Patents Assigned to Hyglos Invest GmbH
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Publication number: 20210018495Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.Type: ApplicationFiled: September 29, 2020Publication date: January 21, 2021Applicant: HYGLOS INVEST GMBHInventors: Johannes REICH, Holger GRALLERT
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Patent number: 10697958Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.Type: GrantFiled: June 12, 2015Date of Patent: June 30, 2020Assignee: HYGLOS INVEST GMBHInventors: Johannes Reich, Holger Grallert
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Publication number: 20200166500Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.Type: ApplicationFiled: January 30, 2020Publication date: May 28, 2020Applicant: HYGLOS INVEST GMBHInventor: Bernd BUCHBERGER
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Patent number: 10585086Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.Type: GrantFiled: June 12, 2015Date of Patent: March 10, 2020Assignee: HYGLOS INVEST GMBHInventor: Bernd Buchberger
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Publication number: 20190353648Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.Type: ApplicationFiled: July 31, 2019Publication date: November 21, 2019Applicant: HYGLOS INVEST GMBHInventors: Johannes REICH, Holger GRALLERT
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Publication number: 20190256836Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.Type: ApplicationFiled: May 1, 2019Publication date: August 22, 2019Applicant: HYGLOS INVEST GMBHInventors: Bernd BUCHBERGER, Holger GRALLERT, Sonja MOLINARO
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Publication number: 20190187131Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.Type: ApplicationFiled: February 22, 2019Publication date: June 20, 2019Applicant: HYGLOS INVEST GMBHInventors: Johannes REICH, Holger GRALLERT
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Patent number: 10323238Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.Type: GrantFiled: June 28, 2017Date of Patent: June 18, 2019Assignee: HYGLOS INVEST GMBHInventors: Bernd Buchberger, Holger Grallert, Sonja Molinaro
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Publication number: 20170306315Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.Type: ApplicationFiled: June 28, 2017Publication date: October 26, 2017Applicant: HYGLOS INVEST GMBHInventors: Bernd BUCHBERGER, Holger GRALLERT, Sonja MOLINARO
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Publication number: 20170227529Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.Type: ApplicationFiled: June 12, 2015Publication date: August 10, 2017Applicant: HYGLOS INVEST GMBHInventor: Bernd BUCHBERGER
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Patent number: 9725706Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harboring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.Type: GrantFiled: December 4, 2013Date of Patent: August 8, 2017Assignee: HYGLOS INVEST GMBHInventors: Bernd Buchberger, Holger Grallert, Sonja Molinaro
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Publication number: 20170131265Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.Type: ApplicationFiled: June 12, 2015Publication date: May 11, 2017Applicant: HYGLOS INVEST GMBHInventors: Johannes REICH, Holger GRALLERT
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Publication number: 20160137993Abstract: The present invention relates to novel methods of generating and screening for chimeric polypeptides, which can be used in the treatment and prophylaxis of pathogenic bacterial contamination, colonisation and infection. The novel methods are based on random recombination of protein domains, and the chimeric polypeptides obtainable by the methods according to the invention are characterized in that they comprise at least one enzymatic active domain (EAD) and at least one cell binding domain (CBD). The present invention also relates to a library of chimeric polypeptides obtainable by the methods of the present invention.Type: ApplicationFiled: January 20, 2016Publication date: May 19, 2016Applicant: HYGLOS INVEST GMBHInventors: Anna Scherzinger, Sonja Molinaro, Bernd Buchberger
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Patent number: 9249447Abstract: The present invention relates to novel methods of generating and screening for chimeric polypeptides, which can be used in the treatment and prophylaxis of pathogenic bacterial contamination, colonization and infection. The novel methods are based on random recombination of protein domains, and the chimeric polypeptides obtainable by the methods according to the invention are characterized in that they comprise at least one enzymatic active domain (EAD) and at least one cell binding domain (CBD). The present invention also relates to a library of chimeric polypeptides obtainable by the methods of the present invention.Type: GrantFiled: June 17, 2011Date of Patent: February 2, 2016Assignee: HYGLOS INVEST GMBHInventors: Anna Scherzinger, Sonja Molinaro, Bernd Buchberger
