Abstract: A disgregating device of biological material, comprising: a hollow outer body, defining an inner chamber; a fixed disgregating grid, having a plurality of microholes provided with sharp edges, and housed transversely in the inner chamber so as to define an upper loading chamber apt to be loaded with the biological material to be disgregated and a lower collecting chamber apt to collect the biological material, after it has been disgregated; and a bladed rotor, rotating in the inner chamber, apt to co-operate, rotating, with the fixed disgregating grid, so as to feed and bring the biological material, contained in the upper loading chamber, into contact with the microholes of the disgregating grid and therefore cause the disgregation of the biological material making it pass through these microholes.