Patents Assigned to Henan Julong Biological Engineering Co., Ltd
  • Patent number: 11453898
    Abstract: The present invention belongs to the bioengineering field, and relates to a method for fermentation production of L-theanine by using an Escherichia coli genetically engineered bacterium. The engineered bacterium is obtained by serving a strain as an original strain, wherein the strain is obtained after performing a single copy of T7RNAP, a dual copy of gmas, xylR knockout, and sucCD knockout on an Escherichia coli W3110 genome, and by integrating genes xfp, pta, acs, gltA, and ppc, and knocking out ackA on the genome. The present invention has a high yield, and stable production performance; after 20-25 h, L-theanine has a titer of 75-80 g/L, and the yield is up to 52-55%. The fermentation broth is purified by membrane separation in combination with a cation-anion resin series technique. Moreover, the one-step crystallization yield is 72.3% and the L-theanine final product has a purity of 99%.
    Type: Grant
    Filed: December 31, 2021
    Date of Patent: September 27, 2022
    Assignees: Henan Julong Biological Engineering Co., Ltd, Tianjin University of Science and Technology
    Inventors: Xiaoguang Fan, Xiaodong Liu, Jing Li, Ning Chen, Bochao Liu, Shuai Liu, Chaochao Sun, Yongchao Liu, Jiajia Teng, Mengtao Zhang, Yuanqing Ji, Yuhang Zhou, Qingyang Xu