Patents Assigned to Hooper Trading Co.
  • Patent number: 4517293
    Abstract: An improved in vitro cell culture process for producing in high yield and purity a serum-free and mitogen-free interleukin-2-containing conditioned supernatant. The incubation steps during stimulation and conditioning of the interleukin-2 producing cells is carried out with a rapidly rotating roller bottle culture system. Yields of interleukin-2 are on the order of 10-fold and higher than obtained by similar cultivations carried out in flat dishes or tubes or in a roller bottle culture system rotated at conventional speeds. Improvement in yields in both the static and roller culture systems are also achieved by incubating the IL-2 producing cells under high oxygen concentrations, for example atmospheres of at least 70% O.sub.2 and at least 5% CO.sub.2. In addition to using peripheral mononuclear blood cells (PBL) as the source of leukocytes containing the IL-2 producer cells, it is also possible to use buffy coat cells which are readily available as a waste by-product from blood banks.
    Type: Grant
    Filed: February 3, 1984
    Date of Patent: May 14, 1985
    Assignee: Hooper Trading Co. N.V.
    Inventors: Fabricius, Roland Stahn
  • Patent number: 4464355
    Abstract: A serum-free and mitogen-free T-cell growth factor which is effective in stimulating natural killer cell function in tumor patients is provided. A method of producing the growth factor from human, bovine or porcine spleen or blood is described.
    Type: Grant
    Filed: September 3, 1982
    Date of Patent: August 7, 1984
    Assignee: Hooper Trading Co.
    Inventors: Hans-Ake Fabricius, Roland Stahn