Abstract: Described herein is a novel method for generation of recombinant poxviruses using an E3 and K3 double deletion mutant virus as the parental virus for generation of recombinant viruses. Following allowing for crossing over between the parental virus and an insertion cassette including an orthopox K3 peptide and the gene of interest, recombinant viruses are selected by infecting a host cell line permissive for the orthopox K3 peptide but not for the E3 and K3 double mutant parental virus. It is also demonstrated that a specific small molecule inhibitor of NEDD8 activating enzyme, MLN4924, can completely block poxvirus K3 family protein mediated PKR degradation and virus replication.

Abstract: Instead of generating immune responses to several HIV proteins and risk over activating more CD4+ T cells (easy targets for HIV-1 infection) as current candidate vaccines try to do, a lower magnitude, narrowly focused, well maintained virus specific CD8+ T cell response to multiple subtypes should destroy and eliminate a few founder viruses without inducing inflammatory responses that may activate more CD4+ T cells and provide more targets for HIV-1 virus infection. Specifically, described herein is a method that focuses the immune response to the 12 protease cleavage sites.

Abstract: Instead of generating immune responses to several HIV proteins and risk over activating more CD4+ T cells (easy targets for HIV-1 infection) as current candidate vaccines try to do, a lower magnitude, narrowly focused, well maintained virus specific CD8+ T cell response to multiple subtypes should destroy and eliminate a few founder viruses without inducing inflammatory responses that may activate more CD4+ T cells and provide more targets for HIV-1 virus infection. Specifically, described herein is a method that focuses the immune response to the 12 protease cleavage sites.

Abstract: Instead of generating immune responses to several HIV proteins and risk over activating more CD4+ T cells (easy targets for HIV-1 infection) as current candidate vaccines try to do, a lower magnitude, narrowly focused, well maintained virus specific CD8+ T cell response to multiple subtypes should destroy and eliminate a few founder viruses without inducing inflammatory responses that may activate more CD4+ T cells and provide more targets for HIV-1 virus infection. Specifically, described herein is a method that focuses the immune response to the 12 protease cleavage sites.

Abstract: Described herein are a number of Ebola monoclonal antibodies.

Abstract: Two universally conserved sequences from influenza type A neuraminidases were identified by large scale sequence analysis then chemically modified and conjugated to carrier proteins to generate mono-specific and monoclonal antibodies. The two antibodies, one targeting the N-terminus of the type A neuraminidase and the other sequence close to enzymatic active site, were capable of binding to all 9 subtypes of neuraminidase while demonstrating remarkable specificity against the viral neuraminidase sequences since no cross-reactivity against allantoic proteins was observed. Quantitative analyses of NA using slot blot suggest that the antibodies can be used for NA antigen quantitation in vaccines. These represent the first time the antibody-based immunoassay can be used for NA quantitative determination.

Abstract: Derivatives of dillapiol, sesamol and related monolignans having the following general formula: These compounds have synergistic properties, inhibit cytochrome P450 enzymes such as human CYP3A4, and can be used as pesticide synergists or pharmaco-enhancers. Accordingly, methods for increasing the efficacy and/or bioavailability of a pharmaceutically active agent and for increasing the potency of a pesticide are described, as are synergistic pesticidal and pharmaceutical compositions.

Abstract: Described herein are a number of Ebola monoclonal antibodies.

Abstract: The characterization and isolation of F20G75, F20G76 and F20G77, anti-PA monoclonal antibodies which also have neutralizing activities is described. The monoclonal antibodies may be used as a pharmaceutical composition for treating individuals suspected of or at risk of or having a Bacillus anthracis infection. The monoclonal antibodies bind to a specific region comprising amino acids 311-316 of PA, ASFFDI or a larger fragment comprising amino acids 301-330 of PA, SEVHGNAEVHASFFDIGSSVSAGFSNSNSS. Vaccines comprising these peptides may be used to immunize individuals against Bacillus anthracis infection.

