Abstract: A filter assembly for separating microcarriers from a fluid medium includes a collapsible container bounding a sterile compartment adapted to hold a fluid. An inlet port is attached to the container through which fluid flows into the compartment. An outlet port is attached to the container through which fluid flows out of the compartment. A filter is disposed within the compartment, the filter dividing the compartment into an inlet chamber that is fluidly coupled with the inlet port and an outlet chamber that is fluidly coupled with the outlet port, the filter allowing a medium to pass therethrough but preventing microcarriers disposed in the medium from passing therethrough.

Abstract: A system for forming a fluid connection includes a first connector and a second connector. Both connectors include a tubular body having a membrane mounted on a distal end thereof. A support member facilitates the coupling of the connectors together so that the membranes are abutted together. Radiant energy is applied to the abutted membranes so as to first sterilize the membranes and then melt the membranes so that a passage is formed therethrough.

Abstract: A fluid manifold system includes a manifold port assembly having a housing with an inlet port and a plurality of outlet ports formed thereon and a carousel having a plurality of spaced apart fluid paths that extend therethrough from a first end to an opposing second end. The carousel is rotatably coupled with the housing so that when the first end of each fluid path is aligned with the inlet port on the housing, the second end of each fluid path is aligned with a corresponding different one of the plurality of outlet ports. A container is fluid coupled to each outlet port by a fluid line. The inlet port can be selectively coupled with a fluid source while a motor can control rotation of the carousel relative to the housing.

Abstract: A quick coupling for a shaft includes a male coupling having a locking portion with a locking groove formed thereon. A female coupling includes a female body having an interior surface bounding a socket. A tapered port is formed on the female body and extending from the exterior surface to the interior surface thereof. The locking portion of the male coupling is received within the socket so that the tapered port is aligned with the locking groove. A ball bearing is disposed within the tapered port so that a portion of the ball bearing can project into the socket and into the locking groove. A locking sleeve is slidably mounted along the female body so as to cover and interact with the ball bearing.

Abstract: A reactor system includes a flexible bag having an interior surface bounding a compartment. An elongated tubular connector is disposed within the compartment of the flexible bag, the tubular connector having a first end and an opposing second end, the first end of the tubular connector being coupled with the flexible bag. An impeller is disposed within the compartment of the flexible bag and is secured to the tubular connector. A drive shaft is removably received within the tubular connector such that rotation of the drive shaft facilitates rotation of the impeller.

Abstract: A fluid heating system includes a tank assembly having an interior surface bounding a chamber, the tank assembly including a sidewall and a lid. A collapsible bag is removably disposed within the chamber of the tank assembly, the collapsible bag bounding a compartment adapted to hold a fluid. A boiler can be used for pumping heated fluid through the wall of the tank assembly for heating the fluid within the collapsible bag. An impeller is disposed within the compartment of the collapsible bag. An elongated drive shaft is removably coupled with the impeller such that rotation of the drive shaft facilitates rotation of the impeller within the compartment of the collapsible bag. A drive motor assembly is coupled with the drive shaft, the drive motor assembly being adapted to rotate the drive shaft when the lid is in an open or closed position.

Abstract: A sparger includes a body bounding a compartment. A tubular port or tube is coupled with the body. The tubular port or tub bounds a passage that communicates with the compartment of the body. At least a portion of the body includes of a first sparging sheet. The first sparging sheet includes a flexible sheet of a gas permeable material such that a gas passing into the compartment of the body through the passage can exit the compartment by permeating through the first sparging sheet.

Abstract: A method for processing a fluid includes sparging a fluid within the compartment of a container with an initial gas so that the initial gas passes through a portion of the fluid to form a humid gas within the compartment. The humid gas is passed out of the compartment of the container and into a flexible condenser bag. The humid gas within the condenser bag is cooled so as to separate the humid gas within the condenser bag into a condensed fluid and a dehumidified gas. The condensed fluid and the dehumidified gas is then removed from the condenser bag.

Abstract: A shaft coupling assembly includes a hub bounding an opening. The interior surface of the hub includes an annular coupling surface and a receiving surface, the receiving surface sloping away from opening. An alignment rib projects from the receiving surface into the opening. An elongated shaft includes a driver that is configured to be received within the opening of the hub. The driver has an exterior surface that includes an annular engaging surface, a guide surface, and a guide rib radially, outwardly projecting from the guide surface. The guide rib is configured such that when the driver is being inserted within the opening with the guide rib being aligned with the alignment rib, the guide rib contacts the alignment rib so that the guide rib must be offset from the alignment rib before the driver can be fully received within the opening.

Abstract: A system for mixing a liquid solution or suspension includes a support housing bounding a compartment and a collapsible bag disposed within the compartment. A mixer is disposed within the collapsible bag while a sparger delivers a gas to the lower end of the collapsible bag. A gas outlet line extends from an upper end of the collapsible bag to a condenser assembly. A gas exhaust line and a fluid collection line both extend from the condenser assembly.

