Abstract: A PAM-less CRISPR/Cas gene editing system is provided. Specifically, the PAM-less CRISPR/Cas gene editing system offers Gs12-10, a PAM-less endonuclease identified through functional metagenomic mining approach, which can cleave target DNA at nearly any position in the genome. This technology enables a nucleic acid visualization detection and genome editing platform mediated by the CRISPR/Gs12-10 system, holding broad application potential in genome-modification and nucleic acid detection fields.

Abstract: Provided are a protein containing an amino acid sequence capable of binding to a peptide fragment of a substrate GPRP, a fibrinogen-like protein 1 (FGL1) containing the amino acid sequence, and a fibrinogen domain (FD) of the FGL1, wherein a single FD protein also has a function the same as or similar to that of the FGL1. The FGL1 and FD, which have different action mechanisms from existing drugs, can inhibit fibrin assembly in the process of thrombogenesis by means of competing for fibrin substrate binding pockets. The FGL1 and FD can have a stronger affinity to the substrate by means of mutation, thereby effectively enhancing a usage effect of drugs for treating thrombus.

Abstract: A nucleic acid endonuclease with high activity and high heat resistance and a gene editing system mediated by the nucleic acid endonuclease are provided. Specifically, the present invention provides a nucleic acid endonuclease Gs12-7 with a wide temperature range identified by metagenomics combined with experiments, which has the advantages of high protein temperature tolerance, recognition of PAM sequences containing BTYV, and thus has a larger gene editing space and high activity and specificity in cleaving target DNA in the genome. The present invention establishes a nucleic acid visualization detection and genome targeted editing technology mediated by the CRISPR/Gs12-7 system, which has broad application prospects in the field of genome targeted modification and nucleic acid detection.

Abstract: The invention discloses a cattle shed, comprising a cattle bedding, a feeding channel, a fixed roof, a movable roof, a feeding trough, a peripheral rail fence, and a rail fence gate, wherein the cattle bedding is provided with no dividers and needs no hardening and daily manure cleaning, which is merged with playground and enclosed. The feeding channel is hardened and higher than the cattle bedding, the fixed roof and the movable roof cover the cattle bedding and the feeding trough, the peripheral rail fence and the rail fence gate surround the cattle bedding and the feeding trough, and the fixed roof and the movable roof are arranged in a slope mode, and the movable roof can be opened to allow sunlight to enter and exchange air to form convection. The cattle shed of the present invention can reduce the construction cost, open the movable roof, effectively utilize the sunlight and the air convection, ensure a complete rain-sewage separation, and realize zero discharge of manure and sewage.

Abstract: Provided is a pig genome-wide specific sgRNA library, a preparation method therefor, and an application thereof. The sgRNA is targeted at a pig genome-wide protein-coding gene, lincRNA and/or miRNA. Specifically, an sgRNA construct has the following structure: AL-N20-AR, wherein AL is the left homology arm sequence located at the upstream of the coding sequence of a pig specific SgRNA, N20 is the coding sequence of the pig specific SgRNA, and AR is a right homology arm sequence located at the downstream of the coding sequence of the pig specific sgRNA. The sgRNA library can be used for screening functional genes of a pig or for preparing a kit.

Abstract: The field is related to plant breeding and methods of identifying and selecting plants with resistance to southern corn rust. Provided are methods to identify novel genes that encode proteins providing plant resistance to southern corn rust and uses thereof. These disease resistant genes are useful in the production of resistant plants through breeding, transgenic modification, or genome editing.

Abstract: Bifidobacterium animalis and application of a compound bacterium preparation prepared from the Bifidobacterium animalis in the preparation of a medicine for treating or preventing avian influenza virus infection. The invention discovers that Bifidobacterium animalis ATCC Accession No. 25527 can be used for treating animals infected by H7N9, and after the Bifidobacterium animalis ATCC Accession No. 25527 is compounded with Bifidobacterium pseudolongum ATCC Accession No. 25526, the effect is better. The compound preparation can regulate the body immune response, remarkably improve the weight loss and lung tissue injury caused by H7N9 influenza virus infection, and obviously improve the survival rate of mice infected by H7N9 influenza virus. The compound probiotic preparation provided by the invention has a remarkable effect of resisting H7N9 influenza virus infection, and can be effectively used for preventing and treating avian influenza virus infection.

