Abstract: A recombinant chimeric polypeptide is produced that contains a biologically active entity and a binding entity, that displays biologically active and is inactivation resistant. The biologically active entity corresponds, for example, to an enzyme and the binding entity corresponds, for example, to an antibody. The entities interact to form a structure analagous to an antibody-antigen complex. Thus, the inactivation-resistant complex displays prolonged activity under conditions characterized by disrupting temperature, the presence of a proteolytic enzyme, disrupting pH, the presence of an oxidizing agent and/or the presence of an alcohol, among others.
Type:
Grant
Filed:
May 23, 1995
Date of Patent:
November 11, 1997
Assignee:
Hybrisens, Ltd.
Inventors:
Ezekiel Y. Shami, Aser Rothstein, Mohabir Ramjeesingh