Patents Assigned to Hitachi Chemical Research Center
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Patent number: 7645582Abstract: Aptamers bind to Listeria surface proteins. A method of assaying a sample for the presence of Listeria monocytogenes includes exposing the sample to an aptamer that specifically binds one of the following proteins: Listeria monocytogenes internalin A protein, Listeria monocytogenes internalin E protein, and Listeria monocytogenes 0610 protein. The presence of Listeria monocytogenes in the sample is detected when the aptamer binds the protein present in the sample. A method of treating Listeria monocytogenes infection includes administering the aptamers to the mammal at a concentration sufficient to reduce Listeria monocytogenes infection.Type: GrantFiled: April 13, 2009Date of Patent: January 12, 2010Assignees: Hitachi Chemical Co., Ltd., Hitachi Chemical Research Center Inc.Inventors: Cindy Yamamoto, Toshit Sen
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Publication number: 20090301875Abstract: An ionic polymer composite device and methods for fabricating the ionic polymer composite device are provided. The ionic polymer composite device includes two extended electrode layers, each extended electrode layer including at least one ionic polymer with a plurality of electrically conductive particles, and a dielectric layer including at least one sulfonated poly ether sulfone polymer or a derivative between the two extended electrode layers.Type: ApplicationFiled: July 3, 2009Publication date: December 10, 2009Applicants: Hitachi Chemical Co., Ltd., Hitachi Chemical Research Center, Inc.Inventors: Yongxian Wu, Bun-Ichiro Nakajima, Shinji Takeda, Iwao Fukuchi, Naoki Asano
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Publication number: 20090253133Abstract: A method is disclosed for determining whether a human having Crohn's disease is likely to respond to a therapy targeting a TNFSF member or a cytokine by measuring the level of certain mRNAs in response to a stimulus. A method of evaluating the effectiveness of a Crohn's disease therapy in a human is also disclosed. Furthermore, a method of screening compounds for use in the treatment of Crohn's disease is disclosed. A method of monitoring the disease state over time in Crohn's disease patients is also disclosed.Type: ApplicationFiled: April 5, 2007Publication date: October 8, 2009Applicants: HITACHI CHEMICAL CO., LTD, HITACHI CHEMICAL RESEARCH CENTER, INC, CEDARS-SINAI MEDICAL CENTERInventors: Masato Mitsuhashi, Stephan R. Targan
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Patent number: 7595155Abstract: The present invention comprises detection probes utilizing vesicles or soluble bodies to retain multiple mass tag molecules. The detection probes may be used to simultaneously assay a plurality of different biological samples, each comprising a plurality of analytes, by immobilizing the analytes from each of the samples on a surface incubating the surface with a set of the detection probes, each having mass tag molecules with different masses, removing the unbound detection probe, collecting the first and second mass tag molecules from the bound detection probe, and quantifying the first and second mass tag molecules collected.Type: GrantFiled: February 28, 2005Date of Patent: September 29, 2009Assignees: Hitachi Chemical Research Center, Hitachi Chemical Co., Ltd.Inventor: Taku Murakami
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Publication number: 20090215064Abstract: Heparinized human whole blood from patients with invasive breast cancer, with (multiple primary) and without (single primary) a second primary cancer, and from unaffected controls was stimulated with 0.1-10 Gy of radiation and incubated at 37° C. for 2 hours. P21 and PUMA mRNA were then quantified. The results suggest that cancer susceptibility represented by the multiple primary cases was significantly related to over-reaction of p21 mRNA, and not PUMA.Type: ApplicationFiled: February 27, 2009Publication date: August 27, 2009Applicants: HITACHI CHEMICAL CO., LTD., HITACHI CHEMICAL RESEARCH CENTER, INC., THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Masato Mitsuhashi, Hoda Anton-Culver, Argyrios Ziogas, David Peel