Abstract: Application of valine in the preparation of a medicine for treating or preventing avian influenza virus infection. In a lethal mouse influenza infection model, the use of valine to treat infected mice can effectively improve the weight loss of mice, increase the survival rate of mice, and can effectively reduce the virus titer in the supernatant of the lung tissue of the infected mice.

Abstract: Application of a fragment of an isolated nucleotide sequence in the construction of zebrafish without intermuscular bones. The nucleotide sequence is shown in SEQ ID NO:1. Gene mutation is performed by taking SEQ ID NO:1 as a target gene; the mutant F0 embryos are selected and cultured to adult fish; F0 mutant is hybridized with wild type zebrafish to generate an F1 embryos; sense mutant heterozygotes F1 is screened out and cultured to adult fish; and then F1 heterozygote self-crosses to generate F2 generation of three gene types, including homozygote, heterozygote, and wild type. Zebrafish without intermuscular bones is obtained by using a gene mutation method, which provided a basis for subsequent research on a molecular formation mechanism of fish intermuscular bones and the cultivation of economic fishes without intermuscular bone and possessed a basic research value and an application value in other economic aquaculture fish species.

Abstract: The present disclosure is related to plant breeding and methods of identifying and selecting plants with disease resistance. Provided are methods to identify novel genes that encode proteins providing plant disease resistance and uses thereof. These disease resistant genes are useful in the production of resistant plants through breeding, transgenic modification, or genome editing.

Abstract: The present invention provides a method for increasing wheat yield and preventing wheat diseases and use thereof, and relates to the technical field of crop cultivation. In the method, wheat seeds are primed by a fermentation liquid of S. sclerotiorum, and the concentration of the fermentation liquid is 1.4×105 cfu/mL or more. According to the present invention, the wheat seeds are primed by using S. sclerotiorum, and S. sclerotiorum can endogenously grow in wheat plants to induce expression of genes related to disease resistance and development of wheat, thereby achieving the objective of controlling diseases such as wheat Fusarium head blight and improving wheat yield.

Abstract: A preparation method of a resin-based iron oxide-containing composite phosphate removal adsorbent is provided. An alkaline anion resin is taken as a base, a potassium ferrate is used as an iron source, and a characteristics of ferrate ions easily adsorbed on a surface of the anion resin are utilized to prepare resin-based iron oxide-containing composite phosphate removal adsorbent by one-step in-situ hydrolysis precipitation, compared with the related art, a preparation process of the disclosure is relatively simpler, a time period is shorter, and a production cost is lower. It has a strong ability to eliminate interference from other anions in the waste effluents, and it has a strong adsorption capacity, fast adsorption speed and large adsorption capacity for the phosphate. Moreover, it has the advantages of strong regeneration ability and multiple repeated use times.

Abstract: The invention provides an AMH-INH-GNIH tri-expression gene vaccine capable of improving fecundity of animals and a preparation method of engineering strain thereof. The engineering strain was deposited in China Center for Type Culture Collection on Aug. 15, 2018, with deposit No.: CCTCC NO:M 2018544. When the engineering strain is used for direct immunization of animals or immunization of animals after being mixed with DNA vaccine adjuvant, the fecundity of the animals can be effectively improved. The tri-expression gene is a tri-expression non-resistant DNA plasmid of Mullerian duct resisting hormone, inhibin and gonadotropin release restraining hormone, which can be used for direct immunization of the animals through mucosa immunization to generate antibodies in the manner of being sprayed to noses, orally administered, blended into feeds and the like.

Abstract: A tree eggplant and a cultivation method, a rapid propagation method and an application thereof are provided by the present disclosure. In the present disclosure, the Solanum wrightii is specifically selected as the female parent, and the Solanum torvum is used as the male parent, and the tree eggplant variety obtained by hybridization has fast growth, developed trunk, strong growth potential, and is perennial, and is strong in photosynthesis, strong in nutrient and water absorption capacity, high in affinity with the scion, fast in healing at the scion interface of the rootstock and good in healing; and has good resistance to soil-borne diseases such as fusarium wilt, verticillium wilt, bacterial wilt and root knot nematode; and the rapid propagation method of the tree eggplant has strong operability and high efficiency, and is easy to popularize in practical application.

