or more MS-ports. In a typical variant the MS-port is in the form of a depression that is in fluid communication with upstream part of microchannel structure (I).

Abstract: A method of producing a plastic product having a microstructured surface (3a), utilizing an arrangement for moulding plastic products. Said arrangement has at least two mould parts (2, 3) movable towards and away from each other, a predetermined volume of a viscous polymerizable plastic material (9) being supplied to a cavity formed between the mould parts, after which polymerization of the plastic material occurs. After said polymerization of the plastic material (9′), the mould parts are caused to move apart from each other thereby allowing removal of a finished plastic product the outer shape of which conforms to the shape of the cavity produced by the mould parts. Said viscous polymerizable plastic material (9) is supplied to the cavity (6) around a hole (10a) pertaining to the finished plastic product, and said viscous plastic material is distributed in a direction from said hole (10a) towards the peripheral edge area of the plastic product.

Abstract: The invention can be described as a Method Handler (MH), which consists of several parts used to check and create different methods for microfluidic instruments. The part that creates methods can be called the Method Editor (ME). It is used to create a scheme of operations that together with the instrument builds up an application for a specific disc type. MH also checks if a method is executable on a specific instrument. This is done in a part called Batch Run (BR).

Abstract: A microfluidic device comprising an MS-analyte presentation unit for a EDI-MS apparatus, said unit comprising an essentially planar support plate which on one side has one, two or more ports (MS-ports) comprising an area (EDI area) for presenting the MS-analyte to a mass spectrometer. The EDI area comprises a layer I of conducting material. The characteristic feature of the device is that layer (I) has a conductive connection and/or that there is a calibrator area in the proximity of the MS-port.

Abstract: The present invention relates to a method for presenting an analyte of a liquid sample as an MS-analyte to a mass spectrometer. More particularly, the method comprises the steps of applying a liquid sample containing the analyte to a sample inlet port of a microchannel structure of a microfluidic device, said structure also comprising an outlet port (MS-port) that is capable of being interfaced with a mass spectrometer, passing the analyte to the MS-port thereby transforming it to an MS-analyte, and presenting the MS-analyte to mass spectrometer via the MS-port.

Abstract: Apparatus and methods are provided for performing cell growth and cell based assays in a liquid medium. The apparatus comprises a base plate supporting a plurality of micro-channel elements, each micro-channel element comprising a cell growth chamber, an inlet channel for supplying liquid sample thereto and an outlet channel for removal of liquid sample therefrom, a cover plate positioned over the base plate to define the chambers and connecting channels, the cover plate being supplied with holes to provide access to the channels. Means are incorporated in the cell growth chambers, for cell attachment and cell growth. In particular, the invention provides a rotatable disc microfabricated for performing cell growth and cell based assays. The apparatus and method can be used for the growth of cells and the detection and measurement of a variety of biochemical processes and products using non-invasive techniques, that is techniques which do not compromise the integrity or viability of cells.

Abstract: The present invention relates to a circular disk, comprising two material layers that are joined together, wherein said disk contains microfluidic structures formed of a channel and/or cavity system defined between the two material layers, the recesses which correspond to the channels and/or cavities, respectively, being formed in one or both of opposed layer surfaces.

Abstract: The present invention concerns a detector arrangement that is adapted to measure radiation from detector areas in the surface of a microfluidic device. The arrangement comprises a detector unit with a detector head and other units enabling for the detector unit to collect radiation from the detection areas.

Abstract: A microfluidic device which comprises two or more microchannel structures (set 1), each of which comprises a structural unit which comprises (i) one or more inlet microconduits, and (ii) an outlet microconduit downstream said one or more inlet microconduits, and (iii) a flow path for a liquid passing through either of said inlet microconduits and said outlet microconduit. The device is characterized in that each outlet microconduit in said two or more microchannel structures is a restriction microconduit. There may also be a microcavity between the inlet microconduit(s) and the restriction microconduit in each microchannel structure. Typically common flow control is used for driving a liquid flow within the device. The innovative design is useful for creating flow with low inter-channel variation with respect to the microchannel structures of the device.

Abstract: A microfluidic device that comprises several microchannel structures in which there are an inlet port, an outlet port and there between a structural unit comprising a fluidic function. The structural unit can be selected amongst units enabling a) retaining of nl-aliquots comprising constituents which has been defined by mixing of aliquots within the microfluidic device (unit A), b) mixing of aliquots of liquids (unit B), c) partition of larger aliquots of liquids into smaller aliquots of liquids and distributing the latter individually and in parallel to different microchannel structure of the same microfluidic device (unit C), d) quick penetration into a microchannel structure of an aliquot of a liquid dispensed to an inlet port of a microchannel structure (unit D), and e) volume definition integrated within a microchannel structure (unit E).

Abstract: The invention relates to a method of producing a matrix suitable for use in a plastic element-producing machine, a matrix produced in accordance with the method and use of such a matrix to form a plastic element. One side of the matrix is provided with a negative microstructure which is replicated in plastic material to form a positive microstructure on a plastic element, wherewith the matrix can be produced by applying a layer of material to the positive microstructure side of a master. The positive microstructure side of the master has a thin wear-resistant layer which serves as a first wear surface, wherewith said layer will present irregularities which correspond essentially to the microstructure, and wherein at least said irregularities are filled-in with a plastic composite so as to form a carrier element for supporting the first wear layer. The layer can further be provided with means for supplying heat energy to said matrix.

Abstract: A method of capillary electrophoresis utilizing a capillary tube made up of hydrophobic plastics, charactarized in that the inner surface of said capillary is coated with a hydrophilic polymer comprising a polyhydroxy polymer exhibiting groups —B—R, where R is a hydrocarbyl group and B is a bridge binding to the polyhydroxy polymer, preferably by substituting a hydrogen in a hydroxy group of the polyhydroxy polymer.