Abstract: A process provides for measuring particles in suspension in a liquid in an particle response analyzing instrument comprising a cytometric flow cell head, wherein the suspension is injected into the head at an injection rate, the head comprises one or several sensors which deliver responses when measuring particles injected therein and which yield a significant response for a set measurement frequency range. The response is processed and used to generate results in the instrument. When a first response is significant, measurements continue at the same injection rate in order to obtain a result thats likely to continue being a significant response during subsequent measurements and, conversely, when the first response is not significant, the injection rate is modified in order to bring the response within a significant response range and to continue with measurements in order to obtain a result.
Abstract: Method for isolating and differentiating leucocytes in a blood sample, comprising the steps of:lysis of the erythrocytes with a solution whose osmolality and pH have been adjusted to maintain leucocyte integrity, and containing saponin,determination of the point of lysis, andinhibition of the lysis by diluting the sample with a solution having substantially a similar composition but not containing saponin.
Abstract: A method for differentiating and isolating sub-populations of leucocytes in a blood sample involved treating the sample with polyoxyethylene 9-lauryl ether. This method is suitable for isolating basophil granulocytes when it is conducted in an acid medium.