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Publication number: 20090203028Abstract: Aptamers bind to Listeria surface proteins. A method of assaying a sample for the presence of Listeria monocytogenes includes exposing the sample to an aptamer that specifically binds one of the following proteins: Listeria monocytogenes internalin A protein, Listeria monocytogenes internalin E protein, and Listeria monocytogenes 0610 protein. The presence of Listeria monocytogenes in the sample is detected when the aptamer binds the protein present in the sample. A method of treating Listeria monocytogenes infection includes administering the aptamers to the mammal at a concentration sufficient to reduce Listeria monocytogenes infection.Type: ApplicationFiled: April 13, 2009Publication date: August 13, 2009Applicants: HITACHI CHEMICAL RESEARCH CENTER, INC., HITACHI CHEMICAL CO., LTD.Inventors: CINDY YAMAMOTO, TOSHIT SEN
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Publication number: 20090189111Abstract: A sound attenuating material includes a matrix, and a nanofiller, the material effectively reduces the level of low frequency sound incident thereon.Type: ApplicationFiled: August 16, 2007Publication date: July 30, 2009Applicants: HITACHI CHEMICAL CO., LTD., HITACHI CHEMICAL RESEARCH CENTER, INC.Inventor: Shahram Zamani
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Publication number: 20090111128Abstract: Tumor necrosis factor (TNF) is capable of inducing apoptosis by interacting with specific TNF receptors on the surface of cancer cells. Because multiple members of TNF ligand and receptor are present within each superfamily, over 300 different ligand-receptor combinations exist. Activated blood leukocytes produce TNF as part of the immune response to cancer, as well as producing chemokines to attract other leukocytes to the site. A method is disclosed of detecting significant induction of a variety of TNF superfamily subtype and chemokine mRNAs in blood leukocytes when whole blood is exposed to heat-aggregated IgG or anti-T cell receptor antibodies as a model of immune system interactions. Substantial individual-to-individual variation is observed in TNF subtypes and chemokines induced. Since peripheral blood leukocytes are the supply of anti-cancer immune cells, the quantitation of ex vivo inducibility of appropriate TNF ligands and chemokines in blood will be useful in individualized cancer immunotherapy.Type: ApplicationFiled: June 8, 2006Publication date: April 30, 2009Applicants: HITACHI CHEMICAL RESEARCH CENTER INC., HITACHI CHEMICAL CO., LTD.Inventor: Masato Mitsuhashi
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Publication number: 20090032394Abstract: An embodiment provides an ionic polymer device comprising two extended electrode layers comprising a plurality of conductive particles, wherein the plurality of conductive particles form a concentration gradient in each of the two extended electrode layers, an ionic polymer dielectric layer between two extended electrode layers, and at least one conductive layer on outer surfaces of two extended electrode layers. Another embodiment provides an ionic polymer device comprising a polymer composite with a plurality of surface features on two opposite surfaces, and at least one conductive layer on each of said two opposite surfaces. One embodiment provides a method of making an ionic polymer device, comprising forming a partially cured polymer-metallic salt layer, reducing the metallic salt to form a plurality of metal particles, thereby forming a first extended electrode layer and a second extended electrode layer at and near opposite surfaces of the ionic polymer device.Type: ApplicationFiled: January 23, 2007Publication date: February 5, 2009Applicants: HITACHI CHEMICAL CO., LTD., HITACHI CHEMICAL RESEARCH CENTER, INC.Inventors: Yongxian Wu, Yangyang Li
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Patent number: 7374881Abstract: The present invention is related to a novel method of detecting mRNA, based on the fact that the newly synthesized mRNA is confined inside the cell nucleus. According to the novel method cells are captured on a membrane and then treated by a cell membrane permeation solution, thereby increasing permeability of cell membranes. After the cytoplasm is washed away, nuclei are dissolved with a cell dissolving solution, and then mRNA can be successfully recovered from the obtained solution.Type: GrantFiled: October 25, 2001Date of Patent: May 20, 2008Assignees: Hitachi Chemical Research Center, Inc., Hitachi Chemical Co., LtdInventor: Masato Mitsuhashi
