Abstract: Novel phytases derived from ruminal microorganisms are provided. The phytases are capable of catalyzing the release of inorganic phosphorus from phytic acid. Preferred sources of phytases include Selenomonas, Prevotella, Treponema and Megasphaera. A purified and isolated DNA encoding a phytase of Selenomonas ruminantium JY35 (ATCC 55785) is provided. Recombinant expression vectors containing DNA's encoding the novel phytases and host cells transformed with DNA's encoding the novel phytases are also provided. The novel phytases are useful in a wide range of applications involving the dephosphorylation of phytate, including, among other things, use in animal feed supplements.

Abstract: Novel phytases derived from ruminal microorganisms are provided. The phytases are capable of catalyzing the release of inorganic phosphorus from phytic acid. Preferred sources of phytases include Selenomonas, Prevotella, Treponema and Megasphaera. A purified and isolated DNA encoding a phytase of Selenomonas ruminantium JY35 (ATCC 55785) is provided. Recombinant expression vectors containing DNA's encoding the novel phytases and host cells transformed with DNA's encoding the novel phytases are also provided. The novel phytases are useful in a wide range of applications involving the dephosphorylation of phytate, including, among other things, use in animal feed supplements.

Abstract: An animal carcass pasteurization apparatus and process is provided. Carcasses are suspended from a movable gambrel and are conveyed through a housing containing a heated water distribution system. Preferably, the gambrel extends through the top of the housing in a scaled manner, excluding contaminants from outside of the housing. The distribution system comprises a plurality of distribution elements and means for supplying heated water thereto. The distribution elements preferably comprise overhead and lower arrays of nozzles. Each nozzle dispenses and directs a discrete and substantially continuous sheet of heated water to contact the carcass. The overhead array directs sheets of water downwardly onto the carcass, forming a layer of heated water which flows over the exterior contours of the carcass, for pasteurization of the upper and lateral surfaces of the carcass. The lower array directs heated water upwardly to contact and pasteurize the underside of the carcass.