Abstract: A method for controlling the building of architecture of gramineous crops and application thereof. The gene CYC U4;1 (Os10g41430) has a specific expression in a rice pulvinus (which is believed to have a specific expression in other gramineous crops), with a CDS sequence as shown in SEQ ID NO: 2, and an encoded amino acid sequence as shown in SEQ ID NO: 3. Also a promoter sequence of the gene, with the sequence as shown in SEQ ID NO: 1. Transgenic lines obtained by cloning a promoter and full-length CDS of CYC U4;1 to pCAMBIA1301 and transferring this into rice Nipponbare are all characterized by having smaller leaf-stem angles than those of wild rice, accordingly, the expression level of the gene CYC U4;1 can be increased or decreased with genetic engineering technology to control the plant architecture development, thereby improving the plant architecture and increasing the density of germplasm.

Abstract: The invention discloses a cattle shed, comprising a cattle bedding, a feeding channel, a fixed roof, a movable roof, a feeding trough, a peripheral rail fence, and a rail fence gate, wherein the cattle bedding is provided with no dividers and needs no hardening and daily manure cleaning, which is merged with playground and enclosed. The feeding channel is hardened and higher than the cattle bedding, the fixed roof and the movable roof cover the cattle bedding and the feeding trough, the peripheral rail fence and the rail fence gate surround the cattle bedding and the feeding trough, and the fixed roof and the movable roof are arranged in a slope mode, and the movable roof can be opened to allow sunlight to enter and exchange air to form convection. The cattle shed of the present invention can reduce the construction cost, open the movable roof, effectively utilize the sunlight and the air convection, ensure a complete rain-sewage separation, and realize zero discharge of manure and sewage.

Abstract: Various liquid and semisolid lubricant compositions are provided, in particular lubricant compositions containing oil from the seeds of the Brassicaceae Orychophragmus violaceus, preferably those that have been esterified with one or more fatty acids such as palmitoleic acid, oleic acid, linoleic acid, lauric acid, palmitic acid, stearic acid, or a combination thereof. In various aspects, lubricant compositions are provided that include a petroleum or a synthetic base oil and about 40% or less by weight of a liquid lubricant composition containing oil from the seeds of the Brassicaceae Orychophragmus violaceus, preferably those that have been esterified with one or more fatty acids. In various aspects, semisolid lubricant composition are provided containing an emulsion of (i) a thickener and (ii) an oil from the seeds of the Brassicaceae Orychophragmus violaceus, preferably those that have been esterified with one or more fatty acids.

Abstract: 1,4-dicaffeoylquinic acid is used as a xanthine oxidase inhibitor and in preparation of a medicament for treating gout. The present invention is the first to isolate and identify a dicaffeoylquinic acid compound from Artemisia selengensis leaves. The compound not only has an ability of inhibiting xanthine oxidase activity, but also has the function of anti-gout inflammation, which can be better used for treating gout. The dicaffeoylquinic acid is derived from natural plants and has the advantages of being safe and non-toxic, and having fewer side effects.

Abstract: The invention provides an AMH-INH-GNIH tri-expression gene vaccine capable of improving fecundity of animals and a preparation method of engineering strain thereof. The engineering strain was deposited in China Center for Type Culture Collection on Aug. 15, 2018, with deposit No.: CCTCC NO:M 2018544. When the engineering strain is used for direct immunization of animals or immunization of animals after being mixed with DNA vaccine adjuvant, the fecundity of the animals can be effectively improved. The tri-expression gene is a tri-expression non-resistant DNA plasmid of Mullerian duct resisting hormone, inhibin and gonadotropin release restraining hormone, which can be used for direct immunization of the animals through mucosa immunization to generate antibodies in the manner of being sprayed to noses, orally administered, blended into feeds and the like.

Abstract: A water resistant enhanced wound healing film includes components by mass volume concentration of: 5-10 mg/L of ultra-fine eggshell powder, 50-500 mg/L of ultramicroshell membrane powder or shell membrane soluble protein, 5-30 mg/L of lysozyme, 5-10 mg/L of chitosan, 0.05-5 mg/L of glycerin, and 0.1-5 mg/L of polymer polysaccharide. Also, a preparation method of the water resistant enhanced wound healing film is disclosed. The whole raw materials of the present invention are non-toxic and have good biocompatibility; the raw materials include eggshell, shell membrane and soluble mixed protein thereof, and lysozyme in residual egg white. In the present invention, various components of the eggshell waste are sufficiently used. The wound healing film has good water resistance, mechanical properties, antibacterial activity and protein absorption capacity, and low degradation rate.