Abstract: The present disclosure relates to multifunctional verification molecules, including molecules according to formula (I): G-L-(B)K-Q-U, wherein G, L, B, K, Q, and U are defined herein. The present disclosure also relates to methods of preparing and using such multifunctional verification molecules to remove defective multifunctional molecules and to quantify synthetic yield.

Abstract: The present disclosure relates to multifunctional molecules, including molecules according to formula (I): ([(B1)M-D-L1]Y—H1)O-G-(H2-[L2-E-(B2)K]W)P, ??(I) wherein G, H1, H2, D, E, B1, B2, M, K, L1, L2, O, P, Y, and W are defined herein. The present disclosure also relates to methods of preparing and using such multifunctional molecules to identify encoded molecules capable of binding target molecules.

Abstract: The present disclosure provides methods and systems for determining a target-activity of at least one resolved oligonucleotide encoded molecule. In an embodiment, a method includes providing a separation medium, wherein the separation medium contains at least one target molecule; and various methods of separating a mixture of at least two oligonucleotide encoded molecules by electrophoresis based on different target-activities of the oligonucleotide encoded molecules for a target molecule. Benefits of the methods disclosed herein can include, without limitation, collecting and calculating qualitative and quantitative data for the target-activity of an encoded portion of the oligonucleotide encoded molecule for a target molecule.

Abstract: The present disclosure relates to multifunctional molecules, including molecules according to formula (I): ([(B1)M-D-L1]Y-H1)O-G-(H2-[L2-E-(B2)K]W)P, ??(I) wherein G, H1, H2, D, E, B1, B2, M, K, L1, L2, O, P, Y, and W are defined herein. The present disclosure also relates to methods of preparing and using such multifunctional molecules to identify encoded molecules capable of binding target molecules.

Abstract: The present disclosure relates to multifunctional molecules, including molecules according to formula (I-A) [(B1)M-L1]O-G, and (I) [(B1)M-L1]O-G-[(L2-(B2)K]P wherein B1, M, L1, O, G, L2, B2, K, and P are defined herein, wherein each positional building block B1 is identified by from 1 to 5 coding regions in G, and from about 10% to 100% of the positional building blocks B1 at position M and/or B2 at position K, based on a total number of positional building blocks, are identified by a combination of from 2 to 5 independent coding regions. Methods of making such multifunctional molecules, and methods of serially enriching an oligonucleotide encoded library, are also disclosed. The present disclosure also relates to methods of preparing and using such multifunctional molecules to identify encoded molecules capable of binding target molecules.