Patents Assigned to Hyseq, Inc.
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Publication number: 20020058018Abstract: The present invention provides novel nucleic acids isolated from a cDNA library for fetal liver-spleen tissue, and the novel polypeptide sequences encoded by these nucleic acids. These novel polynucleotide and polypeptide sequences were determined to be a novel Interleukin-3.Type: ApplicationFiled: February 23, 2001Publication date: May 16, 2002Applicant: Hyseq, Inc.Inventor: John Ford
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Patent number: 6387645Abstract: The invention provides novel polynucleotides isolated from cDNA libraries of human fetal liver-spleen and macrophage as well as polypeptides encoded by these polynucleotides and mutants or variants thereof. The polypeptides correspond to a novel human CD39-like protein. Other aspects of the invention include vectors containing polynucleotides of the invention and related host cells as well a processes for producing novel CD39-like polypeptides, and antibodies specific for such polypeptides.Type: GrantFiled: July 9, 1999Date of Patent: May 14, 2002Assignee: Hyseq, Inc.Inventors: John Ford, Julio J. Mulero
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Publication number: 20020048760Abstract: The present invention provides methods and reagents useful for a wide range of nucleic acid sequence analyses, including sequencing by hybridization.Type: ApplicationFiled: April 2, 2001Publication date: April 25, 2002Applicant: Hyseq, Inc.Inventors: Snezana Drmanac, Chongjun Xu, Xiao-Hong Qian
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Patent number: 6372892Abstract: The present invention provides novel nucleic acids encoding IL-1 Hy2, a novel member of the Interleukin-1 Receptor Antagonist family, the novel polypeptides encoded by these nucleic acids and uses of these and related products.Type: GrantFiled: March 10, 2000Date of Patent: April 16, 2002Assignee: Hyseq, Inc.Inventors: Dennis G. Ballinger, Ann M. Pace, Hai Shan Lin
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Patent number: 6365726Abstract: The present invention provides novel nucleic acids encoding IL-1 Hy2, a novel member of the Interleukin-1 Receptor Antagonist family, the novel polypeptides encoded by these nucleic acids and uses of these and related products.Type: GrantFiled: May 22, 2000Date of Patent: April 2, 2002Assignee: Hyseq, Inc.Inventors: Dennis G. Ballinger, John Ford, Alice Suk-Yue Ho, Hai Shan Lin, Ann M. Pace
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Publication number: 20020034737Abstract: The invention relates to oligonucleotide probes attached to discrete particles wherein the particles can be grouped into a plurality of sets based on a physical property. A different probe is attached to the discrete particles of each set, and the identity of the probe is determined by identifying the discrete particles from their physical property. The physical property includes any that can be used to differentiate the discrete particles, and includes, for example, size, flourescence, radioactivity, electromagnetic charge, or absorbance, or label(s) may be attached to the particle such as a dye, a radionuclide, or an EML. In a preferred embodiment, discrete particles are separated by a flow cytometer which detects the size, charge, flourescence, or absorbance of the particle. The invention also relates to methods using the probes complexed with the discrete particles to analyze target nucleic acids.Type: ApplicationFiled: October 9, 1997Publication date: March 21, 2002Applicant: Hyseq, Inc.Inventor: RADOJE DRMANAC
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Patent number: 6355419Abstract: The invention relates to methods for preparing nucleic acid pools useful in hybridization studies. Such methods allow hybridization conditions, such as time, temperature, ionic strength, etc., to be adjusted to increase the likelihood that hybridization to the nucleic acids within each pool is within the linear range of detection (i.e., detectable but not saturating). The methods rely on pooling nucleic acids derived from a sample, based on the degree of representation within the sample, i.e., nucleic acids having similar degrees of representation within in a sample are combined into a pool. The invention also provides arrays and kits produced from pooled nucleic acids, and an improved method for identifying a nucleic acid and/or its representation in a sample.Type: GrantFiled: April 27, 1998Date of Patent: March 12, 2002Assignee: Hyseq, Inc.Inventor: Mark R. Alfenito
