Patents Assigned to Hemoshear, LLC
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Patent number: 11061016Abstract: The present invention generally relates to in vitro methods for mimicking in vivo pathological or physiologic conditions. The methods comprise applying shear forces to a cell type or cell type plated on a surface within a cell culture container. Methods for testing drugs or compounds in such systems are also described.Type: GrantFiled: December 18, 2019Date of Patent: July 13, 2021Assignee: HemoShear, LLCInventors: Brett R. Blackman, Brian R. Wamhoff, Ajit Dash, Michael B. Simmers, Ryan E. Feaver
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Patent number: 11008549Abstract: Methods for mimicking a tumor microenvironment in vitro are provided. The methods comprise indirectly applying a shear stress upon at least one tumor cell type plated on a surface within a cell culture container. Methods for mimicking tumor metastasis and methods for testing drugs or compounds in such systems are also provided.Type: GrantFiled: February 22, 2019Date of Patent: May 18, 2021Assignee: Hemoshear, LLCInventors: Brian R. Wamhoff, Brett R. Blackman, Robert A. Figler, Daniel G. Gioeli, Michael B. Simmers
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Patent number: 10837957Abstract: The present invention generally relates to in vitro methods for mimicking in vivo pathological or physiologic conditions. The methods comprise applying shear forces to a cell type or cell type plated on a surface within a cell culture container. Methods for testing drugs or compounds in such systems are also described.Type: GrantFiled: May 19, 2017Date of Patent: November 17, 2020Assignee: HemoShear, LLCInventors: Brett R. Blackman, Brian R. Wamhoff, Ajit Dash, Michael B. Simmers, Ryan E. Feaver
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Patent number: 10514374Abstract: The present invention generally relates to in vitro methods for mimicking in vivo pathological or physiologic conditions. The methods comprise applying shear forces to a cell type or cell type plated on a surface within a cell culture container. Methods for testing drugs or compounds in such systems are also described.Type: GrantFiled: November 18, 2016Date of Patent: December 24, 2019Assignee: HemoShear, LLCInventors: Brett R. Blackman, Brian R. Wamhoff, Ajit Dash, Michael B. Simmers, Ryan E. Feaver
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Patent number: 10221394Abstract: Methods for mimicking a tumor microenvironment in vitro are provided. The methods comprise indirectly applying a shear stress upon at least one tumor cell type plated on a surface within a cell culture container. Methods for mimicking tumor metastasis and methods for testing drugs or compounds in such systems are also provided.Type: GrantFiled: April 10, 2017Date of Patent: March 5, 2019Assignee: HemoShear, LLCInventors: Brian R. Wamhoff, Brett R. Blackman, Robert A. Figler, Daniel G. Gioeli, Michael B. Simmers
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Patent number: 9658211Abstract: The present invention generally relates to in vitro methods for mimicking in vivo pathological or physiologic conditions. The methods comprise applying shear forces to a cell type or cell type plated on a surface within a cell culture container. Methods for testing drugs or compounds in such systems are also described.Type: GrantFiled: April 18, 2013Date of Patent: May 23, 2017Assignee: HemoShear, LLCInventors: Brett R. Blackman, Brian R. Warnhoff, Ajit Dash, Michael B. Simmers, Ryan E. Feaver
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Patent number: 9617521Abstract: Methods for mimicking a tumor microenvironment in vitro are provided. The methods comprise indirectly applying a shear stress upon at least one tumor cell type plated on a surface within a cell culture container. Methods for mimicking tumor metastasis and methods for testing drugs or compounds in such systems are also provided.Type: GrantFiled: October 21, 2014Date of Patent: April 11, 2017Assignee: HemoShear, LLCInventors: Brian R. Wamhoff, Brett R. Blackman, Robert A. Figler, Daniel G. Gioeli, Michael B. Simmers
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Patent number: 9500642Abstract: The present invention generally relates to in vitro methods for mimicking in vivo pathological or physiologic conditions. The methods comprise applying shear forces to a cell type or cell type plated on a surface within a cell culture container. Methods for testing drugs or compounds in such systems are also described.Type: GrantFiled: April 18, 2013Date of Patent: November 22, 2016Assignee: Hemoshear, LLCInventors: Brett R. Blackman, Brian R. Wamhoff, Ajit Dash, Michael B. Simmers, Ryan E. Feaver
