Abstract: Provided is a monoclonal antibody specific for hippuric acid. In the present invention, a hippuric acid-carrier protein conjugate is prepared from hippuric acid and BSA or OVA as a carrier protein, using a coupling reagent and a cross-linker. The monoclonal antibody screened according to the present invention has a titer having a standard curve in the concentration range of mg/mL meeting requirements for the permissible exposure limit (PEL) of toluene. The monoclonal antibody exhibits no cross-reactivity with carrier proteins, exhibits higher competitive inhibition in response to an increasing concentration of hippuric acid, and exhibits no cross-reactivity with other proteins contained in the urine. Therefore, the disclosed monoclonal antibody can be usefully employed in a diagnostic kit for detection of hippuric acid which is capable of diagnosing toluene exposure.
Type:
Grant
Filed:
July 30, 2005
Date of Patent:
October 19, 2010
Assignees:
HBI Co., Ltd.
Inventors:
Gun Sik Tae, Hee Sun Chung, Oh Hyep Kwon, Seung Hwa Lee