Abstract: A single molecule detection method based on grating scanning recognition, in which a large number of capture beads are scattered on a bead retaining plate (or a detection chip). At least part of the capture beads are specifically bound with an analyte, and each analyte is bound with a luminescent substance. Grating ports of a grating detection device are used to scan the capture beads column by column from the capture beads arranged at one end. The number of the capture beads bound with a marker is synchronously counted. The capture beads on the whole bead retaining plate are scanned through transverse relative movement between the grating ports and the capture beads. The total number of analytes can be obtained after scanning is completed.

Abstract: A method for mixing immunoassay reagent in immunoassay analysis device and an immunoassay analysis device are provided. The method disperses aggregated magnetic beads in a reagent tray of the immunoassay analysis device by keeping an immunoassay reagent tube containing the immunoassay reagent in a variable speed state during rotation and/or by exerting an impact force on the immunoassay reagent tube during rotation of the immunoassay reagent tube, so as to keep the immunoassay reagent in a mixed state. The method can mix the immunoassay reagent, avoid the phenomenon of magnetic beads' aggregation and ensure the accuracy of the testing results.

Abstract: Disclosed are a water-soluble fluorescent probe and nanoparticles with an aggregation induced emission (AIE) effect and preparation methods and the application. The fluorescent probe molecule includes one imidazole ring substituted by two methoxyphenyls and one phenyl and two quaternary ammonium salt structures. It has good dispersion in water, may form micelles. It has the characteristics of AIE effect and a large Stokes shift etc. It is suitable for fluorescence detection. Under the induction of lysophosphatidic acid, the probe can be self-assembled so as to aggregate and generate yellow fluorescence. Lysophosphatidic acid may be quantitatively analyzed by measuring the intensity of emitted fluorescence.