Abstract: The invention relates to a formulation for total and differential counting of leukocytes for use in clinical analyses, in particular hemograms, to promote instantaneous differential staining of leukocytes in liquid medium, allowing greater practicality and agility in the total and differential counting of leukocytes. The practicality and agility are related to the use of a single dye for two parameters, eliminating the need for a smear. It results in a satisfactory effect for staining cells in suspension, staining the nucleus and cytoplasm of leukocytes in different shades, allowing their visualization under an optical microscope or in Point-of-Care image processing devices. It uses easily accessible raw materials, including cresyl acetate violet, ethyl alcohol, sodium and potassium chlorides, sodium hydroxide, acetic acid, triton X and deionized water, presenting a simple manufacturing process and low cost.
Type:
Application
Filed:
December 21, 2022
Publication date:
June 29, 2023
Applicant:
Hi Technologies Ltda.
Inventors:
Marcus Vinicius MAZEGA FIGUEREDO, Sergio Renato ROGAL JÚNIOR, Aléxía THAMARA GASPARIN, Claudiane ISABEL FRANCO ARAUJO, Erika BERGAMO SANTIAGO, Luis Felipe HARTMANN, Adriano Artur TIMM, Ivan LUCAS REIS SILVA, Flavia ZHU TENG, João Victor PREDEBON
Abstract: The invention relates to a formulation for counting and morphological-based inference of erythrocytes and platelets to for use in clinical analyses, in particular hemograms, with the purpose of counting both erythrocytes and platelets in a liquid medium, and performing qualitative inferences of erythrocyte and platelet morphology, through image processing in Point-of-Care devices or through an optical microscope. This formulation preserves the natural morphological characteristics of erythrocytes and platelets, and uses methylparaben, propylparaben, sodium chloride, sodium bicarbonate, bovine serum albumin (BSA), sodium citrate, potassium citrate, K2EDTA and deionized water, which is advantageous in terms of operational simplicity and low cost.
Type:
Application
Filed:
December 21, 2022
Publication date:
June 29, 2023
Applicant:
Hi Technologies Ltda.
Inventors:
Marcus Vinicius MAZEGA FIGUEREDO, Sergio Renato Rogal Júnior, Aléxia Thamara Gasparin, Claudiane Isabel Franco Araujo, Erika Bergamo Santiago, Luis Felipe Hartmann, Adriano Artur Timm, Ivan Lucas Reis Silva, Flavia Zhu Teng, João Victor Predebon
Abstract: The invention refers to a method of detecting nucleic acid sequences in biological samples from medical, agricultural and biotechnological sources, and a corresponding device, that can be used in health care, in particular in laboratorial diagnosis, to detect genetic sequences, with the objective of identifying viruses and diseases arising from genetic malformations, bringing novelties of using saliva samples, making extraction of RNA from the genetic material from the sample, through a small device with low complexity and innovative design, with the advantages of portable, rapid results, with on-line connectivity to a test results center, dispensing frequent visits to the doctor, hastening the start of treatment, allowing access for groups of people and eliminating the need for a highly qualified operator.
Type:
Application
Filed:
June 8, 2022
Publication date:
December 8, 2022
Applicant:
HI TECHNOLOGIES LTDA.
Inventors:
Marcus Vinicius MAZEGA FIGUEREDO, Sergio Renato ROGAL JÚNIOR, Luís Felipe HARTMANN, Adriano Artur TIMM, João Victor PREDEBON, Bruna MEDEIROS DA SILVA, Erika BERGAMO SANTIAGO, Bruna WINKERT RADDATZ, Rafael BENEDETTI