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Patent number: 9229002Abstract: The present invention relates to bacteriophage tail proteins and the derivatives and fragments thereof that are capable of binding endotoxins in the absence of bivalent positive ions, especially Ca2+ or Mg2+. Further, the present invention relates to methods for depleting endotoxins from solutions and samples using the bacteriophage tail proteins according to the present invention and to a detection method for endotoxins.Type: GrantFiled: July 29, 2014Date of Patent: January 5, 2016Assignee: Hyglos Invest GmbHInventors: Stefan Miller, Roman Meyer, Renate Grassl, Manfred Biebl, Holger Grallert
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Patent number: 9057059Abstract: The present invention relates to chimeric polypeptides comprising a first portion, which comprises a bacteriocin cell wall-binding domain (CBD) and a second portion, which comprises an enzymatic active domain (EAD) selected from the lytic domain of a bacteriophage lysin, a bacteriocin and a bacterial autolysin. Provided are such chimeric polypeptides and variants and fragments thereof, nucleic acids encoding the same, vectors carrying such nucleic acids and host cells transformed or transfected with such vectors. The chimeric polypeptides of the present invention are useful for the reduction of certain bacterial populations, including methods and compositions for the treatment of various bacterial infections. For example, chimeric polypeptides of the present invention have been shown to effectively kill various bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), as well as other human pathogens.Type: GrantFiled: December 23, 2010Date of Patent: June 16, 2015Assignee: Hyglos Invest GmbHInventors: Holger Grallert, Sonja Molinaro
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Patent number: 9018155Abstract: The present invention relates to a polypeptide termed ply_pitti26 comprising the sequence as depicted in SEQ ID NO:1 as well as variants of this polypeptide. Furthermore, the present invention relates to nucleic acids and vectors encoding for said polypeptide and variants thereof as well as host cells comprising these nucleic acids and/or vectors. Finally, the present invention relates to the uses of said polypeptide, variants thereof, nucleic acid sequences, vectors and host cells, in particular for the treatment or prophylaxis of a subject infected by or exposed to Staphylococci.Type: GrantFiled: September 9, 2013Date of Patent: April 28, 2015Assignee: Hyglos Invest GmbHInventors: Michael Forchheim, Holger Grallert, Anja Philipp, Manfred Biebl
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Patent number: 8932580Abstract: The present invention relates to a method for the selective purification of bacterial cells and/or cell components, whereby the purification is performed by means of a solid support.Type: GrantFiled: July 28, 2009Date of Patent: January 13, 2015Assignees: Hyglos Invest GmbH, Biomerieux S.A.Inventors: Michael Schutz, Renate Grassl, Roman Meyer, Sibylle Frick, Ingrid Robl, Thomas Zander, Stefan Miller
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Publication number: 20140377775Abstract: The present invention relates to bacteriophage tail proteins and the derivatives and fragments thereof that are capable of binding endotoxins in the absence of bivalent positive ions, especially Ca2+ or Mg2+. Further, the present invention relates to methods for depleting endotoxins from solutions and samples using the bacteriophage tail proteins according to the present invention and to a detection method for endotoxins.Type: ApplicationFiled: July 29, 2014Publication date: December 25, 2014Applicant: HYGLOS INVEST GMBHInventors: Stefan MILLER, Roman MEYER, Renate GRASSL, Manfred BIEBL, Holger GRALLERT
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Patent number: 8822641Abstract: The present invention relates to bacteriophage tail proteins and the derivatives and fragments thereof that are capable of binding endotoxins in the absence of bivalent positive ions, especially Ca2+ or Mg2+. Further, the present invention relates to methods for depleting endotoxins from solutions and samples using the bacteriophage tail proteins according to the present invention and to a detection method for endotoxins.Type: GrantFiled: November 21, 2012Date of Patent: September 2, 2014Assignee: Hyglos Invest GmbHInventors: Stefan Miller, Roman Meyer, Renate Grassl, Manfred Biebl, Holger Grallert