Patents Assigned to Hideaki Yamada
  • Gene encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant
    Patent number: 5789211
    Abstract: The present invention relates to a gene derived from Pseudomonas chlororaphis B23 strain which encodes a polypeptide having nitrile hydratase activity being capable of hydrating nitriles to amides. The invention also relates to a recombinant DNA containing the gene, and a transformant transformed with the recombinant DNA. The present invention further relates to a method of producing nitrile hydratase using the transformant and of amides using nitrile hydratase.
    Type: Grant
    Filed: June 5, 1995
    Date of Patent: August 4, 1998
    Assignees: Nitto Chemical Industry Co., Ltd., Teruhiko Beppu, Hideaki Yamada
    Inventors: Hideaki Yamada, Toru Nagasawa, Teruhiko Beppu, Sueharu Horinouch, Makoto Nishiyama
  • DNA fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant
    Patent number: 5731176
    Abstract: The present invention has disclosed the amino acid sequence and nucleotide sequence of the .alpha.- and .beta.-subunits of two types of nitrile hydratase derived from Rhodococcus rhodochrous J-1. The DNA fragment encoding nitrile hydratase is inserted into an expression vector and the recombinant vector is used for transformation. The transformant contains multiple copies of the gene and can produce much higher level of nitrile hydratase compared with conventionally used microorganisms.
    Type: Grant
    Filed: March 9, 1993
    Date of Patent: March 24, 1998
    Assignees: Nitto Chemical Industry Co., Ltd., Teruhiko Beppu, Hideaki Yamada
    Inventors: Hideaki Yamada, Toru Nagasawa, Teruhiko Beppu, Sueharu Horinouch, Makoto Nishiyama
  • Manufacture of optically active 2-phenylpropionic acid and 2-phenylpropionamide from the nitrile using Rhodococcus equi
    Patent number: 5258305
    Abstract: The present invention provides a method of obtaining optically active 2-phenylpropionic acid and 2-phenylpropionamide such as S-(+)-2-phenylpropionic acid, R-(-)-2-phenylpropionamide, and R-(-)-2-phenylpropionic acid from S-2-phenylpropionitrile or R,S-2-phenylpropionamide using a bacterium belonging to Rhodococcus equi.Optically active 2-phenylpropionic acid and 2-phenylpropionamide are recovered in high yield and in quantities according to the method of the invention.
    Type: Grant
    Filed: March 12, 1992
    Date of Patent: November 2, 1993
    Assignees: Nitto Chemical Industry Co., Ltd., Lonza Japan, Ltd., Hideaki Yamada
    Inventors: Hideaki Yamada, Toru Nagasawa
  • Process for biological production of organic acids
    Patent number: 5135858
    Abstract: An improved biological conversion of a nitrile such as acrylonitrile or a cyanopyridine into the corresponding carboxylic acid such as acrylic acid or a nicotinic acid by the action upon the nitrile of a nitrilase enzyme, in which the improvement resides in the use as the source of the enzyme of a microorganism of Rhodococcus, such as Rh. rhodochrous J-1, FERM BP-1478, which is cultured in the presence of a lactam compound.
    Type: Grant
    Filed: February 22, 1991
    Date of Patent: August 4, 1992
    Assignees: Hideaki Yamada, Nitto Kagaku Kogyo Kabushiki Kaisha
    Inventors: Hideaki Yamada, Toru Nagasawa, Tetsuji Nakamura
  • Method of culturing a strain of Rhodococcus rhodochrous having nitrile hydratase activity
    Patent number: 5089411
    Abstract: Cells of bacteria of the species Rhodococcus rhodochrous having a high nitrile hydratase activity can be obtained in a high yield by adding to a culture medium at least one of urea and its derivatives and cobalt ion in the preparation of cells of bacteria having nitrile hydratase activity by cultivating Rhodococcus rhodochrous bacteria capable of producing nitrile hydratase.