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Patent number: 7285199Abstract: A method and apparatus for concentrating dilute biomolecules in samples using electrophoresis are described. The method involves using the charged nature of the biomolecules to localize them to a microspot containing a dialysis membrane. The method and apparatus may also be used for identifying the presence of a biomolecule in a mixture or to quantitate the amount of a specific biomolecule in a mixture. The method and apparatus may also be used to produce a high throughput method for analysis and quantitation of a sample containing biomolecules.Type: GrantFiled: October 31, 2001Date of Patent: October 23, 2007Assignees: Hitachi Chemical Research Center, Inc., Hitachi Chemical Co., Inc.Inventors: Masato Mitsuhashi, Taku Murakami, Tsuruki Tamura, Masafumi Yohda, Mina Okochi
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Patent number: 7258976Abstract: The entire process of reverse transcription-polymerase chain reaction (RT-PCR) is simplified by using oligonucleotide-immobilized microplates made of, e.g., polypropylene, to which oligonucleotides are securely immobilized and which can be subjected to thermal cycles of PCR. RT-PCR is preferably conducted in solid-phase. Capturing of mRNA and RT-PCR can be conducted in the same plates. The cDNA synthesized from the mRNA captured on the microplates can be used more than once. Further, in combination with the microplates, a filter plate is used for the preparation of cell lysates wherein target cells are placed on the filter plate, and a lysis buffer is passed through the cell layer on the filter to transfer cell lysate directly to the microplate via well-to-well communication.Type: GrantFiled: March 31, 2003Date of Patent: August 21, 2007Assignees: Hitachi Chemical Co. Ltd., Hitachi Chemical Research Center, Inc.Inventor: Masato Mitsuhashi
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Patent number: 7214781Abstract: A method for the diagnosis and identification of new or residual lung cancer is disclosed which uses newly identified markers for lung cancer including syndecan 1, collagen 1 alpha 2, and two novel proteins, 7013 and 7018. The method involves identification of the lung cancer markers is blood from a patient. It is envisioned that at least one marker may be used or any mixture of the four. The method may also include the identification of cytokeratin-19.Type: GrantFiled: June 21, 2001Date of Patent: May 8, 2007Assignees: Hitachi Chemical Research Center, Inc., Hitachi Chemical Co., Ltd.Inventors: Masato Mitsuhashi, Hiroko Matsunaga, Hideki Kambara, Masafumi Kawamura
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Patent number: 6844158Abstract: The entire process of reverse transcription-polymerase chain reaction (RT-PCR) is simplified by using oligonucleotide-immobilized microplates made of, e.g., polypropylene, to which oligonucleotides are securely immobilized and which can be subjected to thermal cycles of PCR. RT-PCR is preferably conducted in solid-phase. Capturing of mRNA and RT-PCR can be conducted in the same plates. The cDNA synthesized from the mRNA captured on the microplates can be used more than once. Further, in combination with the microplates, a filter plate is used for the preparation of cell lysates wherein target cells are placed on the filter plate, and a lysis buffer is passed through the cell layer on the filter to transfer cell lysate directly to the microplate via well-to-well communication.Type: GrantFiled: December 22, 1998Date of Patent: January 18, 2005Assignees: Hitachi Chemical Co., Ltd., Hitachi Chemical Research Center, Inc.Inventor: Masato Mitsuhashi
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Patent number: 6755384Abstract: A flexible platform or plate holder for use with liquid handling robots. More specifically a flexible platform for use with a variety of plate holders which allows for complete removal of a sample from a well without the need for liquid sensors.Type: GrantFiled: April 18, 2002Date of Patent: June 29, 2004Assignees: Hitachi Chemical Co., Ltd., Hitachi Chemical Research Center, Inc.Inventor: Elliott Gorfain
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Patent number: 6638428Abstract: Formation of bubbles is prevented during filtration operation by placing an oil on top of a solution-to-be filtered, where the oil is not admixable with the solution and has a lower specific gravity than the solution. The oil seals the solution from air so that no bubbles are formed. The oil does not pass through the filter even after all collectable filtrate of the solution has passed therethrough.Type: GrantFiled: October 31, 2001Date of Patent: October 28, 2003Assignees: Hitachi Chemical Research Center, Inc., Hitachi Chemical Co., LTDInventor: Mieko Ogura