Abstract: Adjuvant compositions comprising specific 5mer polypeptides in combination with antigen delivery systems and/or immunostimulatory molecules, such as immunostimulatory nucleic acid sequences, for enhancing the immune response of a coadministered antigen, are described.

Abstract: Described herein are a number of Ebola monoclonal antibodies.

Abstract: Described herein is the use of ultrasound pulses at different frequencies to track the dispersion properties of intracranial tissues which may have been altered due to traumatic or other neurological brain injury. Dispersive ultrasound does not provide imaging, but it can provide data of significant diagnostic value by using decision support systems that can be trained as a medical diagnostic system for traumatic brain injuries applications to detect specific patterns of dispersion that are associated with specific intracranial injuries.

Abstract: A modified CAG promoter which is capable of driving high levels of expression of sequences of interest inserted downstream therefrom is herein described.

Abstract: A system and method for software policy management are provided. A compiled policy-enabled software application includes a policy enforcement point to export an information element to a policy manager. The policy manager includes a policy interpreter having a policy decision point to perform policy decision making based on the received information element from the policy enforcement point. Through a plurality of policy decision points, the policy manager can execute a wide range of policies for different compiled software applications. Policies can be modified centrally in the policy interpreter and changes can affect either one or more of the policy-enabled software applications. A policy manager browser can create and manage the policy decision making performed by the policy interpreter.

Abstract: Derivatives of dillapiol, sesamol and related monolignans having the following general formula: These compounds have synergistic properties, inhibit cytochrome P450 enzymes such as human CYP3A4, and can be used as pesticide synergists or pharmaco-enhancers. Accordingly, methods for increasing the efficacy and/or bioavailability of a pharmaceutically active agent and for increasing the potency of a pesticide are described, as are synergistic pesticidal and pharmaceutical compositions.

Abstract: A method and system are provided for implementing a configurable browser-based form application. A configurable application toolset is provided, having a plurality of reusable browser-based form application components, or shells, that are configured in response to a received set of configuration parameters. Based on the configuration parameters, the toolset can be used to create any number of browser-based form applications. The application includes a browser-based form, a database, and rules governing the collection and storage of gathered and stored data. All of the configuration parameters are stored as data, so that no recompiling or modification of compiled software code in the shells is required when changes are needed, or an entirely new browser-based form application is desired. This reduces cost and time in software development.

Abstract: A method of aggregating and disseminating time sensitive information. A data source is searched to identify recently-posted information items matching predetermined selection criteria. A respective relevance score is calculated for each identified information item. A respective urgency rating is determined for each identified information item. Each information item is triaged using the urgency rating, and disseminated to at least one client based on the triage result.

Abstract: Relatively accurate gravimetric analysis of airborne particulate matter in a sample is achieved by making gravimetric measurements of the sample on a microbalance in a closed chamber, continuously electronically monitoring air pressure, humidity and temperature in the chamber, continuously controlling humidity and temperature in the chamber, and combining the gravimetric measurement with measurements of air pressure, humidity and temperature in the chamber to make a buoyancy corrected determination of the mass of the particulate matter.

Abstract: In a method for reproducible dissolution of a pharmaceutical product in a dissolution medium contained within a vessel, a flow regime characterized by high turbidity and low bulk movement of dissolution medium is induced within the vessel. Simultaneously, solid particles of the pharmaceutical product on a bottom portion of the vessel are mechanically dispersed. Induction of the flow regime and mechanical dispersion of solid particles may be accomplished by a brush body adapted to sweep a bottom portion of the vessel. The brush body is repeatably biased into contact with the bottom portion of the vessel, and caused to rotate in a controlled manner.

Abstract: The invention disclosed relates to a method and apparatus for solid phase microextraction of target analytes from solid or fluid samples. The apparatus comprises gas tight enclosure means, means for introducing a sample including target analytes into the enclosure means, and means located within the enclosure means for extracting the target analytes from the sample, wherein the extraction means either samples a head space near the sample or samples the sample directly.