Abstract: The present invention relatives to a method for manufacturing hydrogen-saturated deuterium-depleted water, comprising (a) providing a distilled or mineral water; (b) providing a hydrogen storage apparatus for providing a high purity hydrogen; (c) controlling a pressure of hydrogen gas between 3˜8 bar at a working environment temperature of 10˜28° C.; (d) controlling a flow velocity of hydrogen gas between 3˜5 L/min and inletting hydrogen into the distilled or mineral water to produce a pressure difference to replace deuterium from the distilled water; and (e) controlling a working time between 30˜90 mins to produce a hydrogen-saturated deuterium-depleted water. Therefore, a method for manufacturing hydrogen-saturated deuterium-depleted water with low consuming energy and low production cost is provided.