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Patent number: 6350447Abstract: The present invention provides novel polynucleotides and proteins encoded by such polynucleotides, along with therapeutic, diagnostic and research utilities for these polynucleotides and proteins. In particular, the polypeptides and polynucleotides of the invention comprise amino acid and nucleic acid sequences of novel CD39-like gene and gene products.Type: GrantFiled: January 29, 1999Date of Patent: February 26, 2002Assignee: HYSEQ, Inc.Inventors: Brian Paul Chadwick, Anna-Maria Frischauf
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Publication number: 20020012941Abstract: A method for identifying a unknown organism by comparison of the nucleotide sequence of nucleic acid in a sample with known nucleotide sequences and a determination that an unknown nucleotide sequence is present in the sample.Type: ApplicationFiled: September 18, 2001Publication date: January 31, 2002Applicant: Hyseq, Inc.Inventor: Lewis S. Gruber
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Patent number: 6337072Abstract: The present invention provides novel nucleic acids, the novel polypeptide sequences encoded by these nucleic acids and uses thereof. These novel polynucleotide and polypeptide sequences were determined to be a novel Interleukin-1 Receptor Antagonist.Type: GrantFiled: July 7, 1999Date of Patent: January 8, 2002Assignee: Hyseq, Inc.Inventors: John Ford, Ann Pace
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Patent number: 6335013Abstract: The invention provides novel polynucleotides isolated from cDNA libraries of human fetal liver-spleen and macrophage as well as polypeptides encoded by these polynucleotides and mutants or variants thereof. The polypeptides correspond to a novel human CD39-like protein. Other aspects of the invention include vectors containing polynucleotides of the invention and related host cells as well a processes for producing novel CD39-like polypeptides, and antibodies specific for such polypeptides.Type: GrantFiled: June 30, 2000Date of Patent: January 1, 2002Assignee: Hyseq, Inc.Inventors: John Ford, Julio J. Mulero, George Yeung
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Patent number: 6316191Abstract: The conditions under which oligonucleotide probes hybridize preferentially with entirely complementary and homologous nucleic acid targets are described. Using these hybridization conditions, overlapping oligonucleotide probes associate with a target nucleic acid. Following washes, positive hybridization signals are used to assemble the sequence of a given nucleic acid fragment. Representative target nucleic acids are applied as dots. Up to 100,000 probes of the type (A,T,C,G)(A,T,C,G)N8(A,T,C,G) are used to determine sequence information by simultaneous hybridization with nucleic acid molecules bound to a filter. Additional hybridization conditions are provided that allow stringent hybridization of 6-10 nucleotide long oligomers which extends the utility of the invention. A computer process determines the information sequence of the target nucleic acid which can include targets with the complexity of mammalian genomes.Type: GrantFiled: December 4, 1998Date of Patent: November 13, 2001Assignee: Hyseq, Inc.Inventors: Radoje T. Drmanac, Radomir B. Crkvenjakov
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Patent number: 6309824Abstract: The present invention provides a method for detecting a target nucleic acid species including the steps of providing an array of probes affixed to a substrate and a plurality of labeled probes wherein each labeled probe is selected to have a first nucleic acid sequence which is complementary to a first portion of a target nucleic acid and wherein the nucleic acid sequence of at least one probe affixed to the substrate is complementary to a second portion of the nucleic acid sequence of the target, the second portion being adjacent to the first portion; applying a target nucleic acid to the array under suitable conditions for hybridization of probe sequences to complementary sequences; introducing a labeled probe to the array; hybridizing a probe affixed to the substrate to the target nucleic acid; hybridizing the labeled probe to the target nucleic acid; affixing the labeled probe to an adjacently hybridized probe in the array; and detecting the labeled probe affixed to the probe in the array.Type: GrantFiled: January 16, 1997Date of Patent: October 30, 2001Assignee: Hyseq, Inc.Inventor: Radoje T. Drmanac