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Patent number: 8871461Abstract: Methods and devices for applying hemodynamic patterns to human/animal cells in culture are described. Hemodynamic flow patterns are measured directly from the human circulation and translated to a motor that controls the rotation of a cone. The cone is submerged in fluid (i.e., cell culture media) and brought into close proximity to the cells. Rotation of the cone creates time-varying shear stresses. This model closely mimics the physiological hemodynamic forces imparted on endothelial cells in vivo. A TRANSWELL coculture dish (i.e., a coculture dish comprising an artificial porous membrane) may be incorporated, permitting two, three, or more different cell types to be physically separated within the culture dish environment. In-flow and out-flow tubing may be used to supply media, drugs, etc. separately and independently to both the inner and outer chambers. The physical separation of the cell types permits each cell type to be separately isolated for analysis.Type: GrantFiled: October 11, 2010Date of Patent: October 28, 2014Assignee: Hemoshear, LLCInventors: Brett R. Blackman, Brian R. Wamhoff
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Publication number: 20130309677Abstract: The present invention generally relates to in vitro methods for mimicking in vivo pathological or physiologic conditions. The methods comprise applying shear forces to a cell type or cell type plated on a surface within a cell culture container. Methods for testing drugs or compounds in such systems are also described.Type: ApplicationFiled: April 18, 2013Publication date: November 21, 2013Applicant: HemoShear, LLCInventors: Brett R. Blackman, Brian R. Wamhoff, Ajit Dash, Michael B. Simmers, Ryan E. Feaver
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Publication number: 20110059480Abstract: Methods and devices for applying hemodynamic patterns to human/animal cells in culture are described. Hemodynamic flow patterns are measured directly from the human circulation and translated to a motor that controls the rotation of a cone. The cone is submerged in fluid (i.e., cell culture media) and brought into close proximity to the cells. Rotation of the cone creates time-varying shear stresses. This model closely mimics the physiological hemodynamic forces imparted on endothelial cells in vivo. A TRANSWELL coculture dish (i.e., a coculture dish comprising an artificial porous membrane) may be incorporated, permitting two, three, or more different cell types to be physically separated within the culture dish environment. In-flow and out-flow tubing may be used to supply media, drugs, etc. separately and independently to both the inner and outer chambers. The physical separation of the cell types permits each cell type to be separately isolated for analysis.Type: ApplicationFiled: October 11, 2010Publication date: March 10, 2011Applicant: HEMOSHEAR, LLCInventors: Brett R. Blackman, Brian R. Wamhoff
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Patent number: 7811782Abstract: Methods and devices for applying hemodynamic patterns to human/animal cells in culture are described. Hemodynamic flow patterns are measured directly from the human circulation and translated to a motor that controls the rotation of a cone. The cone is submerged in fluid (i.e., cell culture media) and brought into close proximity to the cells. Rotation of the cone creates time-varying shear stresses. This model closely mimics the physiological hemodynamic forces imparted on endothelial cells in vivo. A TRANSWELL coculture dish (i.e., a coculture dish comprising an artificial porous membrane) may be incorporated, permitting two, three, or more different cell types to be physically separated within the culture dish environment. In-flow and out-flow tubing may be used to supply media, drugs, etc. separately and independently to both the inner and outer chambers. The physical separation of the cell types permits each cell type to be separately isolated for analysis.Type: GrantFiled: January 10, 2008Date of Patent: October 12, 2010Assignee: Hemoshear, LLCInventors: Brett R. Blackman, Brian R. Wamhoff