Abstract: The present invention relates to a method for identification of common patient-spanning tumor-specific T cell receptors (TCRs) and their corresponding antigens. The invention also relates to these TCR sequences, a nucleic acid encoding the TCR, and a T cell comprising the TCR and/or the encoding nucleic acid.

Abstract: Described herein are methods for providing a tumour-specific T cell preparation of use for treating cancer. The method encompasses, determining which T cell receptors are most likely to be reactive to a cancer in a patient, then preparing a T cell population expressing these specific T cell receptor genes from expression constructs transferred into a preparation of T cells, and administering these engineered T cells into the patient.

Abstract: Described herein is a method for amplifying a target nucleic acid sequence ta-tC-tV-tC?-tn? comprising a first amplification using a first primer pair with sequence ma-K-pC and ma-K?-pC?, and a subsequent second amplification using a second primer pair with sequence aL-aP-aK and aL?-aP?-aK?, wherein pC is the same sequence as sequence element tC. pC and pC? are 8 to 40 nucleotides in length, K comprises a 3?-terminal sequence k1-k2-s, s is a mismatch sequences preventing PCR bias, ak is the same sequence as sequence element k1, aP-aK hybridize to a contiguous sequence on sequence element ma-K, k1 is 2 to 9 nucleotides in length, aL and aL? can be any sequence, and tV is a variable region within the target nucleic acid sequence. Also described are collections of primer sets for use in the method of the invention.

Abstract: The present invention relates to a method for providing a tumour specific T cell preparation, comprising the steps of: a. selecting tumour-specific T cell clones by: —providing a tumour sample obtained from a patient; —isolating a nucleic acid preparation from the tumour sample in a nucleic acid isolation step; —obtaining a plurality of T cell receptor nucleic acid sequences from the nucleic acid preparation or a plurality of T cell receptor amino acid sequences encoded by the plurality of T cell receptor nucleic acid sequences; —selecting a tumour-specific T cell receptor nucleic acid sequence from the plurality of T cell receptor nucleic acid sequences or a tumour-specific T cell receptor amino acid sequence from the plurality of T cell receptor amino acid sequences in a sequence selection step; b.

Abstract: The invention relates to a method for amplifying a target nucleic acid sequence tn-tC-tV-tC?-tn? comprising a first amplification using a first primer pair with sequence ma-K-pC and ma-K?-pC?, and a subsequent second amplification using a second primer pair with sequence aL-aP-aK and aL?-aP?-aK?, wherein pC is the same sequence as sequence element tC. pC and pC? are 8 to 40 nucleotides in length, K comprises a 3?-terminal sequence k1-k2-s, s is a mismatch sequences preventing PCR bias, ak is the same sequence as sequence element k1, aP-aK hybridize to a contiguous sequence on sequence element ma-K, k1 is 2 to 9 nucleotides in length, aL and aL? can be any sequence, and tV is a variable region within said target nucleic acid sequence. The invention further relates to collections of primer sets for use in the method of the invention.