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Patent number: 6533255Abstract: An apparatus for temperature control of biological/chemical samples employing liquid metal is described. A gallium-indium alloy may be used to provide excellent temperature control. Methods of using liquid metal to provide temperature control for biological/chemical samples are also described. A preferred use for the described liquid metal-heating apparatus is to provide precise temperature control for polymerase chain reaction (PCR).Type: GrantFiled: October 23, 2001Date of Patent: March 18, 2003Assignees: Hitachi Chemical Research Center, Inc., The Regents of the University of CaliforniaInventors: Masato Mitsuhashi, Charles Y. Chu
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Patent number: 6300058Abstract: The present invention provides a method for detecting and quantifying mRNA in a sample. The mRNA that can be detected has a unique sequence. The method includes immobilizing a first polynucleotide to an insoluble support. The first polynucleotide has a first sequence that hybridizes to the unique sequence on the mRNA. After immobilization of the first polynucleotide, the sample is applied to the insoluble support under conditions that allow the unique sequence on the mRNA to hybridize with the first polynucleotide. Thereafter, a second polynucleotide is applied to the insoluble support. This second polynucleotide has a second sequence thereon that hybridizes to a portion of the mRNA other than the unique sequence. The application of the second polynucleotide is performed under conditions that allow the second polynucleotide to hybridize with mRNA immobilized on said support, if present.Type: GrantFiled: November 12, 1992Date of Patent: October 9, 2001Assignees: Hitachi Chemical Research Center, Inc., Hitachi Chemical Company, Ltd.Inventors: Tatsuo Akitaya, Masato Mitsuhashi, Allan Cooper
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Patent number: 6251247Abstract: Degradation of RNA present in a sample is detected by using, as an indicator, rRNA included in the RNA. The steps include: (a) introducing the sample onto a microchannel, at an introducing point, filled with an electrophoresis separation gel; (b) conducting electrophoresis of rRNA fragments present in the sample to force rRNA fragments to migrate through the electrophoresis separation gel; (c) detecting rRNA fragments passing through a detection point located downstream of the introducing point, to obtain detection patterns; and (d) determining degradation of RNA present in the sample based on the detection patterns of rRNA. A sample containing degraded mRNA can also be screened by detecting rRNA degradation in the above microchannel electrophoresis.Type: GrantFiled: March 30, 1999Date of Patent: June 26, 2001Assignees: Hitachi Chemical Co., Ltd., Hitachi Chemical Research Center, Inc.Inventors: Masato Mitsuhashi, Mieko Ogura, Kenji Watanabe, Yoichi Agata
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Patent number: 6054437Abstract: A method for inducing cell death in neoplastic cells, includes the step of administering a compound of formula I or a pharmaceutically acceptable salt thereof, as a primary chemotherapeutic agent or substantially contemporaneously with chemotherapy, to a patient having multidrug-resistant neoplastic cells, in an amount sufficient to induce cell death in the neoplastic cells: ##STR1## where R.sup.1 is hydroxyl, C1-4 alkoxyl, C1-4 alkylcarboxyloxymethoxyl, phenyl C1-2 alkylamino group, 2,5-pyrrolidinedione-1-alkoxyl (C1-4), or ##STR2## wherein X.sup.1 is a chemical bond or C1-2 alkylene, X.sup.2 is hydrogen or carboxyl forming a 5-membered ring with X.sup.1 when X.sup.1 is methylene, X.sup.3 is hydrogen or C1-2 alkyl, X.sup.4 is hydrogen or C1-2 alkyl, or X.sup.3 and X.sup.4 together form a 5-membered ring, in which at least one of X.sup.2, X.sup.3, and X.sup.4 is hydrogen,R.sup.2 is C3-4 alkyl, R.sup.3 is C1-4 alkyl, ##STR3## in which n is an integer of 0 to 3.Type: GrantFiled: December 10, 1997Date of Patent: April 25, 2000Assignees: Hitachi Chemical Research Center, Inc., Hitachi Chemical Co., Ltd., Nippon Chemiphar Co., Ltd.Inventors: Gabriel F. Eilon, John W. Jacobs