Abstract: The invention discloses an exopolysaccharide from Rhodopseudomonas palustris and a method for preparing and use thereof, and the method for preparing comprises the steps of: 1) keeping a seed solution from Rhodopseudomonas palustris GJ-22 in a fermentation medium for fermentation culture to obtain a fermentation broth; 2) centrifuging the fermentation broth to take the supernatant, which is treated by alcohol precipitation after filtration, and then collecting the pellet from alcohol precipitation by centrifugation to obtain crude polysaccharide; 3) removing proteins from the rude polysaccharide using protease enzymolysis method and Sevag method, followed by dialysis treatment with distilled water to remove small molecules and organic solvent to obtain a polysaccharide sample; 4) purifying the polysaccharide sample through an anion exchange column and a molecular exclusion chromatography column obtain the exopolysaccharide from Rhodopseudomonas palustris.
Type:
Grant
Filed:
November 11, 2020
Date of Patent:
October 18, 2022
Assignees:
Hunan Plant Protection Institute, Changsha Agreen Bio-Tech Ltd., Co., Suzhou Ace Chemical Technology Co., Ltd.
Inventors:
Yong Liu, Pin Su, Zhongying Zhai, Zhuo Zhang, Haixing Yang, Jianping Dai, Bo Zhou, Deyong Zhang, Ju'e Cheng
Abstract: The invention discloses an exopolysaccharide from Rhodopseudomonas palustris and a method for preparing and use thereof, and the method for preparing comprises the steps of: 1) keeping a seed solution from Rhodopseudomonas palustris GJ-22 in a fermentation medium for fermentation culture to obtain a fermentation broth; 2) centrifuging the fermentation broth to take the supernatant, which is treated by alcohol precipitation after filtration, and then collecting the pellet from alcohol precipitation by centrifugation to obtain crude polysaccharide; 3) removing proteins from the rude polysaccharide using protease enzymolysis method and Sevag method, followed by dialysis treatment with distilled water to remove small molecules and organic solvent to obtain a polysaccharide sample; 4) purifying the polysaccharide sample through an anion exchange column and a molecular exclusion chromatography column obtain the exopolysaccharide from Rhodopseudomonas palustris.
Type:
Application
Filed:
November 20, 2020
Publication date:
February 10, 2022
Applicants:
Hunan Plant Protection Institute, Changsha Agreen Bio-Tech Ltd., Co., Suzhou Ace Chemical Technology Co., Ltd.
Inventors:
Yong LIU, Pin SU, Zhongying ZHAI, Zhuo ZHANG, Haixing YANG, Jianping DAI, Bo ZHOU, Deyong ZHANG, Ju'e CHENG