Abstract: A dual containment system is taught for adding sterile or caustic chemical, pharmaceutical, and biological fluids to and withdrawing them from a container. Included is a dual containment enabling fitting having a set of small diameter barbs at each end of a hollow conduit and a set of enlarged inner barbs at each end of a raised portion at the center of the hollow conduit. A port plate with a barbed spout is affixed to an aperture on the container. Also included is a dip tube with one end attached to a small diameter barb on the fitting and the other end extending into the container through the port plate spout. A connecting sheath encompasses the external portion of the dip tube and is attached at one end to a large diameter barb on the fitting and at the other end to the barbed spout. This creates a secondary containment chamber between the dip tube and the connecting sheath.

Abstract: The present invention provides media and methods for the maintenance or promotion of growth of a cellular line, particularly a hybridoma cellular line. A serum blend is disclosed comprising fetal bovine serum and agamma bovine calf serum. The serum blend is added to other types of support media and can be placed in association with a hybridoma cellular line. The cellular media are preferably employed with hybridoma cellular lines in an environment having a carbon dioxide atmosphere in the range from about ambient levels to 10%, and at a temperature in the range from about 32.degree. C. to 40.degree. C.In addition, the present invention provides for cellular lines maintained and grown in association with such a serum blend. Also, a serum used in a medium for producing diagnostic and therapeutic biologicals from cellular lines, particularly, hybridoma cellular lines, comprising a serum blend of fetal bovine serum and agamma bovine calf serum is provided.

Abstract: A cell culture media containment system is provided having a mixing bag substantially enclosed within a storage bag. Powdered cell culture media and other constituents are introduced into the mixing bag and are mixed therein. Thereafter, the reconstituted media is conveyed from the mixing bag into the storage bag undergoing sterilization during conveyance therebetween. The reconstituted cell culture media may then be stored in a sterilized condition in the storage bag or maybe dispensed into a cell growth container. In another embodiment of the present invention a plurality of storage bags or mixing bags may be utilized so that reconstituted cell culture media may be both mixed and dispensed while protein-laden cell culture media may be simultaneously harvested back into another of the plurality of storage bags.

Abstract: A multi-access port is provided having a mounting flange, an access cap, and a sidewall extending from the mounting flange to the access cap. Apertures are formed in the access cap in which are fitted tubing connectors having a lower tip extending below the access cap a distance less than the distance between the mounting flange and the access cap and having an upper tip extending above the access cap. A bottom surface of the mounting flange has formed therein channels capable of providing liquid communication therethrough when the bottom surface of the mounting flange is blocked or otherwise obscured by the bottom of the media bag in which the port is affixed. The multi-access port is typically mounted in the wall of a media bag such as media bags used in cell culture media containment system.