Abstract: A port fitting for use with a barbed fluid connector includes a plug having a first end and an opposing second end with a guide outwardly projecting from the second end. An O-ring is disposed on and encircling the plug. A pair of elongated arms project alongside the length of the plug. Each arm has a first end secured to the first end of the plug and an opposing second end that is freely disposed so that the arms can be flexed by manipulation of the second end. Each arm has a catch that inwardly projects toward to the guide. In one embodiment the guide terminates at an end wall and a channel extends through the plug and guide to the end wall. An optical sensor is mounted on the end wall.

Abstract: A method for filling a dispensing bag includes inserting a collapsed bag within a chamber of a bin and securing a lower end of the collapsed bag to the bin. After securing the lower end of the collapsed bag to the bin, a hoist coupled to an upper end of the collapsed bag is manipulated so that the hoist raises the upper end of the collapsed bag within the chamber while the lower end of the collapsed bag remains secured to the bin so as to expand the collapsed bag within the chamber of the bin.

Abstract: A probe assembly includes a tubular sleeve having a passage extending between a first end and an opposing second end. The tubular sleeve is movable between an extended position wherein the first end and the opposing second end are spaced apart and a collapsed position wherein the first end and the opposing second end are moved closer together. A connector is secured to the second end of the tubular sleeve, the connector having an opening extending therethrough that communicates with the passage of the tubular sleeve, a sealing layer removably covering the opening of the connector. An elongated probe has a first end and an opposing second end, the second end of the probe being positioned within the passage of the tubular sleeve, the second end of the probe being configured to pass through the opening of the connector when the sealing layer is removed therefrom.

Abstract: A tube port includes a tubular first stem having an interior surface and an exterior surface extending between a first end and a longitudinally spaced apart second end. The interior surface bounds a passage that longitudinally extends through the first stem. The tube port also includes a flange encircling and radially outwardly projecting from the first end of the first stem. The flange and the first stem are integrally formed as a unitary member comprised of a flexible elastomeric material having a durometer on a Shore A scale with a value of less than 90.

Abstract: A method for filling a dispensing bag includes inserting a collapsed bag within the chamber of a bin, the chamber being bounded by a floor and an upstanding sidewall. A first port formed on the collapsed bag is securely fixed within a port hole formed on the floor of the bin. A hoist mounted on the bin is manipulated so that the hoist raises an upper end of the collapsed bag within the chamber while the first port remains fixed within the port hole so as to expand the collapsed bag within the chamber of the bin.

Abstract: A quick coupling for a shaft includes a male coupling having a locking portion with a locking groove formed thereon. A female coupling includes a female body having an interior surface bounding a socket. A tapered port is formed on the female body and extending from the exterior surface to the interior surface thereof. The locking portion of the male coupling is received within the socket so that the tapered port is aligned with the locking groove. A ball bearing is disposed within the tapered port so that a portion of the ball bearing can project into the socket and into the locking groove. A locking sleeve is slidably mounted along the female body so as to cover and interact with the ball bearing.

Abstract: A shaft coupling assembly includes a hub bounding an opening. The interior surface of the hub includes an annular coupling surface and a receiving surface, the receiving surface sloping away from opening. An alignment rib projects from the receiving surface into the opening. An elongated shaft includes a driver that is configured to be received within the opening of the hub. The driver has an exterior surface that includes an annular engaging surface, a guide surface, and a guide rib radially, outwardly projecting from the guide surface. The guide rib is configured such that when the driver is being inserted within the opening with the guide rib being aligned with the alignment rib, the guide rib contacts the alignment rib so that the guide rib must be offset from the alignment rib before the driver can be fully received within the opening.

Abstract: A system for forming a fluid connection includes a first connector and a second connector. Both connectors include a tubular body having a membrane mounted on a distal end thereof. A support member facilitates the coupling of the connectors together so that the membranes are abutted together. Radiant energy is applied to the abutted membranes so as to first sterilize the membranes and then melt the membranes so that a passage is formed therethrough.

Abstract: A probe assembly includes a tubular sleeve having a passage extending between a first end and an opposing second end. The tubular sleeve is movable between an extended position wherein the first end and the opposing second end are spaced apart and a collapsed position wherein the first end and the opposing second end are moved closer together. A connector is secured to the second end of the tubular sleeve, the connector having an opening extending therethrough that communicates with the passage of the tubular sleeve, a sealing layer removably covering the opening of the connector. An elongated probe has a first end and an opposing second end, the second end of the probe being positioned within the passage of the tubular sleeve, the second end of the probe being configured to pass through the opening of the connector when the sealing layer is removed therefrom.

Abstract: A tube port includes a tubular first stem having an interior surface and an exterior surface extending between a first end and a longitudinally spaced apart second end. The interior surface bounds a passage that longitudinally extends through the first stem. The tube port also includes a flange encircling and radially outwardly projecting from the first end of the first stem. The flange and the first stem are integrally formed as a unitary member comprised of a flexible elastomeric material having a durometer on a Shore A scale with a value of less than 90.