Patents Assigned to Hy-Gene, Inc.
  • Process and media for the growth of human cornea and gingiva
    Patent number: 5912175
    Abstract: Media and methods are disclosed for the in vitro formation of a histologically complete human epithelium. The media are serum-free, companion cell or feeder layer free and organotypic, matrix free solutions for the isolation and cultivation of clonally competent basal epithelial cells. The media and methods of the invention are useful in the production of epithelial tissues such as epidermis, cornea, gingiva and ureter.
    Type: Grant
    Filed: August 13, 1998
    Date of Patent: June 15, 1999
    Assignee: Hy-Gene, Inc.
    Inventor: John J. Wille, Jr.
  • Process and media for the growth of human epithelia
    Patent number: 5834312
    Abstract: Novel media and methods are disclosed for the in vitro formation of a histologically complete human epithelium. The media are serum-free, companion cell or feeder layer free and organotypic, matrix free solutions for the isolation and cultivation of clonally competent basal epithelial cells. The media and methods of the invention are useful in the production of epithelial tissues such as epidermis, cornea, gingiva and ureter.
    Type: Grant
    Filed: July 15, 1997
    Date of Patent: November 10, 1998
    Assignee: Hy-Gene, Inc.
    Inventor: John J. Wille, Jr.
  • Cell competency solution for use in the formation of a histologically-complete, living, human skin substitute
    Patent number: 5795781
    Abstract: Methods and formulations are disclosed for the in vitro formation of a histologically complete human epidermis in a serum-free, companion cell or cell feeder layer-free, and organotypic matrix-free culture system commencing with the isolation and cultivation of a unique population of clonally-competent basal epidermal cells and ending with the formation of a functional, histologically complete, human squamous epithelium. The formation of a histologically complete human epidermis is accomplished in a serum-free medium, without companion-cells or feeder layer cells or any organotypic support using a multi-step process that is controlled by manipulating the growth and differentiation factors requisite to the sequential development of a usable, functional, and completely differentiated epidermis.
    Type: Grant
    Filed: February 15, 1996
    Date of Patent: August 18, 1998
    Assignee: Hy-Gene, Inc.
    Inventor: John Jacob Wille, Jr.
  • Serum free medium for use in the formation of a histologically complete living human skin substitute
    Patent number: 5686307
    Abstract: Methods and formulations are disclosed for the in vitro formation of a histologically complete human epidermis in a serum-free, companion cell or cell feeder layer-free, and organotypic matrix-free culture system commencing with the isolation and cultivation of a unique population of clonally-competent basal epidermal cells and ending with the formation of a functional, histologically complete, human squamous epithelium. The formation of a histologically complete human epidermis is accomplished in a serum-free medium, without companion-cells or feeder layer cells or any organotypic support using a multi-step process that is controlled by manipulating the growth and differentiation factors requisite to the sequential development of a usable, functional, and completely differentiated epidermis.
    Type: Grant
    Filed: July 11, 1995
    Date of Patent: November 11, 1997
    Assignee: Hy-Gene, Inc.
    Inventor: John Jacob Wille, Jr.