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Patent number: 6297006Abstract: The present invention provides a method for detecting a target nucleic acid species including the steps of providing an array of probes affixed to a substrate and a plurality of labeled probes wherein each labeled probe is selected to have a first nucleic acid sequence which is complementary to a first portion of a target nucleic acid and wherein the nucleic acid sequence of at least one probe affixed to the substrate is complementary to a second portion of the nucleic acid sequence of the target, the second portion being adjacent to the first portion; applying a target nucleic acid to the array under suitable conditions for hybridization of probe sequences to complementary sequences; introducing a labeled probe to the array; hybridizing a probe affixed to the substrate to the target nucleic acid; hybridizing the labeled probe to the target nucleic acid; affixing the labeled probe to an adjacently hybridized probe in the array; and detecting the labeled probe affixed to the probe in the array.Type: GrantFiled: March 4, 1997Date of Patent: October 2, 2001Assignee: Hyseq, Inc.Inventors: Radoje T. Drmanac, Snezana Drmanac, Aaron Hou, Brian Hauser
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Patent number: 6294655Abstract: The present invention provides novel nucleic acids, the novel polypeptide sequences encoded by these nucleic acids and uses thereof. These novel polynucleotide and polypeptide sequences were determined to be a novel Interleukin-1 Receptor Antagonist. Also provided are antibodies which bind the antagonist, methods of detecting the antagonist, and kits containing the antibodies.Type: GrantFiled: October 13, 1999Date of Patent: September 25, 2001Assignee: Hyseq, Inc.Inventors: John Ford, Ann Pace
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Patent number: 6270961Abstract: Sequencing by Hybridization (SBH) methods and apparatus employing subdivided filters for discrete multiple probe analysis of multiple samples may be used for DNA identification and for DNA sequencing. Partitioned filters are prepared. Samples are affixed to sections of partitioned filters and each sector is probed with a single probe or a multiplexed probe for hybridization scoring. Hybridization data is analyzed for probe complementarity, partial sequencing by SBH or complete sequencing by SBH.Type: GrantFiled: December 9, 1994Date of Patent: August 7, 2001Assignee: Hyseq, Inc.Inventor: Radoje Drmanac
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Patent number: 6268210Abstract: The present invention relates to spatially-addressable sandwich arrays of compounds, particularly biological compounds such as peptides and polynucleotide probes, and methods of making and using the same. The present invention also relates to a method and device for holding together the substrates of the sandwich array, more particularly, a clamping device for securely yet safely holding substrates of a sandwich array together during assembly, use, storage, and/or transport of the sandwich array.Type: GrantFiled: February 17, 1999Date of Patent: July 31, 2001Assignee: Hyseq, Inc.Inventors: Joerg Baier, Brian Hauser, Radoje T. Drmanac
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Patent number: 6255119Abstract: The present device is directed to a reagent transfer device for transferring a plurality of reagent samples from one location to another. A transfer plate has a top surface with reservoirs, a bottom surface with orifices, and channels connecting the reservoirs and orifices. Fluid is moved from the reservoirs, through the channels and out of the orifices to form samples on the bottom surface about the orifices. A movement apparatus is provided for bringing the samples into contact with deposit surfaces at various deposition positions. Arrays of at least 100 orifices per square centimeter are provided.Type: GrantFiled: September 25, 1998Date of Patent: July 3, 2001Assignee: Hyseq, Inc.Inventor: Joerg Baier
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Patent number: 6245518Abstract: The present invention relates to methods of using labeled tracers to generate spatially addressable arrays of immobilized molecules, particularly polynucleotides, that can be normalized for differences in immobilization efficiencies at different addresses in the array. It also relates to the arrays generated by the method and to use of these arrays to enhance discrimination in array-based assays, particularly the discrimination between perfectly matched hybrids and hybrids containing a single mismatch in nucleic acid hybridization assays.Type: GrantFiled: December 6, 1999Date of Patent: June 12, 2001Assignee: Hyseq, Inc.Inventor: Joerg Baier
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Patent number: 6107031Abstract: A method for identifying a unknown organism by comparison of the nucleotide sequence of nucleic acid in a sample with known nucleotide sequences and a determination that an unknown nucleotide sequence is present in the sample.Type: GrantFiled: February 18, 1997Date of Patent: August 22, 2000Assignee: Hyseq, Inc.Inventor: Lewis S. Gruber