Abstract: The invention refers to a new human liver cell line which may be used for toxicological, physiological and, in particular, gene therapeutic examinations. Fields of application are molecular biology, medicine and pharmaceutical industry. The new human liver cell line is marked positive owing to the parameters albumin and alpha-1-antitrypsin (ATT) and negative owing to alpha-fetoprotein (AFP). It has been deposited in the Deutsche Sammlung von Mikroorganismen (DSM ACC2302).