Abstract: The invention relates to a method for making a measurement substrate plate for the in vitro spectrophotometry testing of a cosmetic product including anti-UV filters, wherein said method comprises the following steps: using a mould with a textured bottom reproducing in a hollow form a predetermined topography; injecting into said mould a material that is transparent to UVA and UVB rays in order to form by moulding a preform of the measurement substrate plate; and releasing the measurement substrate plate thus obtained. The invention also relates to the measurement substrate plate obtained by said method.