    Type: Grant
    Filed: October 5, 1989
    Date of Patent: February 18, 1992
    Assignees: Hideaki Yamada, Nitto Kagaku Kogyo Kabushiki Kaisha
    Inventors: Hideaki Yamada, Toru Nagasawa
  • Method of cultivation of pseudomonas bacteria
    Patent number: 4880739
    Abstract: Cells of Pseudomonas bacteria having a high nitrile hydratase activity can be obtained in a high yield by adding sequentially to a culture medium at least one compound selected from the group consisting of propionitrile, isobutyronitrile, propionamide, and isobutyramide in the process of cultivation of Pseudomonas bacteria capable of producing nitrile hydratase.
    Type: Grant
    Filed: October 2, 1987
    Date of Patent: November 14, 1989
    Assignees: Hideaki Yamada, Nitto Kagaku Kogyo Kabushiki Kaisha
    Inventors: Hideaki Yamada, Kanehiko Enomoto, Ichiro Watanabe
  • Method for cultivation of pseudomonas bacteria
    Patent number: 4661456
    Abstract: Cells of Pseudomonas bacteria having a high nitrile hydratase activity can be obtained in a high yield by adding to a culture medium at least one .alpha.-amino acid, except for cysteine or cystine alone and a combination of these .alpha.-amino acids alone, in the preparation of cells of bacteria having nitrile hydratase activity by cultivating under nitrile hydratase-inducing conditions Pseudomonas bacteria capable of producing nitrile hydratase.
    Type: Grant
    Filed: January 9, 1984
    Date of Patent: April 28, 1987
    Assignees: Nitto Kagaku Kogyo Kabushiki Kaisha, Hideaki Yamada
    Inventors: Hideaki Yamada, Koitchiro Ryuno, Kanehiko Enomoto, Ichiro Watanabe
  • Method for cultivation of pseudomonas bacteria
    Patent number: 4661457
    Abstract: Cells of Pseudomonas bacteria having a high nitrile hydratase activity can be obtained in a high yield by adding to a culture medium cysteine and (or) cystine in the preparation of cells of bacteria having nitrile hydratase activity by cultivating under nitrile hydratase-inducing conditions Pseudomonas bacteria capable of producing nitrile hydratase.
    Type: Grant
    Filed: January 9, 1984
    Date of Patent: April 28, 1987
    Assignees: Nitto Kagaka Kogyo Kabushiki Kaisha, Hideaki Yamada
    Inventors: Hideaki Yamada, Kanehiko Enomoto, Ichiro Watanabe
  • Process for biological preparation of amides
    Patent number: 4637982
    Abstract: In a process for hydrating a nitrile having 2 to 4 carbon atoms by a microbiological treatment to convert the nitrile into the corresponding amide, it is possible to biologically prepare the corresponding amide effectively by utilizing a microorganism of the genus Pseudomonas which is capable of hydrating the nitrile to convert the same into the corresponding amide. In particular, it is possible to obtain acrylamides which are highly useful polymers by the very simple and energy-saving process in accordance with the present invention.
    Type: Grant
    Filed: July 5, 1983
    Date of Patent: January 20, 1987
    Assignees: Hideaki Yamada, Nitto Kagaku Kogyo Kabushiki Kaisha
    Inventors: Hideaki Yamada, Yoshiki Tani
  • Method for cultivation of Pseudomonas bacteria
    Patent number: 4555487
    Abstract: Cells of Pseudomonas bacteria having a high nitrile hydratase activity can be obtained in a high yield by adding to a culture medium at least one amide compound selected from the group consisting of acrylamide, methacrylamide, crotonamide, and n-butyramide in the preparation of cells of bacteria having nitrile hydratase activity by cultivating Pseudomonas bacteria capable of producing nitrile hydratase.
    Type: Grant
    Filed: November 15, 1983
    Date of Patent: November 26, 1985
    Assignees: Nitto Kagaku Kogyo Kabushiki Kaisha, Hideaki Yamada
    Inventors: Hideaki Yamada, Koitchiro Ryuno